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Abstract:

Objectives: This study was focused on the role of indole acetic acid (IAA) in the defense against oxidative stress damage caused by drought in soybean plants and to elucidate whether heme oxygenase-1 (HO-1) and nitric oxide (NO) are involved in this mechanism. IAA is an auxin that participates in many plant processes including oxidative stress defense, but to the best of our knowledge no information is yet available about its possible action in drought stress. Methods: To this end, soybean plants were treated with 8% polyethylene glycol (PEG) or 100 μM IAA. To evaluate the behavior of IAA, plants were pretreated with this compound previous to PEG addition. Lipid peroxidation levels (thiobarbituric acid reactive substances (TBARS)), glutathione (GSH) and ascorbate (AS) contents, catalase (CAT), superoxide dismutase (SOD), and guaiacol peroxidase (POD) activities were determined to evaluate oxidative damage. Results: Drought treatment (8% PEG) caused a significant increase in TBARS levels as well as a marked decrease in the non-enzymatic (GSH and AS) and enzymatic (CAT, SOD, and POD) antioxidant defense systems. Pre-treatment with IAA prevented the alterations of stress parameters caused by drought, while treatment with IAA alone did not produce changes in TBARS levels, or GSH and AS contents. Moreover, the activities of the classical enzymes involved in the enzymatic defense system (SOD, CAT, and POD) remained similar to control values. Furthermore, this hormone could enhance HO-1 activity (75% with respect to controls), and this increase was positively correlated with protein content as well as gene expression. The direct participation of HO-1 as an antioxidant enzyme was established by performing experiments in the presence of Zn-protoporphyrin IX, a well-known irreversible inhibitor of this enzyme. It was also demonstrated that HO-1 is modulated by NO, as shown by experiments performed in the presence of an NO donor (sodium nitroprusside), an NO scavenger (2-(4-carboxyphenyl)-4,4,5,5- tetramethylimidazoline-1-oxyl-3-oxide), or an NO synthesis inhibitor (N-nitro-L-arginine methyl ester, NAME). Discussion: It is concluded that IAA is responsible, at least in part, for the protection against oxidative stress caused by drought in soybean plants through the modulation of NO levels which, in turn, enhances HO-1 synthesis and activity. © W. S. Maney & Son Ltd 2014.

Registro:

Documento: Artículo
Título:Indole acetic acid is responsible for protection against oxidative stress caused by drought in soybean plants: The role of heme oxygenase induction
Autor:Lecube, M.L.; Noriega, G.O.; Santa Cruz, D.M.; Tomaro, M.L.; Batlle, A.; Balestrasse, K.B.
Filiación:Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), CONICET, Universidad de Buenos Aires, Argentina
Departamento de Química Biológica, Universidad de Buenos Aires, Argentina
Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA), CONICET, Buenos Aires, Argentina
Departamento de Biología Aplicada y Alimentos, Universidad de Buenos Aires, Argentina
Palabras clave:Drought stress; Glycine max. L; Heme oxygenase; Indole acetic acid; Nitric oxide; ascorbic acid; catalase; glutathione; guaiacol peroxidase; heme oxygenase 1; indoleacetic acid; macrogol; nitric oxide; nitroprusside sodium; protoporphyrin zinc; superoxide dismutase; thiobarbituric acid reactive substance; zinc ion; antioxidant; heme oxygenase; indoleacetic acid; indoleacetic acid derivative; macrogol derivative; Article; drought stress; enzyme activity; gene expression; lipid peroxidation; nonhuman; oxidative stress; plant parameters; protein content; soybean; chemistry; drought; enzymology; metabolism; physiological stress; soybean; Glycine max; Antioxidants; Ascorbic Acid; Droughts; Glutathione; Heme Oxygenase (Decyclizing); Indoleacetic Acids; Lipid Peroxidation; Nitric Oxide; Oxidative Stress; Polyethylene Glycols; Soybeans; Stress, Physiological; Thiobarbituric Acid Reactive Substances
Año:2014
Volumen:19
Número:6
Página de inicio:242
Página de fin:250
DOI: http://dx.doi.org/10.1179/1351000214Y.0000000095
Título revista:Redox Report
Título revista abreviado:Redox Rep.
ISSN:13510002
CODEN:RDRPE
CAS:ascorbic acid, 134-03-2, 15421-15-5, 50-81-7; catalase, 9001-05-2; glutathione, 70-18-8; indoleacetic acid, 32536-43-9, 87-51-4; macrogol, 25322-68-3; nitric oxide, 10102-43-9; nitroprusside sodium, 14402-89-2, 15078-28-1; protoporphyrin zinc, 15442-64-5; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; zinc ion, 23713-49-7; heme oxygenase, 9059-22-7; Antioxidants; Ascorbic Acid; Glutathione; Heme Oxygenase (Decyclizing); indoleacetic acid; Indoleacetic Acids; Nitric Oxide; Polyethylene Glycols; Thiobarbituric Acid Reactive Substances
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13510002_v19_n6_p242_Lecube

Referencias:

