Abstract:
Objectives: This study was focused on the role of indole acetic acid (IAA) in the defense against oxidative stress damage caused by drought in soybean plants and to elucidate whether heme oxygenase-1 (HO-1) and nitric oxide (NO) are involved in this mechanism. IAA is an auxin that participates in many plant processes including oxidative stress defense, but to the best of our knowledge no information is yet available about its possible action in drought stress. Methods: To this end, soybean plants were treated with 8% polyethylene glycol (PEG) or 100 μM IAA. To evaluate the behavior of IAA, plants were pretreated with this compound previous to PEG addition. Lipid peroxidation levels (thiobarbituric acid reactive substances (TBARS)), glutathione (GSH) and ascorbate (AS) contents, catalase (CAT), superoxide dismutase (SOD), and guaiacol peroxidase (POD) activities were determined to evaluate oxidative damage. Results: Drought treatment (8% PEG) caused a significant increase in TBARS levels as well as a marked decrease in the non-enzymatic (GSH and AS) and enzymatic (CAT, SOD, and POD) antioxidant defense systems. Pre-treatment with IAA prevented the alterations of stress parameters caused by drought, while treatment with IAA alone did not produce changes in TBARS levels, or GSH and AS contents. Moreover, the activities of the classical enzymes involved in the enzymatic defense system (SOD, CAT, and POD) remained similar to control values. Furthermore, this hormone could enhance HO-1 activity (75% with respect to controls), and this increase was positively correlated with protein content as well as gene expression. The direct participation of HO-1 as an antioxidant enzyme was established by performing experiments in the presence of Zn-protoporphyrin IX, a well-known irreversible inhibitor of this enzyme. It was also demonstrated that HO-1 is modulated by NO, as shown by experiments performed in the presence of an NO donor (sodium nitroprusside), an NO scavenger (2-(4-carboxyphenyl)-4,4,5,5- tetramethylimidazoline-1-oxyl-3-oxide), or an NO synthesis inhibitor (N-nitro-L-arginine methyl ester, NAME). Discussion: It is concluded that IAA is responsible, at least in part, for the protection against oxidative stress caused by drought in soybean plants through the modulation of NO levels which, in turn, enhances HO-1 synthesis and activity. © W. S. Maney & Son Ltd 2014.
Registro:
Documento: |
Artículo
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Título: | Indole acetic acid is responsible for protection against oxidative stress caused by drought in soybean plants: The role of heme oxygenase induction |
Autor: | Lecube, M.L.; Noriega, G.O.; Santa Cruz, D.M.; Tomaro, M.L.; Batlle, A.; Balestrasse, K.B. |
Filiación: | Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), CONICET, Universidad de Buenos Aires, Argentina Departamento de Química Biológica, Universidad de Buenos Aires, Argentina Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA), CONICET, Buenos Aires, Argentina Departamento de Biología Aplicada y Alimentos, Universidad de Buenos Aires, Argentina
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Palabras clave: | Drought stress; Glycine max. L; Heme oxygenase; Indole acetic acid; Nitric oxide; ascorbic acid; catalase; glutathione; guaiacol peroxidase; heme oxygenase 1; indoleacetic acid; macrogol; nitric oxide; nitroprusside sodium; protoporphyrin zinc; superoxide dismutase; thiobarbituric acid reactive substance; zinc ion; antioxidant; heme oxygenase; indoleacetic acid; indoleacetic acid derivative; macrogol derivative; Article; drought stress; enzyme activity; gene expression; lipid peroxidation; nonhuman; oxidative stress; plant parameters; protein content; soybean; chemistry; drought; enzymology; metabolism; physiological stress; soybean; Glycine max; Antioxidants; Ascorbic Acid; Droughts; Glutathione; Heme Oxygenase (Decyclizing); Indoleacetic Acids; Lipid Peroxidation; Nitric Oxide; Oxidative Stress; Polyethylene Glycols; Soybeans; Stress, Physiological; Thiobarbituric Acid Reactive Substances |
Año: | 2014
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Volumen: | 19
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Número: | 6
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Página de inicio: | 242
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Página de fin: | 250
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DOI: |
http://dx.doi.org/10.1179/1351000214Y.0000000095 |
Título revista: | Redox Report
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Título revista abreviado: | Redox Rep.
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ISSN: | 13510002
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CODEN: | RDRPE
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CAS: | ascorbic acid, 134-03-2, 15421-15-5, 50-81-7; catalase, 9001-05-2; glutathione, 70-18-8; indoleacetic acid, 32536-43-9, 87-51-4; macrogol, 25322-68-3; nitric oxide, 10102-43-9; nitroprusside sodium, 14402-89-2, 15078-28-1; protoporphyrin zinc, 15442-64-5; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; zinc ion, 23713-49-7; heme oxygenase, 9059-22-7; Antioxidants; Ascorbic Acid; Glutathione; Heme Oxygenase (Decyclizing); indoleacetic acid; Indoleacetic Acids; Nitric Oxide; Polyethylene Glycols; Thiobarbituric Acid Reactive Substances
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13510002_v19_n6_p242_Lecube |
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Citas:
---------- APA ----------
Lecube, M.L., Noriega, G.O., Santa Cruz, D.M., Tomaro, M.L., Batlle, A. & Balestrasse, K.B.
(2014)
. Indole acetic acid is responsible for protection against oxidative stress caused by drought in soybean plants: The role of heme oxygenase induction. Redox Report, 19(6), 242-250.
http://dx.doi.org/10.1179/1351000214Y.0000000095---------- CHICAGO ----------
Lecube, M.L., Noriega, G.O., Santa Cruz, D.M., Tomaro, M.L., Batlle, A., Balestrasse, K.B.
"Indole acetic acid is responsible for protection against oxidative stress caused by drought in soybean plants: The role of heme oxygenase induction"
. Redox Report 19, no. 6
(2014) : 242-250.
http://dx.doi.org/10.1179/1351000214Y.0000000095---------- MLA ----------
Lecube, M.L., Noriega, G.O., Santa Cruz, D.M., Tomaro, M.L., Batlle, A., Balestrasse, K.B.
"Indole acetic acid is responsible for protection against oxidative stress caused by drought in soybean plants: The role of heme oxygenase induction"
. Redox Report, vol. 19, no. 6, 2014, pp. 242-250.
http://dx.doi.org/10.1179/1351000214Y.0000000095---------- VANCOUVER ----------
Lecube, M.L., Noriega, G.O., Santa Cruz, D.M., Tomaro, M.L., Batlle, A., Balestrasse, K.B. Indole acetic acid is responsible for protection against oxidative stress caused by drought in soybean plants: The role of heme oxygenase induction. Redox Rep. 2014;19(6):242-250.
http://dx.doi.org/10.1179/1351000214Y.0000000095