Artículo

Este artículo es de Acceso Abierto y puede ser descargado en su versión final desde nuestro repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Curcumin (diferuloylmethane) is the active ingredient of the spice plant Curcuma longa and has been shown to act anti-tumorigenic in different types of tumours. Therefore, we have studied its effect in pituitary tumour cell lines and adenomas. Proliferation of lactosomatotroph GH3 and somatotroph MtT/S rat pituitary cells as well as of corticotroph AtT20 mouse pituitary cells was inhibited by curcumin in monolayer cell culture and in colony formation assay in soft agar. Fluorescence-activated cell sorting (FACS) analysis demonstrated curcumin-induced cell cycle arrest at G2/M. Analysis of cell cycle proteins by immunoblotting showed reduction in cyclin D1, cyclin-dependent kinase 4 and no change in p27kip. FACS analysis with Annexin V-FITC/7-aminoactinomycin D staining demonstrated curcumin-induced early apoptosis after 3, 6, 12 and 24 h treatment and nearly no necrosis. Induction of DNA fragmentation, reduction of Bcl-2 and enhancement of cleaved caspase-3 further confirmed induction of apoptosis by curcumin. Growth of GH3 tumours in athymic nude mice was suppressed by curcumin in vivo. In endocrine pituitary tumour cell lines, GH, ACTH and prolactin production were inhibited by curcumin. Studies in 25 human pituitary adenoma cell cultures have confirmed the antitumorigenic and hormone-suppressive effects of curcumin. Altogether, the results described in this report suggest this natural compound as a good candidate for therapeutic use on pituitary tumours. © 2009 Society for Endocrinology Printed in Great Britain.

Registro:

Documento: Artículo
Título:Curcumin acts as anti-tumorigenic and hormone-suppressive agent in murine and human pituitary tumour cells in vitro and in vivo
Autor:Schaaf, C.; Shan, B.; Buchfelder, M.; Losa, M.; Kreutzer, J.; Rachinger, W.; Stalla, G.K.; Schilling, T.; Arzt, E.; Perone, M.J.; Renner, U.
Filiación:Neuroendocrinology Group, Max Planck Institute of Psychiatry, Kraepelinstrasse 10, D-80804 Munich, Germany
Neurosurgical Clinic, University of Erlangen-Nuremburg, Erlangen, Germany
Department of Neurosurgery, Istituto San Raffaele, Milano, Italy
Neurosurgical Clinic, Technical University of Munich, Munich, Germany
Neurosurgical Clinic, University of Munich, Munich, Germany
Department of Internal Medicine 1 and Clinical Chemistry, University of Heidelberg, Heidelberg, Germany
Departamento de Fisiología Y Biología Molecular Y Celular, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Pabellon II-2 Piso, C1428 Buenos Aires, Argentina
IFIBYNE-CONICET, Ciudad Universitaria, Pabellon II-2 Piso, C1428 Buenos Aires, Argentina
Palabras clave:7 aminodactinomycin; caspase 3; cell cycle protein; curcumin; cyclin D1; cyclin dependent kinase 4; cyclin dependent kinase inhibitor 1B; lipocortin 5; protein bcl 2; antineoplastic activity; apoptosis; article; cancer cell culture; cancer inhibition; cell cycle arrest; cell cycle G2 phase; cell cycle M phase; cell proliferation; cell strain GH3; clinical article; colony formation; controlled study; Curcuma longa; DNA fragmentation; fluorescence activated cell sorting; human; human cell; hypophysis cell; hypophysis tumor; immunoblotting; in vitro study; in vivo study; mouse; nonhuman; nude mouse; Animals; Antineoplastic Agents; Apoptosis; Blotting, Western; Caspase 3; Cell Cycle; Cell Line, Tumor; Cell Proliferation; Curcumin; Cyclin D1; Cyclin-Dependent Kinase 4; Flow Cytometry; Humans; Male; Mice; Mice, Nude; Pituitary Hormones; Pituitary Neoplasms; Rats
Año:2009
Volumen:16
Número:4
Página de inicio:1339
Página de fin:1350
DOI: http://dx.doi.org/10.1677/ERC-09-0129
Título revista:Endocrine-Related Cancer
Título revista abreviado:Endocr.-Relat. Cancer
ISSN:13510088
CODEN:ERCAE
CAS:7 aminodactinomycin, 7240-37-1; caspase 3, 169592-56-7; curcumin, 458-37-7; cyclin dependent kinase 4, 147014-97-9; lipocortin 5, 111237-10-6; protein bcl 2, 219306-68-0; Antineoplastic Agents; Caspase 3, 3.4.22.-; Curcumin, 458-37-7; Cyclin D1, 136601-57-5; Cyclin-Dependent Kinase 4, 2.7.11.22; Pituitary Hormones
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_13510088_v16_n4_p1339_Schaaf.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13510088_v16_n4_p1339_Schaaf

