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Mair, G.; Shi, H.; Li, H.; Djikeng, A.; Aviles, H.O.; Bishop, J.R.; Falcone, F.H.; Gavrilescu, C.; Montgomery, J.L.; Santori, M.I.; Stern, L.S.; Wang, Z.; Ullu, E.; Tschudi, C. "A new twist in trypanosome RNA metabolism: Cis-splicing of pre-mRNA" (2000) RNA. 6(2):163-169
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Abstract:

It has been known for almost a decade and a half that in trypanosomes all mRNAs are trans-spliced by addition to the 5' end of the spliced leader (SL) sequence. During the same time period the conviction developed that classical cis-splicing introns are not present in the trypanosome genome and that the trypanosome gene arrangement is highly compact with small intergenic regions separating one gene from the next. We have now discovered that these tenets are no longer true. Poly(A) polymerase (PAP) genes in Trypanosoma brucei and Trypanosoma cruzi are split by intervening sequences of 653 and 302 nt, respectively. The intervening sequences occur at identical positions in both organisms and obey the GT/AG rule of cis-splicing introns. PAP mRNAs are trans-spliced at the very 5' end as well as internally at the 3' splice site of the intervening sequence. Interestingly, 11 nucleotide positions past the actual 5' splice site are conserved between the T. brucei and T. cruzi introns. Point mutations in these conserved positions, as well as in the AG dinucleotide of the 3' splice site, abolish intron removal in vivo. Our results, together with the recent discovery of cis-splicing introns in Euglena gracilis, suggest that both trans- and cis-splicing are ancient acquisitions of the eukaryotic cell.

Registro:

Documento: Artículo
Título:A new twist in trypanosome RNA metabolism: Cis-splicing of pre-mRNA
Autor:Mair, G.; Shi, H.; Li, H.; Djikeng, A.; Aviles, H.O.; Bishop, J.R.; Falcone, F.H.; Gavrilescu, C.; Montgomery, J.L.; Santori, M.I.; Stern, L.S.; Wang, Z.; Ullu, E.; Tschudi, C.
Filiación:Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520-8022, United States
Indiana State University, Life Sciences Department, Terre-Haute, IN 47809, United States
Dept. of Biochem. and Molec. Genet., University of Alabama at Birmingham, Birmingham, AL 35294, United States
Inst. Cell, Anim. and Pop. Biology, University of Edinburgh, Edinburgh EH9 3JT, United Kingdom
Dept. of Microbiology and Immunology, Cornell University, Ithaca, NY 14850, United States
Walter and Eliza Hall Institute, Post Office, Royal Melbourne Hospital, North Melbourne 3050, Australia
Inst. Invest. Ing. Genet. y Biol. M., Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
113B Vet. Admin. Medical Center, San Francisco, CA 94121, United States
Department of Biological Chemistry, Johns Hopkins University, Baltimore, MD 21205, United States
Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520-8022, United States
Department of Internal Medicine, LCI 805, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520-8022, United States
Palabras clave:Introns; Kinetoplastidae; Poly(A) polymerase; Trans-splicing; Trypanosoma brucei; Trypanosoma cruzi; amino acid; messenger RNA; polynucleotide adenylyltransferase; protozoal RNA; spliced leader RNA; amino acid sequence; article; Euglena gracilis; gene isolation; intron; nucleotide sequence; point mutation; priority journal; RNA analysis; RNA metabolism; structural gene; Trypanosoma; Trypanosoma brucei; Trypanosoma cruzi; Amino Acid Sequence; Animals; Base Sequence; Conserved Sequence; DNA Primers; DNA, Protozoan; Exons; Genes, Protozoan; Introns; Molecular Sequence Data; Phylogeny; Point Mutation; Polynucleotide Adenylyltransferase; RNA Precursors; RNA Splicing; RNA, Protozoan; Trypanosoma brucei brucei; Trypanosoma cruzi; Euglena gracilis; Eukaryota; Protozoa; Trypanosoma; Trypanosoma brucei; Trypanosoma cruzi
Año:2000
Volumen:6
Número:2
Página de inicio:163
Página de fin:169
DOI: http://dx.doi.org/10.1017/S135583820099229X
Título revista:RNA
Título revista abreviado:RNA
ISSN:13558382
CODEN:RNARF
CAS:DNA Primers; DNA, Protozoan; Polynucleotide Adenylyltransferase, EC 2.7.7.19; RNA Precursors; RNA, Protozoan
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13558382_v6_n2_p163_Mair

