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Abstract:

Objectives: Reports investigating the effects of antioxidants on obesity have provided contradictory results. We have previously demonstrated that treatment with the antioxidant N-acetylcysteine (NAC) inhibits cellular triglyceride (Tg) accumulation as well as total cellular monoamine oxidase A (MAOA) expression in 3T3-L1 mature adipocytes (Calzadilla et al., Redox Rep. 2013;210–218). Here we analyzed the role of NAC on adipogenic differentiation pathway. Methods: Assays were conducted using 3T3-L1 preadipocytes (undifferentiated cells: CC), which are capable of differentiating into mature adipocytes (differentiated cells: DC). We studied the effects of different doses of NAC (0.01 or 1 mM) on DC, to evaluate cellular expression of phospho-JNK½ (pJNK½), phospho-ERK½ (pERK½) and, mitochondrial expression of citrate synthase, fumarate hydratase and MAOA. Results: Following the differentiation of preadipocytes, an increase in the expression levels of pJNK½ and pERK½ was observed, together with mitotic clonal expansion (MCE). We found that both doses of NAC decreased the expression of pJNK½ and pERK½. Consistent with these results, NAC significantly inhibited MCE and modified the expression of different mitochondrial proteins. Discussion: Our results suggested that NAC could inhibit Tg and mitochondrial protein expression by preventing both MCE and kinase phosphorylation. © 2016 Informa UK Limited, trading as Taylor & Francis Group.

Registro:

Documento: Artículo
Título:N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation
Autor:Soto, D.; Gomez-Serrano, M.; Pieralisi, A.; Calvo, J.C.; Peral, B.; Guerra, L.N.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires–IQUIBICEN, CONICET, Buenos Aires, Argentina
Instituto de Investigaciones Biomédicas Alberto Sols, Consejo Superior de Investigaciones Científicas (CSIC) y Universidad Autónoma de Madrid, (CSIC, UAM), Madrid, Spain
IBYME, CONICET, Buenos Aires, Argentina
Palabras clave:3T3-L1; adipocyte differentiation; Antioxidant; kinase phosphorylation; MAPK; mitocondria; monoamine oxidase A; obesity; acetylcysteine; amine oxidase (flavin containing) isoenzyme A; citrate synthase; fumarate hydratase; mitochondrial protein; mitogen activated protein kinase 1; mitogen activated protein kinase 3; mitogen activated protein kinase p38; stress activated protein kinase 1; acetylcysteine; amine oxidase (flavin containing); antioxidant; adipocyte; adipogenesis; Article; cell count; cell differentiation; citric acid cycle; controlled study; mitochondrion; mitosis; priority journal; proadipocyte; protein expression; protein phosphorylation; 3T3-L1 cell line; adipocyte; animal; cell differentiation; cytology; drug effect; metabolism; mouse; phosphorylation; 3T3-L1 Cells; Acetylcysteine; Adipocytes; Animals; Antioxidants; Cell Differentiation; Mice; Monoamine Oxidase; Phosphorylation
Año:2017
Volumen:22
Número:6
Página de inicio:265
Página de fin:271
DOI: http://dx.doi.org/10.1080/13510002.2016.1223267
Título revista:Redox Report
Título revista abreviado:Redox Rep.
ISSN:13510002
CODEN:RDRPE
CAS:acetylcysteine, 616-91-1; citrate synthase, 9027-96-7; fumarate hydratase, 9032-88-6; mitogen activated protein kinase 1, 137632-08-7; mitogen activated protein kinase 3, 137632-07-6; amine oxidase (flavin containing), 37255-42-8, 9001-66-5, 9059-11-4; Acetylcysteine; Antioxidants; Monoamine Oxidase
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13510002_v22_n6_p265_Soto

Referencias:

