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Lombardo, M.E.; Araujo, L.S.; Batlle, A. "5-Aminolevulinic acid synthesis in epimastigotes of Trypanosoma cruzi" (2003) International Journal of Biochemistry and Cell Biology. 35(8):1263-1271
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Abstract:

Background and aims: Trypanosoma cruzi is the causative agent of Chagas disease or American trypanosomiasis. The parasite manifests a nutritional requirement for heme compounds because of its biosynthesis deficiency. The aim of this study has been to investigate the presence of metabolites and enzymes of porphyrin pathway, as well as ALA formation in epimastigotes of T. cruzi, Tulahuén strain, Tul 2 stock. Methods: Succinyl CoA synthetase, 5-aminolevulinic acid (ALA) synthetase, 4,5-dioxovaleric (DOVA) transaminase, ALA dehydratase and porphobilinogenase activities, as well as ALA, porphobilinogen (PBG), free porphyrins and heme content were measured in a parasite cells-free extract. Extracellular content of these metabolites was also determined. Results: DOVA, PBG, porphyrins and heme were not detected in acellular extracts of T. cruzi. However ALA was detected both intra- and extracellularly This is the first time that the presence of ALA (98% of intracellularly formed ALA) is demonstrated in the extracellular medium of a parasite culture. Regarding the ALA synthesizing enzymes, DOVA transaminase levels found were low (7.13±0.49EU/mg protein), whilst ALA synthetase (ALA-S) activity was undetectable. A compound of non-protein nature, low molecular weight, heat unstable, inhibiting bacterial ALA-S activity was detected in an acellular extract of T. cruzi. This inhibitor could not be identified with either ALA, DOVA or heme. Conclusions: ALA synthesis is functional in the parasite and it would be regulated by the heme levels, both directly and through the inhibitor factor detected. ALA formed can not be metabolized further, because the necessary enzymes are not active, therefore it should be excreted to avoid intracellular cytotoxicity. © 2003 Elsevier Science Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:5-Aminolevulinic acid synthesis in epimastigotes of Trypanosoma cruzi
Autor:Lombardo, M.E.; Araujo, L.S.; Batlle, A.
Filiación:Ctro. Invest. Porfirinas y Porfirias, Cd. Univ. Pabellón II 2do, Piso, 1428 Buenos Aires, Argentina
Viamonte 1881, 10 A CP-1056 Buenos Aires, Argentina
Palabras clave:5-Aminolevulinic acid; 5-Aminolevulinic acid synthetase inhibition; Heme synthesis; Trypanosoma cruzi; 4,5 dioxovaleric transaminase; 5 aminolevulinate synthase; aminolevulinic acid; aminotransferase; bacterial enzyme; heme; porphobilinogen; porphobilinogen synthase; porphyrin; porphyrin derivative; succinyl coenzyme A synthetase; unclassified drug; amino acid metabolism; article; cell free system; cytotoxicity; enzyme activity; epimastigote; metabolite; molecular weight; nonhuman; synthesis; thermostability; Trypanosoma cruzi; Bacteria (microorganisms); Trypanosoma; Trypanosoma cruzi; Trypanosoma cruzi
Año:2003
Volumen:35
Número:8
Página de inicio:1263
Página de fin:1271
DOI: http://dx.doi.org/10.1016/S1357-2725(03)00033-5
Título revista:International Journal of Biochemistry and Cell Biology
Título revista abreviado:Int. J. Biochem. Cell Biol.
ISSN:13572725
CODEN:IJBBF
CAS:5 aminolevulinate synthase, 9037-14-3; aminolevulinic acid, 106-60-5; aminotransferase, 9031-66-7; heme, 14875-96-8; porphobilinogen, 487-90-1; porphobilinogen synthase, 9036-37-7; porphyrin, 24869-67-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13572725_v35_n8_p1263_Lombardo

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Citas:

---------- APA ----------
Lombardo, M.E., Araujo, L.S. & Batlle, A. (2003) . 5-Aminolevulinic acid synthesis in epimastigotes of Trypanosoma cruzi. International Journal of Biochemistry and Cell Biology, 35(8), 1263-1271.
http://dx.doi.org/10.1016/S1357-2725(03)00033-5
---------- CHICAGO ----------
Lombardo, M.E., Araujo, L.S., Batlle, A. "5-Aminolevulinic acid synthesis in epimastigotes of Trypanosoma cruzi" . International Journal of Biochemistry and Cell Biology 35, no. 8 (2003) : 1263-1271.
http://dx.doi.org/10.1016/S1357-2725(03)00033-5
---------- MLA ----------
Lombardo, M.E., Araujo, L.S., Batlle, A. "5-Aminolevulinic acid synthesis in epimastigotes of Trypanosoma cruzi" . International Journal of Biochemistry and Cell Biology, vol. 35, no. 8, 2003, pp. 1263-1271.
http://dx.doi.org/10.1016/S1357-2725(03)00033-5
---------- VANCOUVER ----------
Lombardo, M.E., Araujo, L.S., Batlle, A. 5-Aminolevulinic acid synthesis in epimastigotes of Trypanosoma cruzi. Int. J. Biochem. Cell Biol. 2003;35(8):1263-1271.
http://dx.doi.org/10.1016/S1357-2725(03)00033-5