Artículo

Ceruti, J.M.; Scassa, M.E.; Marazita, M.C.; Carcagno, A.C.; Sirkin, P.F.; Cánepa, E.T. "Transcriptional upregulation of p19INK4d upon diverse genotoxic stress is critical for optimal DNA damage response" (2009) International Journal of Biochemistry and Cell Biology. 41(6):1344-1353
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Abstract:

p19INK4d promotes survival of several cell lines after UV irradiation due to enhanced DNA repair, independently of CDK4 inhibition. To further understand the action of p19INK4d in the cellular response to DNA damage, we aimed to elucidate whether this novel regulator plays a role only in mechanisms triggered by UV or participates in diverse mechanisms initiated by different genotoxics. We found that p19INK4d is induced in cells injured with cisplatin or β-amyloid peptide as robustly as with UV. The mentioned genotoxics transcriptionally activate p19INK4d expression as demonstrated by run-on assay without influencing its mRNA stability and with partial requirement of protein synthesis. It is not currently known whether DNA damage-inducible genes are turned on by the DNA damage itself or by the consequences of that damage. Experiments carried out in cells transfected with distinct damaged DNA structures revealed that the damage itself is not responsible for the observed up-regulation. It is also not known whether the increased expression of DNA-damage-inducible genes is related to immediate protective responses such as DNA repair or to more delayed responses such as cell cycle arrest or apoptosis. We found that ectopic expression of p19INK4d improves DNA repair ability and protects neuroblastoma cells from apoptosis caused by cisplatin or β-amyloid peptide. Using clonal cell lines where p19INK4d levels can be modified at will, we show that p19INK4d expression correlates with increased survival and clonogenicity. The results presented here, prompted us to suggest that p19INK4d displays an important role in an early stage of cellular DNA damage response. © 2008 Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:Transcriptional upregulation of p19INK4d upon diverse genotoxic stress is critical for optimal DNA damage response
Autor:Ceruti, J.M.; Scassa, M.E.; Marazita, M.C.; Carcagno, A.C.; Sirkin, P.F.; Cánepa, E.T.
Filiación:Laboratorio de Biología Molecular, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria Pabellon II Piso 4, 1428 Buenos Aires, Argentina
Palabras clave:Apoptosis; Cell survival; DNA damage; Genotoxic stress; INK4; amyloid beta protein; cisplatin; cyclin dependent kinase inhibitor 2D; messenger RNA; animal cell; apoptosis; article; cell cycle arrest; cell damage; cell survival; clonogenesis; controlled study; DNA damage; DNA repair; DNA structure; enzyme induction; gene expression; genetic transfection; genotoxicity; molecular mechanics; neuroblastoma cell; nonhuman; protein expression; protein synthesis; RNA stability; transcription initiation; ultraviolet radiation; upregulation; Amyloid beta-Protein; Animals; Apoptosis; Blotting, Northern; Cell Line; Cell Survival; Cisplatin; Cricetinae; Cyclin-Dependent Kinase Inhibitor p19; DNA Damage; DNA Repair; Humans; Neuroblastoma; RNA, Messenger; Transcriptional Activation; Transfection; Tumor Suppressor Protein p53; Ultraviolet Rays; Up-Regulation
Año:2009
Volumen:41
Número:6
Página de inicio:1344
Página de fin:1353
DOI: http://dx.doi.org/10.1016/j.biocel.2008.12.005
Título revista:International Journal of Biochemistry and Cell Biology
Título revista abreviado:Int. J. Biochem. Cell Biol.
ISSN:13572725
CODEN:IJBBF
CAS:amyloid beta protein, 109770-29-8; cisplatin, 15663-27-1, 26035-31-4, 96081-74-2; Amyloid beta-Protein; Cisplatin, 15663-27-1; Cyclin-Dependent Kinase Inhibitor p19; RNA, Messenger; Tumor Suppressor Protein p53
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13572725_v41_n6_p1344_Ceruti

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Citas:

---------- APA ----------
Ceruti, J.M., Scassa, M.E., Marazita, M.C., Carcagno, A.C., Sirkin, P.F. & Cánepa, E.T. (2009) . Transcriptional upregulation of p19INK4d upon diverse genotoxic stress is critical for optimal DNA damage response. International Journal of Biochemistry and Cell Biology, 41(6), 1344-1353.
http://dx.doi.org/10.1016/j.biocel.2008.12.005
---------- CHICAGO ----------
Ceruti, J.M., Scassa, M.E., Marazita, M.C., Carcagno, A.C., Sirkin, P.F., Cánepa, E.T. "Transcriptional upregulation of p19INK4d upon diverse genotoxic stress is critical for optimal DNA damage response" . International Journal of Biochemistry and Cell Biology 41, no. 6 (2009) : 1344-1353.
http://dx.doi.org/10.1016/j.biocel.2008.12.005
---------- MLA ----------
Ceruti, J.M., Scassa, M.E., Marazita, M.C., Carcagno, A.C., Sirkin, P.F., Cánepa, E.T. "Transcriptional upregulation of p19INK4d upon diverse genotoxic stress is critical for optimal DNA damage response" . International Journal of Biochemistry and Cell Biology, vol. 41, no. 6, 2009, pp. 1344-1353.
http://dx.doi.org/10.1016/j.biocel.2008.12.005
---------- VANCOUVER ----------
Ceruti, J.M., Scassa, M.E., Marazita, M.C., Carcagno, A.C., Sirkin, P.F., Cánepa, E.T. Transcriptional upregulation of p19INK4d upon diverse genotoxic stress is critical for optimal DNA damage response. Int. J. Biochem. Cell Biol. 2009;41(6):1344-1353.
http://dx.doi.org/10.1016/j.biocel.2008.12.005