  • Gummersbach, C., Hemmrich, K., Klaus-Dietrich, K., Suschek, C.V., Fehsel, K., Pallua, N., New aspect of adipogenesis: radicals and oxidative stress (2009) Differentiation., 77, pp. 115-120
  • Lee, H., Jeong, Y., Choi, H., Ko, E., Kim, J., Reactive oxygen species facilitates adipocyte differentiation by accelerating mitotic clonal expansion (2009) J Biol Chem., 284, pp. 10601-10609
  • Calzadilla, P., Sapochnik, D., Cosentino, S., N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes (2011) IJMS., 12, pp. 6936-6951
  • Calzadilla, P., Gomez-Serrano, M., García-Santos, E., N-acetylcysteine affects obesity proteins expression in 3T3-L1 (2013) Redox Rep., 18, pp. 210-218
  • Bost, F., Aouadi, M., Caron, L., Binetruy, B., The role of MAPKs in adipocyte differentiation and obesity (2005) Biochimie., 87, pp. 51-56
  • Prusty, D., Park, B.H., Davis, K.E., Farmer, S.R., Activation of MEK/ERK signaling promotes adipogenesis by enhancing peroxisome proliferator-activated receptor gamma (PPARgamma) and C/EBP alpha gene expression during differentiation of 3T3-L1 preadipocytes (2002) J Biol Chem., 277, pp. 46226-46232
  • Donzelli, E., Lucchini, C., Ballarini, E., ERK1 and ERK 2 are involved in recruitment and maturation of human mesenchymal stem cells induced to adipogenic differentiation (2011) J Mol Cell Biol., 3, pp. 123-131
  • Droge, W., Free radicals in the physiological control of cell function (2002) Physiol Rev., 82, pp. 47-95
  • Cardaci, S., Ciriolo, M.R., TCA cycle defects and cancer: when metabolism tunes redox state (2012) Int J Cell Biol.
  • Lindinger, P.W., Christe, M., Eberle, A.N., Important mitochondrial proteins in human omental adipose tissue show reduced expression in obesity (2015) J Proteomics., 124, pp. 79-87
  • Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal Biochem., 72, pp. 248-254
  • (2003) Statistix 8, , Tallahassee, FL, USA: McGraw–Hill/Irwin
  • Yano, M., Hoogenradd, N., Terada, K., Takamori, M., Identification and functional analysis of human Tomm22 for protein import into mitocondria (2000) Mol Cell Biol., 20, pp. 7205-7213
  • Bost, F., Caron, L., Marchetti, I., Dani, C., Marchand-Brustel, Y., Binetry, B., Retinoic acid activation of the ERK pathway is required for embryonic stem cell commitment into the adipocytelineage (2002) Biochem J., 361, pp. 621-627
  • Anjum, R., Blenis, J., The RSK family of kinases: emerging roles in cellular signaling (2008) Nat Rev Mol Cell. Biol., 9, pp. 747-758
  • Xu, J., Liao, K., Protein kinase B/AKT 1 plays a pivotal role in insulin-like growth factor-1 receptor signaling induced 3T3-L1 adipocyte differentiation (2004) J Biol Chem., 279, pp. 35914-35922
  • Fukuoka, H., Iida, K., Nishizawa, H., IGF-1 stimulates reactive oxygen species (ROS) production and inhibits insulin-dependent glucose uptake via ROS in 3T3-L1 adipocytes (2010) Growth Horm IGF Res., 20, pp. 212-219
  • Mc Garry, D., Chakravarty, P., Wolf, C.R., Henderson, C.J., Altered protein S-glutathionylation identifies a potential mechanism of resistance to acetaminophen-induced hepatotoxicity (2015) J Pharmacol Exp Ther., 355, pp. 137-144
  • Adler, V., Yin, Z., Fuchs, S.Y., Regulation of JNK signaling by GSTp (1999) EMBO J., 18, pp. 1321-1334
  • MacDougald, O.A., Lane, M.D., Transcriptional regulation of gene expression during adipocyte differentiation (1995) Annu Rev Biochem., 64, pp. 345-358
  • Tang, Q.-Q., Otto, T.C., Lane, M.D., Mitotic clonal expansion: a synchronous process required for adipogenesis (2003) PNAS., 100, pp. 44-49