Referencias:

  • Aggarwal, B.B., Sundaram, C., Malani, N., Ichikawa, H., Curcumin: The Indian solid gold (2007) Advances in Experimental Medicine and Biology, 595, pp. 1-75
  • Anand, P., Kunnumakkara, A.B., Newman, R.A., Aggarwal, B.B., Bioavailability of curcumin: Problems and promises (2007) Molecular Pharmacology, 4, pp. 807-818
  • Barnes, P.J., Role of HDAC2 in the pathophysiology of COPD (2008) Annual Reviews in Physiology, 71, pp. 451-464
  • Bilodeau, S., Vallette-Kasic, S., Gauthier, Y., Figarella-Branger, D., Brue, T., Berthelet, F., Lacroix, A., Drouin, J., Role of Brg1 and HDAC2 in GR trans-repression of the pituitary POMC gene and misexpression in Cushing disease (2006) Genes and Development, 20 (20), pp. 2871-2886. , http://www.genesdev.org/cgi/reprint/20/20/2871, DOI 10.1101/gad.1444606
  • Bisht, S., Feldmann, G., Soni, S., Ravi, R., Karikar, C., Maitra, A., Polymeric nanoparticle-encapsulated curcumin ("nanocurcumin"): A novel strategy for human cancer therapy (2007) Journal of Nanobiotechnology, 5, pp. 1-18
  • Cao, J., Liu, Y., Jia, L., Zhou, H.-M., Kong, Y., Yang, G., Jiang, L.-P., Zhong, L.-F., Curcumin induces apoptosis through mitochondrial hyperpolarization and mtDNA damage in human hepatoma G2 cells (2007) Free Radical Biology and Medicine, 43 (6), pp. 968-975. , DOI 10.1016/j.freeradbiomed.2007.06.006, PII S0891584907003917
  • Carroll, C.E., Ellersieck, M.R., Hyder, S.M., Curcumin inhibits MPA-induced secretion of VEGF from T47-D human breast cancer cells (2008) Menopause, 15, pp. 570-574
  • Chadalapaka, G., Jutooru, I., Chintharlapalli, S., Papineni, S., Smith III, R., Li, X., Safe, S., Curcumin decreases specificity protein expression in bladder cancer cells (2008) Cancer Research, 68, pp. 5345-5354
  • Chanson, P., Salenave, S., Acromegaly (2008) Orphanet Journal of Rare Diseases, 3, p. 17
  • Corson, T.W., Crews, C.M., Molecular understanding and modern application of traditional medicines: Triumphs and trials (2007) Cell, 130, pp. 769-774
  • Davis, J.R., Farrell, W.E., Clayton, R.N., Pituitary tumours (2001) Reproduction, 121, pp. 363-371
  • Dhillon, N., Aggarwal, B.B., Newman, R.A., Wolff, R.A., Kunnumakkara, A.B., Abbruzzese, J.L., Ng, C.S., Kurzrock, R., Phase II trial of curcumin in patients with advanced pancreatic cancer (2008) Clinical Cancer Research, 14, pp. 4491-4499
  • Dujic, J., Kippenberger, S., Ramirez-Bosca, A., Diaz-Alperi, J., Bereiter-Hahn, J., Kaufmann, R., Bernd, A., Hofmann, M., Curcumin in combination with visible light inhibits tumor growth in a xenograft tumor model (2008) International Journal of Cancer, 124, pp. 1422-1428
  • Ezzat, S., Asa, S.L., Mechanisms of disease: The pathogenesis of pituitary tumors (2006) Nature Clinical Practice. Endocrinology and Metabolism, 2, pp. 220-230
  • Fitzpatrick, F.A., Wheeler, R., The immunopharmacology of paclitaxel (Taxol), docetaxel (Taxotere), and related agents (2003) International Immunopharmacology, 3 (13-14), pp. 1699-1714. , DOI 10.1016/j.intimp.2003.08.007
  • Gidding, C.E., Kellie, S.J., Kamps, W.A., De Graaf, S.S., Vincristine revisited (1999) Critical Review in Oncology and Hematology, 29, pp. 267-287
  • Hatcher, H., Planalp, R., Cho, J., Torti, F.M., Torti, S.V., Curcumin: From ancient medicine to current clinical trials (2008) Cellular and Molecular Life Sciences, 65, pp. 1631-1652
  • Johnson, J.J., Mukhtar, H., Curcumin for chemoprevention of colon cancer (2007) Cancer Letters, 255, pp. 170-181
  • Kunnumakkara, A.B., Anand, P., Aggarwal, B.B., Curcumin inhibits proliferation, invasion, angiogenesis and metastasis of different cancers through interaction with multiple cell signaling proteins (2008) Cancer Letters, 269, pp. 199-225
  • Mancini, T., Casanueva, F.F., Giustina, A., Hyperprolactinemia and prolactinomas (2008) Endocrinology and Metabolism Clinics of North America, 37, pp. 67-99
