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Abán, C.; Martinez, N.; Carou, C.; Albamonte, I.; Toro, A.; Seyahian, A.; Franchi, A.; Leguizamón, G.; Trigubo, D.; Damiano, A.; Farina, M. "Endocannabinoids participate in placental apoptosis induced by hypoxia inducible factor-1" (2016) Apoptosis. 21(10):1094-1105
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Abstract:

During pregnancy, apoptosis is a physiological event critical in the remodeling and aging of the placenta. Increasing evidence has pointed towards the relevance of endocannabinoids (ECs) and hypoxia as modulators of trophoblast cell death. However, the relation between these factors is still unknown. In this report, we evaluated the participation of ECs in placental apoptosis induced by cobalt chloride (CoCl2), a hypoxia mimicking agent that stabilizes the expression of hypoxia inducible factor-1 alpha (HIF-1α). We found that HIF-1α stabilization decreased FAAH mRNA and protein levels, suggesting an increase in ECs tone. Additionally, CoCl2 incubation and Met-AEA treatment reduced cell viability and increased TUNEL-positive staining in syncytiotrophoblast layer. Immunohistochemical analysis demonstrated Bax and Bcl-2 protein expression in the cytoplasm of syncytiotrophoblast. Finally, HIF-1α stabilization produced an increase in Bax/Bcl-2 ratio, activation of caspase 3 and PARP cleavage. All these changes in apoptotic parameters were reversed with AM251, a CB1 antagonist. These results demonstrate that HIF-1α may induce apoptosis in human placenta via intrinsic pathway by a mechanism that involves activation of CB1 receptor suggesting a role of the ECs in this process. © 2016, Springer Science+Business Media New York.

Registro:

Documento: Artículo
Título:Endocannabinoids participate in placental apoptosis induced by hypoxia inducible factor-1
Autor:Abán, C.; Martinez, N.; Carou, C.; Albamonte, I.; Toro, A.; Seyahian, A.; Franchi, A.; Leguizamón, G.; Trigubo, D.; Damiano, A.; Farina, M.
Filiación:Laboratorio de Fisiopatología Placentaria, Centro de Estudios Farmacológicos y Botánicos (CEFYBO, CONICET), Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, 16th floor, Buenos Aires, C1121ABG, Argentina
Laboratorio de Biología de la Reproducción, Instituto de Fisiología y Biofísica Bernardo Houssay (IFIBIO) - CONICET- Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
Cátedra de Histología y Embriología, Facultad de Ciencias Veterinarias, UBA, Buenos Aires, Argentina
Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y Diagnóstico – CEBBAD, Universidad Maimónides, Buenos Aires, Argentina
Departamento de Química Biológica, Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales, Facultad de Ciencias Exactas y Naturales, UBA, Buenos Aires, Argentina
Laboratorio de Fisiopatología de la Preñez y el Parto, (CEFYBO, CONICET), Facultad de Medicina, UBA, Buenos Aires, Argentina
Departamento de Obstetricia y Ginecología, Unidad de Embarazo de Alto Riesgo, Centro de Educación Médica e Investigaciones Clínicas (CEMIC), Buenos Aires, Argentina
Departamento de Ciencias Biológicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Apoptosis; Endocannabinoids; Hipoxia inducible factor 1; Placenta; cannabinoid 1 receptor; caspase 3; chorionic gonadotropin beta subunit; endocannabinoid; fatty acid amidase; hypoxia inducible factor 1; lactate dehydrogenase; messenger RNA; nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase; protein Bax; protein bcl 2; BCL2 protein, human; CASP3 protein, human; caspase 3; cobalt; cobalt chloride; endocannabinoid; hypoxia inducible factor 1alpha; protein bcl 2; apoptosis; Article; cell viability; controlled study; enzyme activation; female; hormone synthesis; human; human tissue; hypoxia; immunoreactivity; intracellular signaling; placenta; placenta tissue; priority journal; protein cleavage; protein expression; protein induction; protein localization; protein stability; syncytiotrophoblast; upregulation; cell hypoxia; cell survival; cytology; drug effects; genetics; metabolism; placenta; pregnancy; signal transduction; Apoptosis; Caspase 3; Cell Hypoxia; Cell Survival; Cobalt; Endocannabinoids; Female; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; Placenta; Pregnancy; Proto-Oncogene Proteins c-bcl-2; Signal Transduction
Año:2016
Volumen:21
Número:10
Página de inicio:1094
Página de fin:1105
DOI: http://dx.doi.org/10.1007/s10495-016-1274-x
Título revista:Apoptosis
Título revista abreviado:Apoptosis
ISSN:13608185
CODEN:APOPF
CAS:caspase 3, 169592-56-7; fatty acid amidase, 153301-19-0; lactate dehydrogenase, 9001-60-9; nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase, 58319-92-9; protein bcl 2, 219306-68-0; cobalt, 7440-48-4; cobalt chloride, 1332-82-7, 7646-79-9; BCL2 protein, human; CASP3 protein, human; Caspase 3; Cobalt; cobaltous chloride; Endocannabinoids; Hypoxia-Inducible Factor 1, alpha Subunit; Proto-Oncogene Proteins c-bcl-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13608185_v21_n10_p1094_Aban

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Citas:

---------- APA ----------
Abán, C., Martinez, N., Carou, C., Albamonte, I., Toro, A., Seyahian, A., Franchi, A.,..., Farina, M. (2016) . Endocannabinoids participate in placental apoptosis induced by hypoxia inducible factor-1. Apoptosis, 21(10), 1094-1105.
http://dx.doi.org/10.1007/s10495-016-1274-x
---------- CHICAGO ----------
Abán, C., Martinez, N., Carou, C., Albamonte, I., Toro, A., Seyahian, A., et al. "Endocannabinoids participate in placental apoptosis induced by hypoxia inducible factor-1" . Apoptosis 21, no. 10 (2016) : 1094-1105.
http://dx.doi.org/10.1007/s10495-016-1274-x
---------- MLA ----------
Abán, C., Martinez, N., Carou, C., Albamonte, I., Toro, A., Seyahian, A., et al. "Endocannabinoids participate in placental apoptosis induced by hypoxia inducible factor-1" . Apoptosis, vol. 21, no. 10, 2016, pp. 1094-1105.
http://dx.doi.org/10.1007/s10495-016-1274-x
---------- VANCOUVER ----------
Abán, C., Martinez, N., Carou, C., Albamonte, I., Toro, A., Seyahian, A., et al. Endocannabinoids participate in placental apoptosis induced by hypoxia inducible factor-1. Apoptosis. 2016;21(10):1094-1105.
http://dx.doi.org/10.1007/s10495-016-1274-x