Artículo

Cavagnari, B.M.; Tatián, M.; Sahade, R.J.; Esnal, G.B.; Santomé, J.A. "A fatty acid-binding protein and a protein disulphide isomerase-related protein expressed in urochordate gonad cytosol" (2000) International Journal of Biochemistry and Cell Biology. 32(7):769-777
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Abstract:

Despite the evolutionary-tree data suggesting that gene duplication leading to the divergence of the three branches which heart, liver and intestinal fatty acid-binding proteins belong to must have occurred before the vertebrate/invertebrate split, only the heart fatty acid-binding protein has been reported for invertebrates. In an attempt to shed light on this apparent inconsistency the presence of the other two branch members was investigated in the Urochordata Molgula pedunculata, an ascidian species close to vertebrates. The mantle-, gonad- and digestive tube-cytosolic fractions, obtained by centrifugation at 106,000 g, were incubated separately with [1-14C]palmitic acid and then fractionated on a Sephadex G-75 column. In the case of gonads and digestive tube, radioactive peaks corresponding to a molecular mass of 14-16 kDa, characteristic of fatty acid-binding proteins, were detected. When the experiment was performed on the mantle, this peak showing fatty acid binding capacity was absent. Western Blot of the radioactive 14-16 kDa Sephadex fraction from the urochordate gonad cross- reacted with rat liver fatty acid-binding protein anti-serum but did not do so with anti-rat intestinal, adipocyte or heart fatty acid-binding protein antisera. The material from the digestive tube was not recognized by any of the antisera. The most abundant protein in said 14-16 kDa fraction was a protein disulphide isomerase-related protein. Its partial amino acid sequence was determined. (C) 2000 Elsevier Science Ltd.

Registro:

Documento: Artículo
Título:A fatty acid-binding protein and a protein disulphide isomerase-related protein expressed in urochordate gonad cytosol
Autor:Cavagnari, B.M.; Tatián, M.; Sahade, R.J.; Esnal, G.B.; Santomé, J.A.
Filiación:Inst. Quim. y Fisicoquimica B., Fac. de Farmacia y Bioquímica, UBA-CONICET, Junin 956, 1113 Buenos Aires, Argentina
Depto. de Cs. Biológicas, Facultad de Cs. Exactas y Naturales, UBA-CONICET, 1428 Buenas Aires, Argentina
Palabras clave:Fatty acid-binding proteins; Molgula pedunculata; Protein disulphide isomerase; Sequence comparison; Urochordates; actin binding protein; fatty acid binding protein; palmitic acid; protein disulfide isomerase; adipocyte; amino acid sequence; article; cytosol; enzyme activity; gonad; nonhuman; protein analysis; protein binding; protein expression; species differentiation; Amino Acid Sequence; Animals; Cytosol; Fatty Acid-Binding Proteins; Gastrointestinal Tract; Gonads; Humans; Molecular Sequence Data; Protein Disulfide-Isomerases; Sequence Alignment; Urochordata; Ascidia; Invertebrata; Molgula pedunculata; Pedunculata; Urochordata; Vertebrata
Año:2000
Volumen:32
Número:7
Página de inicio:769
Página de fin:777
DOI: http://dx.doi.org/10.1016/S1357-2725(00)00014-5
Título revista:International Journal of Biochemistry and Cell Biology
Título revista abreviado:Int. J. Biochem. Cell Biol.
ISSN:13572725
CODEN:IJBBF
CAS:palmitic acid, 57-10-3; protein disulfide isomerase, 37318-49-3; Fatty Acid-Binding Proteins; Protein Disulfide-Isomerases, EC 5.3.4.1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13572725_v32_n7_p769_Cavagnari

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Citas:

---------- APA ----------
Cavagnari, B.M., Tatián, M., Sahade, R.J., Esnal, G.B. & Santomé, J.A. (2000) . A fatty acid-binding protein and a protein disulphide isomerase-related protein expressed in urochordate gonad cytosol. International Journal of Biochemistry and Cell Biology, 32(7), 769-777.
http://dx.doi.org/10.1016/S1357-2725(00)00014-5
---------- CHICAGO ----------
Cavagnari, B.M., Tatián, M., Sahade, R.J., Esnal, G.B., Santomé, J.A. "A fatty acid-binding protein and a protein disulphide isomerase-related protein expressed in urochordate gonad cytosol" . International Journal of Biochemistry and Cell Biology 32, no. 7 (2000) : 769-777.
http://dx.doi.org/10.1016/S1357-2725(00)00014-5
---------- MLA ----------
Cavagnari, B.M., Tatián, M., Sahade, R.J., Esnal, G.B., Santomé, J.A. "A fatty acid-binding protein and a protein disulphide isomerase-related protein expressed in urochordate gonad cytosol" . International Journal of Biochemistry and Cell Biology, vol. 32, no. 7, 2000, pp. 769-777.
http://dx.doi.org/10.1016/S1357-2725(00)00014-5
---------- VANCOUVER ----------
Cavagnari, B.M., Tatián, M., Sahade, R.J., Esnal, G.B., Santomé, J.A. A fatty acid-binding protein and a protein disulphide isomerase-related protein expressed in urochordate gonad cytosol. Int. J. Biochem. Cell Biol. 2000;32(7):769-777.
http://dx.doi.org/10.1016/S1357-2725(00)00014-5