  • Miller, M., Chen, S., Woodliff, J., Kansra, S., Curcumin (diferuloylmethane) inhibits cell proliferation, induces apoptosis, and decreases hormone levels and secretion in pituitary tumor cells (2008) Endocrinology, 149, pp. 4158-4167
  • Newell-Price, J., Bertagna, X., Grossman, A.B., Nieman, L.K., Cushing's syndrome (2006) Lancet, 367, pp. 1605-1617
  • Pae, H.O., Jeong, S.O., Kim, H.S., Kim, S.H., Song, Y.S., Kim, S.K., Chai, K.Y., Chung, H.T., Dimethoxycurcumin, a synthetic curcumin analogue with higher metabolic stability, inhibits NO production, inducible NO synthase expression and NF-kB activation in RAW264.7 macrophages activated with LPS (2008) Molecular Nutrition & Food Research, 52, pp. 1082-1091
  • Paez-Pereda, M., Giacomini, D., Refojo, D., Nagashima, A.C., Hopfner, U., Grubler, Y., Chervin, A., Arzt, E., Involvement of bone morphogenetic protein 4 (BMP-4) in pituitary prolactinoma pathogenesis through a Smad/estrogen receptor crosstalk (2003) Proceedings of the National Academy of Sciences of the United States of America, 100 (3), pp. 1034-1039. , DOI 10.1073/pnas.0237312100
  • Park, C., Kim, G.Y., Kim, G.D., Choi, B.T., Park, Y.M., Choi, Y.H., Induction of G2/M arrest and inhibition of cyclooxygenase-2 activity by curcumin in human bladder cancer T24 cells (2006) Oncology Reports, 15, pp. 1225-1231
  • Perez Castro, C., Giacomini, D., Carbia Nagashima, A., Onofri, C., Graciarena, M., Kobayashi, K., Paez-Pereda, M., Arzt, E., Reduced expression of the cytokine transducer gp130 inhibits hormone secretion, cell growth, and tumor development of pituitary lactosomatotrophic GH3 cells (2003) Endocrinology, 144 (2), pp. 693-700. , DOI 10.1210/en.2002-220891
  • Quinn, J., Fisher, P.W., Capocasale, R.J., Achuthanandam, R., Kam, M., Bugelski, P.J., Hrebien, L., A statistical pattern recognition approach for determining cellular viability and lineage phenotype in cultured cells and murine bone marrow (2007) Cytometry Part a, 71 (8), pp. 612-624. , DOI 10.1002/cyto.a.20416
  • Renner, U., Newton, C.J., Pagotto, U., Sauer, J., Arzt, E., Stalla, G.K., Involvement of interleukin-1 and interleukin-1 receptor antagonist in rat pituitary cell growth regulation (1995) Endocrinology, 136, pp. 3186-3193
  • Renner, U., Arzberger, T., Pagotto, U., Leimgruber, S., Uhl, E., Muller, A., Lange, M., Stalla, G.K., Heterogenous dopamine D2 receptor subtype messenger ribonucleic acid expression in clinically nonfunctioning pituitary adenomas (1998) Journal of Clinical Endocrinology and Metabolism, 83 (4), pp. 1368-1375. , DOI 10.1210/jc.83.4.1368
  • Sa, G., Das, T., Anti cancer effects of curcumin: Cycle of life and death (2008) Cell Division, 3, p. 14
  • Sandur, S.K., Ichikawa, H., Pandey, M.K., Kunnumakkara, A.B., Sung, B., Sethi, G., Aggarwal, B.B., Role of pro-oxidants and antioxidants in the anti-inflammatory and apoptotic effects of curcumin (diferuloylmethane) (2007) Free Radical Biology and Medicine, 43 (4), pp. 568-580. , DOI 10.1016/j.freeradbiomed.2007.05.009, PII S0891584907003255
  • Shankar, S., Ganapathy, S., Chen, Q., Srivastava, R.K., Curcumin sensitizes TRAIL-resistant xenografts: Molecular mechanisms of apoptosis, metastasis and angiogenesis (2008) Molecular Cancer, 7, p. 16. , DOI 10.1186/1476-4598-7-16
  • Shishodia, S., Chaturvedi, M.M., Aggarwal, B.B., Role of curcumin in cancer therapy (2007) Current Problems in Cancer, 31, pp. 243-305
  • Singh, S., From exotic spice to modern drug? (2007) Cell, 130, pp. 765-768
  • Srivastava, R.K., Chen, Q., Siddiqui, I., Sarva, K., Shankar, S., Linkage of curcumin-induced cell cycle arrest and apoptosis by cyclin-dependent kinase inhibitor p21(/WAF1/CIP1) (2007) Cell Cycle, 6, pp. 2953-2961
  • Stalla, G.K., Stalla, J., Von Werder, K., Muller, O.A., Gerzer, R., Hollt, V., Jakobs, K.H., Nitroimidazole derivatives inhibit anterior pituitary cell function apparently by a direct effect on the catalytic subunit of the adenylate cyclase holoenzyme (1989) Endocrinology, 125 (2), pp. 699-706

