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Bermúdez Moretti, M.; Correa García, S.R.; Chianelli, M.S.; Ramos, E.H.; Mattoon, J.R.; Batlle, A. "Evidence that 4-aminobutyric acid and 5-aminolevulinic acid share a common transport system into Saccharomyces cerevisiae" (1995) International Journal of Biochemistry and Cell Biology. 27(2):169-173
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Abstract:

It has been previously reported that 5-aminolevulinic acid (ALA) and 4-aminobutyric acid (GABA) share a common permease in Saccharomyces cerevisiae (Bermúdez Moretti et al., 1993). The aim of the present work was to determine the relationship between the transport of these compounds in isolated cells. Assessment of amino acid incorporation was performed in S. cerevisiae using 14C-ALA or 3H-GABA. Initial rates of ALA incorporation in cells grown in the presence of 5 mM ALA and 5 mM GABA, were three to four times lower than in cells grown without supplements. Kinetic studies indicate that GABA competitively inhibits ALA transport. During the growth phase GABA uptake was also inhibited by 74% and 60% in the presence of ALA and GABA, respectively. These findings indicate that in S. cerevisiae the structurally related compounds, ALA and GABA, may be incorporated into the cells by a common carrier protein. Should this occur in other lukaryotic cells it may explain the neurotoxic effect attributed to ALA in the pathogenesis of acute porphyrias. © 1995.

Registro:

Documento: Artículo
Título:Evidence that 4-aminobutyric acid and 5-aminolevulinic acid share a common transport system into Saccharomyces cerevisiae
Autor:Bermúdez Moretti, M.; Correa García, S.R.; Chianelli, M.S.; Ramos, E.H.; Mattoon, J.R.; Batlle, A.
Filiación:Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP) (CONICET, F.C.E.y N., UBA), Ciudad Universitaria, Pabellón II, 2o Piso, (1428) Buenos Aires, Argentine
Departamento de Bioquimica (CONICET, Facultad de Medicina, UBA), Paraguay 2155, 5o Piso, (1121) Buenos Aires, Argentine
Biotechnology Center, University of Colorado, Colorado Springs, United States
Palabras clave:4-Aminobutyric acid; 5-Aminolevulinic acid; Membrane transport; Permease; Saccharomyces cerevisiae; 4 aminobutyric acid; aminolevulinic acid; amino acid transport; article; isotope labeling; saccharomyces cerevisiae; Aminolevulinic Acid; Biological Transport; Cell Membrane; Culture Media; gamma-Aminobutyric Acid; Kinetics; Membrane Transport Proteins; Saccharomyces cerevisiae; Support, Non-U.S. Gov't; Time Factors
Año:1995
Volumen:27
Número:2
Página de inicio:169
Página de fin:173
DOI: http://dx.doi.org/10.1016/1357-2725(95)00002-7
Título revista:International Journal of Biochemistry and Cell Biology
Título revista abreviado:Int. J. Biochem. Cell Biol.
ISSN:13572725
CODEN:IJBBF
CAS:4 aminobutyric acid, 28805-76-7, 56-12-2; aminolevulinic acid, 106-60-5; Aminolevulinic Acid, 106-60-5; Culture Media; gamma-Aminobutyric Acid, 56-12-2; Membrane Transport Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13572725_v27_n2_p169_BermudezMoretti

Referencias:

  • Bermúdez Moretti, Correa Garcia, Stella, Ramos, Battle, δ-Aminolevulinic acid transport in Saccharomyces cerevisiae (1993) Int. J. Biochem., 25, pp. 1917-1924
  • Brennan, Cantrill, Kramer, Effect of δ-aminolaevulinic acid on GABA receptor binding in synaptic plasma membranes (1980) Int. J. Biochem., 12, pp. 833-835
  • Cooper, Metabolism and gene expression (1982) The Molecular Biology of the Yeast Saccharomyces cerevisiae, pp. 399-461. , J.N. Strathern, E.W. Jones, J.R. Broach, Cold Spring Harbor, New York
  • Grenson, Muyldermans, Broman, Vissers, 4-Aminobutyric acid (GABA) uptake in Baker's yeast Saccharomyces cerevisiae is mediated by the general amino-acid permease, the proline permease and a GABA-specific permease integrated into the GABA-catabolic pathway (1987) Biochem. (Life Sci. Adv.), 6, pp. 35-39
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  • McKelvey, Rai, Cooper, GABA transport in Saccharomyces cerevisiae (1990) Yeast, 6, pp. 263-270
  • Muller, Snyder, δ-Aminolaevulinic acid: influences on synaptic GABA receptor binding may explain CNS symptoms of porphyria (1977) Ann. Neurol., 2, pp. 340-342
  • Percy, Lamm, Taljaard, δ-Aminolaevulinic acid uptake, toxicity and effect on 14C-5-aminobutyric acid uptake into neurons and glia in culture (1981) J. Neurochem., 36, pp. 69-76
  • Ramos, El Guezzar, Grenson, Wiame, Mutations affecting the enzymes involved in the utilization of 4-aminobutyric acid as nitrogen source by the yeast Saccharomyces cerevisiae (1985) Eur. J. Biochem., 149, pp. 401-404
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  • Vissers, Andre, Muyldermans, Grenson, Induction of the 4-aminobutyrate and urea-catabolic pathways in Saccharomyces cerevisiae (1990) Eur. J. Biochem., 187, pp. 611-616
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Citas:

---------- APA ----------
Bermúdez Moretti, M., Correa García, S.R., Chianelli, M.S., Ramos, E.H., Mattoon, J.R. & Batlle, A. (1995) . Evidence that 4-aminobutyric acid and 5-aminolevulinic acid share a common transport system into Saccharomyces cerevisiae. International Journal of Biochemistry and Cell Biology, 27(2), 169-173.
http://dx.doi.org/10.1016/1357-2725(95)00002-7
---------- CHICAGO ----------
Bermúdez Moretti, M., Correa García, S.R., Chianelli, M.S., Ramos, E.H., Mattoon, J.R., Batlle, A. "Evidence that 4-aminobutyric acid and 5-aminolevulinic acid share a common transport system into Saccharomyces cerevisiae" . International Journal of Biochemistry and Cell Biology 27, no. 2 (1995) : 169-173.
http://dx.doi.org/10.1016/1357-2725(95)00002-7
---------- MLA ----------
Bermúdez Moretti, M., Correa García, S.R., Chianelli, M.S., Ramos, E.H., Mattoon, J.R., Batlle, A. "Evidence that 4-aminobutyric acid and 5-aminolevulinic acid share a common transport system into Saccharomyces cerevisiae" . International Journal of Biochemistry and Cell Biology, vol. 27, no. 2, 1995, pp. 169-173.
http://dx.doi.org/10.1016/1357-2725(95)00002-7
---------- VANCOUVER ----------
Bermúdez Moretti, M., Correa García, S.R., Chianelli, M.S., Ramos, E.H., Mattoon, J.R., Batlle, A. Evidence that 4-aminobutyric acid and 5-aminolevulinic acid share a common transport system into Saccharomyces cerevisiae. Int. J. Biochem. Cell Biol. 1995;27(2):169-173.
http://dx.doi.org/10.1016/1357-2725(95)00002-7