  • Tang, Q.-Q., Otto, T.C., Lane, M.D., CCAT/enhancer-binding protein beta is required for mitotic clonal expansion during adipogenesis (2003) PNAS., 100, pp. 850-855
  • Zhang, Y., Li, X., Qian, S.-W., Transcriptional activation of histone H4 by C/EBPβ during the mitotic clonal expansion of 3T3-L1 adipocyte differentiation (2011) Mol Biol Cell., 22, pp. 2165-2174
  • Chang, Y.C., Yu, Y.H., Shew, J.Y., Deficiency of NPGPx, an oxidative stress sensor, leads to obesity in mice and human (2013) EMBO Mol Med., 5, pp. 1165-1179
  • Cummins, T.D., Holden, C.R., Sansbury, B.E., Metabolic remodeling of white adipose tissue in obesity (2014) Am J Physiol Endocrinol Metab., 307, pp. 262-277
  • Ashrafian, H., O’Flaherty, L., Adam, J., Expression profiling in progressive stages of fumarate-hydratase deficiency: the contribution of metabolic changes to tumorigenesis (2010) Cancer Res., 70, pp. 9153-9165
  • Bianchi, P., Kunduzova, O., Masini, E., Oxidative stress by monoamine oxidase mediates receptor-independent cardiomyocyte apoptosis by serotonin and postischemic myocardial injury (2005) Circulation., 112, pp. 3297-3305
  • Ou, X.M., Chen, K., Shih, J.C., Monoamine oxidase A and repressor R1 are involved in apoptotic signaling pathway (2006) PNAS., 103, pp. 10923-10928
  • Alleman, R.J., Katunga, L.A., Nelson, M.A., Brown, D.A., Anderson, E.J., The Goldilocks Zone from a redox perspective–adaptive vs. deleterious responses to oxidative stress in striated muscle (2014) Front Physiol., 18, p. 358
  • Kunduzova, O.R., Bianchi, P., Pizzinat, N., Regulation of JNK/ERK activation, cell apoptosis and tissue regeneration by monoamine oxidases after renal ischemia-reperfusion (2002) FASEB J., 16, pp. 1129-1131
  • Pastore, A., Piemonte, F., Locatelli, M., Determination of blood total, reduced, and oxidized glutathione in pediatric subjects (2001) Clin Chem., 47, pp. 1467-1469
  • De Tursi Rispoli, L., Vazquez Tarragon, A., Vazquez Prado, A., Saez Tormo, G., Ismail, A., Gumbau Puchol, V., Oxidative stress: a comparative study between normal and morbid obesity group population (2013) Nutr Hosp., 28, pp. 671-675
  • Hildebrandt, W., Hamann, A., Krakowski-Roosen, H., Effect of thiol antioxidant on body fat and insulin reactivity (2004) J Mol Med., 82, pp. 336-344
  • Bahmani, F., Karamali, M., Shareki, H., Asemi, Z., The effects of folate supplementation on inflammatory factors and biomarkers of oxidative stress in overweight and obese women with polycystic ovary síndrome: a randomized, double blind, placebo controlled clinical trial (2014) Clin Endocrinol., 81, pp. 582-587

Citas:

---------- APA ----------
Soto, D., Gomez-Serrano, M., Pieralisi, A., Calvo, J.C., Peral, B. & Guerra, L.N. (2017) . N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation. Redox Report, 22(6), 265-271.
http://dx.doi.org/10.1080/13510002.2016.1223267
---------- CHICAGO ----------
Soto, D., Gomez-Serrano, M., Pieralisi, A., Calvo, J.C., Peral, B., Guerra, L.N. "N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation" . Redox Report 22, no. 6 (2017) : 265-271.
http://dx.doi.org/10.1080/13510002.2016.1223267
---------- MLA ----------
Soto, D., Gomez-Serrano, M., Pieralisi, A., Calvo, J.C., Peral, B., Guerra, L.N. "N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation" . Redox Report, vol. 22, no. 6, 2017, pp. 265-271.
http://dx.doi.org/10.1080/13510002.2016.1223267
---------- VANCOUVER ----------
Soto, D., Gomez-Serrano, M., Pieralisi, A., Calvo, J.C., Peral, B., Guerra, L.N. N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation. Redox Rep. 2017;22(6):265-271.
http://dx.doi.org/10.1080/13510002.2016.1223267