Referencias:

  • Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller, W., Lipman, D.J., Gapped BLAST and PSI-BLAST: A new generation of protein database search programs (1997) Nucleic Acids Res, 25, pp. 3389-3402
  • Ares M., Jr., Weiser, B., Rearrangement of snRNA structure during assembly and function of the spliceosome (1995) Prog Nucleic Acid Res Mol Biol, 50, pp. 131-159
  • Boothroyd, J.C., Cross, G.A., Transcripts coding for variant surface glycoproteins of Trypanosoma brucei have a short, identical exon at their 5′ end (1982) Gene, 20, pp. 281-289
  • Breckenridge, D.G., Watanabe, Y., Greenwood, S.J., Gray, M.W., Schnare, M.N., U1 small nuclear RNA and spliceosomal introns in Euglena gracilis (1999) Proc Natl Acad Sci USA, 96, pp. 852-856
  • Conrad, R., Liou, R.F., Blumenthal, T., Conversion of a trans-spliced C. elegans gene into a conventional gene by introduction of a splice donor site (1993) EMBO J, 72, pp. 1249-1255
  • Fantoni, A., Dare, A.O., Tschudi, C., RNA polymerase III-mediated transcription of the trypanosome U2 small nuclear RNA gene is controlled by both intragenic and extragenic regulatory elements (1994) Mol Cell Biol, 14, pp. 2021-2028
  • Hall, S.L., Padgett, R.A., Conserved sequences in a class of rare eukaryotic nuclear introns with non-consensus splice sites (1994) J Mol Biol, 239, pp. 357-365
  • Hannon, G.J., Maroney, P.A., Nilsen, T.W., U small nuclear ribonucleoprotein requirements for nematode cis- and trans-splicing in vitro (1991) J Biol Chem, 266, pp. 22792-22795
  • Krause, M., Hirsh, D., A trans-spliced leader sequence on actin mRNA in C. elegans (1987) Cell, 49, pp. 753-761
  • Laird, P.W., Zomerdijk, J.C., De Korte, D., Borst, P., In vivo labelling of intermediates in the discontinuous synthesis of mRNAs in Trypanosoma brucei (1987) EMBO J, 6, pp. 1055-1062
  • Maroney, P.A., Yu, Y.T., Jankowska, M., Nilsen, T.W., Direct analysis of nematode cis- and trans-spliceosomes: A functional role for U5 snRNA in spliced leader addition trans-splicing and the identification of novel Sm snRNPs (1996) RNA, 2, pp. 735-745
  • Martin, G., Keller, W., Mutational analysis of mammalian poly(A) polymerase identifies a region for primer binding and catalytic domain, homologous to the family X polymerases, and to other nucleotidyltransferases (1996) EMBO J, 15, pp. 2593-2603
  • Matthews, K.R., Tschudi, C., Ullu, E., A common pyrimidine-rich motif governs trans-splicing and polyadenylation of tubulin polycistronic pre-mRNA in trypanosomes (1994) Genes & Dev, 8, pp. 491-501
  • Mottram, J., Perry, K.L., Lizardi, P.M., Luhrmann, R., Agabian, N., Nelson, R.G., Isolation and sequence of four small nuclear U RNA genes of Trypanosoma brucei subsp. Brucei: Identification of the U2, U4, and U6 RNA analogs (1989) Mol Cell Biol, 9, pp. 1212-1223
  • Murphy, W.J., Watkins, K.P., Agabian, N., Identification of a novel Y branch structure as an intermediate in trypanosome mRNA processing: Evidence for trans splicing (1986) Cell, 47, pp. 517-525
  • Nilsen, T.W., trans-splicing of nematode premessenger RNA (1993) Annu Rev Microbiol, 47, pp. 413-440
  • Raabe, T., Bollum, F.J., Manley, J.L., Primary structure and expression of bovine poly(A) polymerase (1991) Nature, 353, pp. 229-234
  • Rajkovic, A., Davis, R.E., Simonsen, J.N., Rottman, F.M., A spliced leader is present on a subset of mRNAs from the human parasite Schistosoma mansoni (1990) Proc Natl Acad Sci USA, 87, pp. 8879-8883
  • Schnare, M.N., Gray, M.W., A candidate U1 small nuclear RNA for trypanosomatid protozoa (1999) J Biol Chem, 274, pp. 23691-23694
  • Silva, E., Ullu, E., Kobayashi, R., Tschudi, C., Trypanosome capping enzymes display a novel two-domain structure (1998) Mol Cell Biol, 18, pp. 4612-4619
  • Sutton, R.E., Boothroyd, J.C., Evidence for trans splicing in trypanosomes (1986) Cell, 47, pp. 527-535
  • Tessier, L.H., Keller, M., Chan, R.L., Fournier, R., Weil, J.H., Imbault, P., Short leader sequences may be transferred from small RNAs to pre-mature mRNAs by trans-splicing in Euglena (1991) EMBO J, 10, pp. 2621-2625
  • Tschudi, C., Ullu, E., Destruction of U2, U4, or U6 small nuclear RNA blocks trans splicing in trypanosome cells (1990) Cell, 67, pp. 459-466
  • Ullu, E., Tschudi, C., Gunzl, A., trans-splicing in trypanosomatid protozoa (1996) Molecular Biology of Parasitic Protozoa, pp. 115-133. , Smith DF, Parsons M, eds. Oxford, United Kingdom: Oxford University Press
  • Wirtz, E., Leal, S., Ochatt, C., Cross, G.A., A tightly regulated inducible expression system for conditional gene knock-outs and dominant-negative genetics in Trypanosoma brucei (1999) Mol Biochem Parasitol, 99, pp. 89-101

