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Objectives: Reports investigating the effects of antioxidants on obesity have provided contradictory results. We have previously demonstrated that treatment with the antioxidant N-acetylcysteine (NAC) inhibits cellular triglyceride (Tg) accumulation as well as total cellular monoamine oxidase A (MAOA) expression in 3T3-L1 mature adipocytes (Calzadilla et al., Redox Rep. 2013;210–218). Here we analyzed the role of NAC on adipogenic differentiation pathway. Methods: Assays were conducted using 3T3-L1 preadipocytes (undifferentiated cells: CC), which are capable of differentiating into mature adipocytes (differentiated cells: DC). We studied the effects of different doses of NAC (0.01 or 1 mM) on DC, to evaluate cellular expression of phospho-JNK½ (pJNK½), phospho-ERK½ (pERK½) and, mitochondrial expression of citrate synthase, fumarate hydratase and MAOA. Results: Following the differentiation of preadipocytes, an increase in the expression levels of pJNK½ and pERK½ was observed, together with mitotic clonal expansion (MCE). We found that both doses of NAC decreased the expression of pJNK½ and pERK½. Consistent with these results, NAC significantly inhibited MCE and modified the expression of different mitochondrial proteins. Discussion: Our results suggested that NAC could inhibit Tg and mitochondrial protein expression by preventing both MCE and kinase phosphorylation. © 2016 Informa UK Limited, trading as Taylor & Francis Group.


Documento: Artículo
Título:N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation
Autor:Soto, D.; Gomez-Serrano, M.; Pieralisi, A.; Calvo, J.C.; Peral, B.; Guerra, L.N.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires–IQUIBICEN, CONICET, Buenos Aires, Argentina
Instituto de Investigaciones Biomédicas Alberto Sols, Consejo Superior de Investigaciones Científicas (CSIC) y Universidad Autónoma de Madrid, (CSIC, UAM), Madrid, Spain
IBYME, CONICET, Buenos Aires, Argentina
Palabras clave:3T3-L1; adipocyte differentiation; Antioxidant; kinase phosphorylation; MAPK; mitocondria; monoamine oxidase A; obesity; acetylcysteine; amine oxidase (flavin containing) isoenzyme A; citrate synthase; fumarate hydratase; mitochondrial protein; mitogen activated protein kinase 1; mitogen activated protein kinase 3; mitogen activated protein kinase p38; stress activated protein kinase 1; acetylcysteine; amine oxidase (flavin containing); antioxidant; adipocyte; adipogenesis; Article; cell count; cell differentiation; citric acid cycle; controlled study; mitochondrion; mitosis; priority journal; proadipocyte; protein expression; protein phosphorylation; 3T3-L1 cell line; adipocyte; animal; cell differentiation; cytology; drug effect; metabolism; mouse; phosphorylation; 3T3-L1 Cells; Acetylcysteine; Adipocytes; Animals; Antioxidants; Cell Differentiation; Mice; Monoamine Oxidase; Phosphorylation
Página de inicio:265
Página de fin:271
Título revista:Redox Report
Título revista abreviado:Redox Rep.
CAS:acetylcysteine, 616-91-1; citrate synthase, 9027-96-7; fumarate hydratase, 9032-88-6; mitogen activated protein kinase 1, 137632-08-7; mitogen activated protein kinase 3, 137632-07-6; amine oxidase (flavin containing), 37255-42-8, 9001-66-5, 9059-11-4; Acetylcysteine; Antioxidants; Monoamine Oxidase


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---------- APA ----------
Soto, D., Gomez-Serrano, M., Pieralisi, A., Calvo, J.C., Peral, B. & Guerra, L.N. (2017) . N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation. Redox Report, 22(6), 265-271.
---------- CHICAGO ----------
Soto, D., Gomez-Serrano, M., Pieralisi, A., Calvo, J.C., Peral, B., Guerra, L.N. "N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation" . Redox Report 22, no. 6 (2017) : 265-271.
---------- MLA ----------
Soto, D., Gomez-Serrano, M., Pieralisi, A., Calvo, J.C., Peral, B., Guerra, L.N. "N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation" . Redox Report, vol. 22, no. 6, 2017, pp. 265-271.
---------- VANCOUVER ----------
Soto, D., Gomez-Serrano, M., Pieralisi, A., Calvo, J.C., Peral, B., Guerra, L.N. N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation. Redox Rep. 2017;22(6):265-271.