Citas:

---------- APA ----------
Schaaf, C., Shan, B., Buchfelder, M., Losa, M., Kreutzer, J., Rachinger, W., Stalla, G.K.,..., Renner, U. (2009) . Curcumin acts as anti-tumorigenic and hormone-suppressive agent in murine and human pituitary tumour cells in vitro and in vivo. Endocrine-Related Cancer, 16(4), 1339-1350.
http://dx.doi.org/10.1677/ERC-09-0129
---------- CHICAGO ----------
Schaaf, C., Shan, B., Buchfelder, M., Losa, M., Kreutzer, J., Rachinger, W., et al. "Curcumin acts as anti-tumorigenic and hormone-suppressive agent in murine and human pituitary tumour cells in vitro and in vivo" . Endocrine-Related Cancer 16, no. 4 (2009) : 1339-1350.
http://dx.doi.org/10.1677/ERC-09-0129
---------- MLA ----------
Schaaf, C., Shan, B., Buchfelder, M., Losa, M., Kreutzer, J., Rachinger, W., et al. "Curcumin acts as anti-tumorigenic and hormone-suppressive agent in murine and human pituitary tumour cells in vitro and in vivo" . Endocrine-Related Cancer, vol. 16, no. 4, 2009, pp. 1339-1350.
http://dx.doi.org/10.1677/ERC-09-0129
---------- VANCOUVER ----------
Schaaf, C., Shan, B., Buchfelder, M., Losa, M., Kreutzer, J., Rachinger, W., et al. Curcumin acts as anti-tumorigenic and hormone-suppressive agent in murine and human pituitary tumour cells in vitro and in vivo. Endocr.-Relat. Cancer. 2009;16(4):1339-1350.
http://dx.doi.org/10.1677/ERC-09-0129