Artículo

La versión final de este artículo es de uso interno de la institución.
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

To investigate the growth and production of lignocellulosic enzymes from Lentinus tigrinus, solid state fermentation (SSF) during 110 days was performed by using wheat straw as substrate. The BE obtained was 62% and three flushes of mushrooms were harvested. L. tigrinus showed the capacity to degrade wheat straw, causing a 21.49% decrease in lignin content and a 53.26% decrease in cellulose. The water extractives (aromatic compounds and reducing sugars) increased to 121.44% along incubation time. All of the enzyme activities were high during colonization and ligninases declined drastically during fruit body formation. After harvesting the last flush, enzyme activities increased rapidly to highest levels, except for laccase activity. Cellulases showed the maximum of activity around 90 days post inoculation. Laccase titres reached a peak of 30 U g-1 at day 20 and MnP had two peaks of around 750 mU g-1 at days 20 and 90. Aromatic compounds from lignin degradation were not inducers of ligninases. Laccase was the most stable enzyme showing complete stability after 42 h at 40°C. All enzymes showed acid optimum pH. The minor value was recorded for laccase and endoglucanase (pH 3.0). © 2005 Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:Production of lignocellulosic enzymes during growth and fruiting of the edible fungus Lentinus tigrinus on wheat straw
Autor:Lechner, B.E.; Papinutti, V.L.
Filiación:Laboratorio de Micología Experimental, Departamento de Biodiversidad Y Biología Experimental, Ciudad Universitaria Pab. II, (1428) Buenos Aires, Argentina
Palabras clave:Cellulases; Ligninases; SSF; White-rot fungi; Xylanases; Fermentation; Fungi; pH effects; Substrates; Solid state fermentation; SSF; White rot fungi; Xylanases; Enzymes; Basidiomycota; Fungi; Lentinus; Lentinus tigrinus; Phanerochaete; Triticum aestivum
Año:2006
Volumen:41
Número:3
Página de inicio:594
Página de fin:598
DOI: http://dx.doi.org/10.1016/j.procbio.2005.08.004
Título revista:Process Biochemistry
Título revista abreviado:Process Biochem.
ISSN:13595113
CODEN:PBCHE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13595113_v41_n3_p594_Lechner

Referencias:

