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Abstract:

Laccases are multicopper polyphenol oxidases that are able to catalyze the oxidation of a wide range of phenolic compounds with the simultaneous reduction of O2 to H2O. Despite their promising industrial uses, feasible incorporation of laccases in harsh processes requires the bioprospecting and/or engineering of enzymes to be stable and active in acidic or alkaline pHs, high temperatures, oxidative conditions and tolerant to high salinity and/or organic solvents. Here we used a PCR-based screening to clone two novel laccase coding sequences from the white-rot basidiomycete Trametes trogii. Recombinant expression of lcc3 gene in Komagataella (=Pichia) pastoris showed that it encodes a thermo active and thermostable laccase with an optimum temperature of 50 °C and with a half-life of 45 min at 70 °C and a stability higher than 3 h at 60 °C. Furthermore, recombinant LCC3 was capable of decolorizing between 50% and 100% of indigoid, triarylmethane, azoic and anthraquinonic synthetic dyes in the presence of the natural redox mediator acetosyringone within 2 h of incubation at pH 6 and 70 °C. © 2016 Elsevier Ltd.

Registro:

Documento: Artículo
Título:Heterologous production, characterization and dye decolorization ability of a novel thermostable laccase isoenzyme from Trametes trogii BAFC 463
Autor:Campos, P.A.; Levin, L.N.; Wirth, S.A.
Filiación:Laboratorio de Agrobiotecnología, Departamento de Fisiología Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IBBEA-CONICET-UBA, Pabellón 2 Ciudad Universitaria, Piso 2, Buenos Aires, C1428EGA, Argentina
Laboratorio de Micología Experimental, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, PROPLAME-PRHIDEB-CONICET, Pabellón 2 Ciudad Universitaria, Piso 4, Buenos Aires, C1428EGA, Argentina
Palabras clave:Acetosyringone; Dye decolorization; Pichia pastoris; Thermostable laccase; Trametes trogii; Alkalinity; C (programming language); Gene expression; Acetosyringone; Dye decolorization; Laccases; Pichia Pastoris; Trametes trogii; Enzymes
Año:2016
Volumen:51
Número:7
Página de inicio:895
Página de fin:903
DOI: http://dx.doi.org/10.1016/j.procbio.2016.03.015
Título revista:Process Biochemistry
Título revista abreviado:Process Biochem.
ISSN:13595113
CODEN:PBCHE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13595113_v51_n7_p895_Campos

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Citas:

---------- APA ----------
Campos, P.A., Levin, L.N. & Wirth, S.A. (2016) . Heterologous production, characterization and dye decolorization ability of a novel thermostable laccase isoenzyme from Trametes trogii BAFC 463. Process Biochemistry, 51(7), 895-903.
http://dx.doi.org/10.1016/j.procbio.2016.03.015
---------- CHICAGO ----------
Campos, P.A., Levin, L.N., Wirth, S.A. "Heterologous production, characterization and dye decolorization ability of a novel thermostable laccase isoenzyme from Trametes trogii BAFC 463" . Process Biochemistry 51, no. 7 (2016) : 895-903.
http://dx.doi.org/10.1016/j.procbio.2016.03.015
---------- MLA ----------
Campos, P.A., Levin, L.N., Wirth, S.A. "Heterologous production, characterization and dye decolorization ability of a novel thermostable laccase isoenzyme from Trametes trogii BAFC 463" . Process Biochemistry, vol. 51, no. 7, 2016, pp. 895-903.
http://dx.doi.org/10.1016/j.procbio.2016.03.015
---------- VANCOUVER ----------
Campos, P.A., Levin, L.N., Wirth, S.A. Heterologous production, characterization and dye decolorization ability of a novel thermostable laccase isoenzyme from Trametes trogii BAFC 463. Process Biochem. 2016;51(7):895-903.
http://dx.doi.org/10.1016/j.procbio.2016.03.015