Citas:

---------- APA ----------
Mair, G., Shi, H., Li, H., Djikeng, A., Aviles, H.O., Bishop, J.R., Falcone, F.H.,..., Tschudi, C. (2000) . A new twist in trypanosome RNA metabolism: Cis-splicing of pre-mRNA. RNA, 6(2), 163-169.
http://dx.doi.org/10.1017/S135583820099229X
---------- CHICAGO ----------
Mair, G., Shi, H., Li, H., Djikeng, A., Aviles, H.O., Bishop, J.R., et al. "A new twist in trypanosome RNA metabolism: Cis-splicing of pre-mRNA" . RNA 6, no. 2 (2000) : 163-169.
http://dx.doi.org/10.1017/S135583820099229X
---------- MLA ----------
Mair, G., Shi, H., Li, H., Djikeng, A., Aviles, H.O., Bishop, J.R., et al. "A new twist in trypanosome RNA metabolism: Cis-splicing of pre-mRNA" . RNA, vol. 6, no. 2, 2000, pp. 163-169.
http://dx.doi.org/10.1017/S135583820099229X
---------- VANCOUVER ----------
Mair, G., Shi, H., Li, H., Djikeng, A., Aviles, H.O., Bishop, J.R., et al. A new twist in trypanosome RNA metabolism: Cis-splicing of pre-mRNA. RNA. 2000;6(2):163-169.
http://dx.doi.org/10.1017/S135583820099229X