Artículo
Abstract:
The aim of the present study was to investigate the regulation of the in vitro DNA synthesis of ovarian cells recovered from prepubertal rats 48 h after administration of pregnant mare's serum gonadotrophin alone (granulosa cells) or followed by human chorionic gonadotrophin (luteal cells). Isolated granulosa cells were cultured in serum-free medium, different stimuli added for periods of 48 h, and 3H-thymidine incorporation was measured. Both follicle-stimulating hormone (FSH) and luteinizing hormone (LH) inhibited 3H-thymidine incorporation by cultured granulosa cells in a dose-dependent manner (FSH: 10, 100, 200 ng/mL = 26, 41, 49% inhibition, respectively; LH: 0.1, 1, 10 ng/mL = 11, 37, 75% inhibition, respectively). On the other hand, estradiol was found to stimulate 3H-thymidine incorporation in granulosa cells (Estradiol: 5, 50, 500 ng/mL = 17, 37, 76% stimulation, respectively). In luteal cells, the rate of basal 3H-thymidine incorporation was very low (granulosa cells: 2560 ± 310; luteal cells: 661 ± 92 cpm/100,000 cells) and not modified by any stimulus. To determine the possible production of an inhibitory growth factor by the early corpus luteum, 3H-thymidine incorporation by granulosa cells was assessed in the presence of 10% conditioned media (CM) recovered from luteal cell cultures. A marked inhibition both in basal and estradiol-stimulated 3H-thymidine incorporation was observed (74 and 76% of inhibition, respectively). Results suggest that an inhibitory growth factor produced by luteal cells after luteinizing gonadotrophin stimulus could be involved in the differentiation of growing follicles to corpus luteum.
Registro:
| Documento: | Artículo |
| Título: | Regulation of ovarian follicle differentiation in gonadotrophin-stimulated rats |
| Autor: | Andreu, C.V.; Buscaglia, C.A.; Parborell, F.; Stein, P.; Tesone, M. |
| Filiación: | Inst. de Biol. y Med. Experimental, CONICET, Buenos Aires, Argentina Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina Inst. de Biol. y Med. Experimental, Obligado 2490(1428), Buenos Aires, Argentina |
| Palabras clave: | Corpus luteum; Follicle differentiation; Granulosa cell; Ovary; chorionic gonadotropin; estradiol; follitropin; gonadotropin; luteinizing hormone; novormon; seric gonadotropin; thymidine; unclassified drug; animal cell; animal experiment; article; controlled study; corpus luteum; female; granulosa cell; luteal cell; nonhuman; ovary follicle; priority journal; rat; Animals; Cell Differentiation; Cells, Cultured; Corpus Luteum; Culture Media, Conditioned; DNA; Female; Gonadotropins; Gonadotropins, Equine; Granulosa Cells; Ovarian Follicle; Ovary; Pregnancy; Rats; Rats, Sprague-Dawley; Steroids; Stimulation, Chemical; Thymidine |
| Año: | 1997 |
| Volumen: | 6 |
| Número: | 2 |
| Página de inicio: | 145 |
| Página de fin: | 151 |
| Título revista: | Endocrine |
| Título revista abreviado: | ENDOCRINE |
| ISSN: | 1355008X |
| CODEN: | EOCRE |
| CAS: | chorionic gonadotropin, 9002-61-3; estradiol, 50-28-2; follitropin, 9002-68-0; gonadotropin, 63231-54-9; luteinizing hormone, 39341-83-8, 9002-67-9; seric gonadotropin, 9002-70-4; thymidine, 50-89-5; Culture Media, Conditioned; DNA, 9007-49-2; Gonadotropins; Gonadotropins, Equine; Steroids; Thymidine, 50-89-5 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1355008X_v6_n2_p145_Andreu |
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Citas:
---------- APA ----------
Andreu, C.V., Buscaglia, C.A., Parborell, F., Stein, P. & Tesone, M. (1997) . Regulation of ovarian follicle differentiation in gonadotrophin-stimulated rats. Endocrine, 6(2), 145-151.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1355008X_v6_n2_p145_Andreu [ ]
---------- CHICAGO ----------
Andreu, C.V., Buscaglia, C.A., Parborell, F., Stein, P., Tesone, M. "Regulation of ovarian follicle differentiation in gonadotrophin-stimulated rats" . Endocrine 6, no. 2 (1997) : 145-151.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1355008X_v6_n2_p145_Andreu [ ]
---------- MLA ----------
Andreu, C.V., Buscaglia, C.A., Parborell, F., Stein, P., Tesone, M. "Regulation of ovarian follicle differentiation in gonadotrophin-stimulated rats" . Endocrine, vol. 6, no. 2, 1997, pp. 145-151.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1355008X_v6_n2_p145_Andreu [ ]
---------- VANCOUVER ----------
Andreu, C.V., Buscaglia, C.A., Parborell, F., Stein, P., Tesone, M. Regulation of ovarian follicle differentiation in gonadotrophin-stimulated rats. ENDOCRINE. 1997;6(2):145-151.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1355008X_v6_n2_p145_Andreu [ ]