  • Chang, S.T., World production of cultivated edible and medicinal mushrooms in 1997 with emphasis on Lentinus edodes (Berk.) Sing. in China (1999) Int J Med Mushrooms, 1, pp. 291-300
  • Stamets, P.S., (1993) Growing Gourmet and Medicinal Mushrooms, , Ten Speed Press Berkeley
  • Sobal, M., Morales, P., Martínez, W., Martínez, D.N., Martínez-Carrera, D., Cultivation of Lentinus levis in México (1997) Micol Neotrop Apl, 10, pp. 63-71
  • Khurana, H.L., Kaul, T.N., Kachroo, J.L., Nutritional value of wild edible mushrooms from Kashmir-1 (1983) Indian Mushroom Science II, pp. 184-189. , Kaul TN, Kapur BM, editors
  • Mester, T., Ambert-Balay, K., Ciofi-Baffoni, S., Banci, L., Jones, A.D., Tien, M., Oxidation of a tetrameric nonphenolic lignin model compound by lignin peroxidase (2001) J Biol Chem, 276, pp. 22985-22990
  • Magnelli, P., Forchiassin, F., Regulation of the cellulase complex production by Saccobolus saccoboloides: Induction and repression by carbohydrates (1999) Mycologia, 91, pp. 359-364
  • Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal Biochem, 72, pp. 248-254
  • Nelson, N.J., A photometric adaptation of the Somogyi method for the determination of glucose (1944) J Biochem, 153, pp. 375-380
  • (1983) Tappi Methods. Acid Insoluble Lignin in Wood and Pulp, , T222 om-83;
  • Muñoz, C., Guillén, F., Martínez, A.T., Martínez, M.J., Induction and characterization of laccase in the ligninolytic fungus Pleurotus eryngii (1997) Curr Microbiol, 34, pp. 1-5
  • Glenn, J.K., Gold, M.H., Purification and characterisation of an extracellular Mn(II)-dependent peroxidase from the lignin degrading basidiomycete Phanerochaete chrysosporium (1985) Arch Biochem Biophys, 242, pp. 329-341
  • Metha, V., Gupta, J.K., Kaushal, S.C., Cultivation of Pleurotus florida mushroom on rice straw and biogas production from the spend straw (1990) World J Microb Biotech, 6, pp. 366-370
  • Chang, S.T., Miles, P.G., Recent trends in world production of cultivated mushrooms (1991) Mushroom J, 503, pp. 15-18
  • Ragunathan, R., Swaminathan, K., Nutritional status of Pleurotus spp. grown on various agro-wastes (2003) Food Chem, 80, pp. 371-375
  • Kues, U., Liu, Y., Fruit body production in basidiomycetes (2000) Appl Microbiol Biotechnol, 54, pp. 141-152
  • Kalmis, E., Sargin, S., Cultivation of two Pleurotus species on wheat straw substrates containing olive mill waste water (2004) Int Biodet Biodegr, 53, pp. 43-47
  • Zhang, R., Li, X., Fadel, J.G., Oyster mushroom cultivation with rice and wheat straw (2002) Biores Technol, 82, pp. 277-284
  • Nazareth, S.W., Sampy, J.D., Production and characterisation of lignocellulases of Panus tigrinus and their application (2003) Int Biodet Biodegr, 52, pp. 207-214
  • Wainright, M., Novel industrial uses for fungi (1992) Introduction to Fungal Biotechnology, pp. 67-78. , Wiley Interscience UK
  • Pointing, S.B., Jones, E.B.G., Vrijmoed, L.L.P., Optimization of laccase production by Pycnoporus sanguineus in submerged liquid cultures (2000) Mycologia, 92, pp. 139-144
  • Muñoz, C., Guillén, F., Martínez, A.T., Martínez, M.J., Laccase isoenzymes of Pleurotus eryngii: Characterization, catalytic properties, and participation in activation of molecular oxygen and Mn 2+ oxidation (1997) Appl Environ Microbiol, 63, pp. 2166-2174
  • Rogalski, J., Lundell, T., Leonowicz, A., Hatakka, A.I., Influence of aromatic compounds and lignin on production of ligninolytic enzymes by Phlebia radiata (1991) Phytochemistry, 30, pp. 2869-2872
  • Kapich, A.N., Prior, B.A., Botha, A., Galkin, S., Hatakka, A., Effect of lignocellulose-containing substrates on production of ligninolytic peroxidases in submerged cultures of Phanerochaete chrysosporium ME-446 (2004) Enzyme Microb Technol, 34, pp. 187-195
  • D'Souza, T.M., Merritt, C.S., Reddy, C.A., Lignin-modifying enzymes of the white rot basidiomycete Ganoderma lucidum (1999) Appl Environ Microbiol, 65, pp. 5307-5313
  • Eggert, C., Temp, U., Eriksson, K.E., The ligninolytic system of the white rot fungus Pycnoporus cinnabarinus: Purification and characterization of the laccase (1996) Appl Environ Microbiol, 62, pp. 1151-1158
  • Kirk, T.K., Connors, W.J., Zeikus, J.G., Requirement for a growth substrate during lignin decomposition by two wood-roting fungi (1976) Appl Environ Microbiol, 32, pp. 192-194
  • Leatham, G.F., Extracellular enzymes produced by the cultivated mushroom Lentinus edodes during degradation of lignocellulosic medium (1985) Appl Environ Microbiol, 50, pp. 859-867
  • Leatham, G.F., Stahmann, M.A., Effect of light and aeration on fruiting of Lentinula edodes (1987) Trans Br Mycol Soc, 88, pp. 9-20
  • Papinutti, V.L., Diorio, L.A., Forchiassin, F., Production of laccase and manganese peroxidase by Fomes sclerodermeus grown on wheat bran (2003) J Ind Microbiol Biotechnol, 30, pp. 157-160
  • Ohga, S., Royse, D.J., Transcriptional regulation of laccase and cellulase genes during growth and fruiting of Lentinula edodes on supplemented sawdust (2001) FEMS Microbiol Lett, 201, pp. 111-115
  • Wood, D.A., Goodenough, P.W., Fruiting of A. bisporus: Changes in extracellular enzyme activities during growth and fruiting (1977) Arch Microbiol, 114, pp. 161-165
  • Manning, K., Wood, D.A., Production and regulation of cellulase of A. bisporus (1983) J Gen Microbiol, 129, pp. 1839-1847
  • Ohga, S., Comparison of extracellular enzyme activities among different strains of L. edodes grown on sawdust-based cultures in relationship to the fruiting abilities (1992) J Japan Wood Res Soc, 38, pp. 310-316

Citas:

---------- APA ----------
Lechner, B.E. & Papinutti, V.L. (2006) . Production of lignocellulosic enzymes during growth and fruiting of the edible fungus Lentinus tigrinus on wheat straw. Process Biochemistry, 41(3), 594-598.
http://dx.doi.org/10.1016/j.procbio.2005.08.004
---------- CHICAGO ----------
Lechner, B.E., Papinutti, V.L. "Production of lignocellulosic enzymes during growth and fruiting of the edible fungus Lentinus tigrinus on wheat straw" . Process Biochemistry 41, no. 3 (2006) : 594-598.
http://dx.doi.org/10.1016/j.procbio.2005.08.004
---------- MLA ----------
Lechner, B.E., Papinutti, V.L. "Production of lignocellulosic enzymes during growth and fruiting of the edible fungus Lentinus tigrinus on wheat straw" . Process Biochemistry, vol. 41, no. 3, 2006, pp. 594-598.
http://dx.doi.org/10.1016/j.procbio.2005.08.004
---------- VANCOUVER ----------
Lechner, B.E., Papinutti, V.L. Production of lignocellulosic enzymes during growth and fruiting of the edible fungus Lentinus tigrinus on wheat straw. Process Biochem. 2006;41(3):594-598.
http://dx.doi.org/10.1016/j.procbio.2005.08.004