  • Bajaj, S., Targolli, J., Liu, L., Ho, T., Wu, R., Transgenic approaches to increase dehydration-stress tolerance in plants (1999) Mol Breed, 5, pp. 493-503
  • Smirnoff, N., Plant resistance to environmental stress (1998) Curr Opin Biotech, 9 (2), pp. 214-219
  • Beligni, M., Lamattina, L., Nitric oxide counteracts cytotoxic processes mediated by reactive oxygen species in plant tissues (1999) Planta, 208 (3), pp. 337-344
  • Asada, K., Mechanisms for scavenging reactive molecules generated in chloroplasts under light stress (1994) From molecular mechanisms to the field, pp. 129-142. , Baker NR, Bowyer JR, (eds.) Photoinhibition of photosynthesis, Oxford: Bios Scientific Publishers
  • Pustovoitova, T., Zholkevich, V., Basic trends in the investigation of drought effects on physiological processes in plants (1992) Fiziol Biokhim Kul't Rast, 24, pp. 14-27
  • Zholkevich, V., Pustovoitova, T., Growth and phytohormone content in Cucumis sativus L. leaves under water deficiency (1993) Russ Plant Physiol, 40, pp. 595-599
  • Zhang, X., Ervin, E.H., Evanylo, G.K., Haering, K.C., Impact of biosolids on hormone metabolism in drought-stressed tall fescue (2009) Crop Sci, 49 (5), pp. 1893-1901
  • Muramoto, T., Tsurui, N., Terry, M., Yokota, A., Kohchi, T., Expression and biochemical properties of a ferredoxin-dependent heme oxygenase required for phytochrome chromophore synthesis (2002) Plant Physiol, 130, pp. 1958-1966
  • Terry, M., Linley, P., Kohchi, O., Making light of it: The role of plant haem oxygenases in phytochrome chromophore synthesis (2002) Biochem Soc Trans, 30, pp. 604-609
  • Gohya, T., Zhang, X., Yoshida, T., Migita, C., Spectroscopic characterization of a higher plant heme oxygenase isoform-1 from Glycine max (soybean): Coordination structure of the heme complex and catabolism of heme (2006) FEBS J, 273, pp. 5384-5399
  • Synder, X., Baranano, D., Heme oxygenase: A font of multiple messengers (2001) Neuropsychopharmacology, 25, pp. 294-298
  • Davis, S., Kurepa, J., Vierstra, R., The Arabidopsis thaliana HY1 locus, required for phytochrome-chromophore biosynthesis, encodes a protein related to heme oxygenases (1999) Proc Natl Acad Sci USA, 96, pp. 6541-6546
  • Davis, S., Bhoo, S., Durski, A., Walter, J., Vierstra, R., The heme-oxygenase family required for phytochrome chromophore biosynthesis is necessary for proper photomorphogenesis in higher plants (2001) Plant Physiol, 126, pp. 656-659
  • Emborg, T., Walker, J., Noh, B., Vierstra, R., Multiple heme oxygenase family members contribute to the biosynthesis of the phytochrome chromophore in Arabidopsis (2006) Plant Physiol, 140, pp. 856-968
  • Gisk, B., Yasui, Y., Kohchi, T., Frankenbeg-Dinkel, N., Characterization of the haem oxygenase protein family in Arabidopsis (2010) Biochem J, 425 (2), pp. 425-434
  • Noriega, G., Balestrasse, K., Batlle, A., Tomaro, M., Heme oxygenase exerts a protective role against oxidative stress in soybean leaves (2004) Biochem Biophys Res Commun, 323, pp. 1003-1008
  • Shen, Q., Jiang, M., Li, H., Che, L., Yang, Z., Expression of a Brasssica napus heme oxygenase confers plant tolerance to mercury toxicity (2011) Plant Cell Environ, 34, pp. 752-763
  • Xuan, W., Zhu, F., Xu, S., Huang, B., Ling, T., Qi, J., The heme oxygenase/carbon monoxide system is involved in the auxininduced cucumber adventitious root process (2008) Plant Physiol, 148, pp. 881-893
  • Guo, K., Kong, W., Yang, Z., Carbon monoxide promotes root hair development in tomato (2009) Plant Cell Environ, 32, pp. 1033-1045
  • Noriega, G., Yannarelli, G., Balestrasse, K., Batlle, A., Tomaro, M., The effect of nitric oxide on heme oxygenase gene expression in soybean leaves (2007) Planta, 226, pp. 1155-1163
  • Stocker, R., Induction of haemoxygenase as a defence against oxidative stress (1990) Free Radic Res Commun, 9, pp. 101-112
  • Dixit, S., Verma, K., Shekhawat, G., In vitro evaluation of mitochondrial-chloroplast subcellular localization of heme oxygenase1 (HO1) in Glycine max (2014) Protoplasma, 251 (3), pp. 671-675
  • Zilli, C., Santa-Cruz, D., Polizio, A., Tomaro, M., Balestrasse, K., Symbiotic association between soybean plants and Bradyrhizobium japonicum develops oxidative stress and heme oxygenase-1 induction at early stages (2011) Redox Rep, 16 (2), pp. 49-55
  • Saxena, I., Shekhawat, G., Nitric oxide (NO) in alleviation of heavy metal induced phytotoxicity and its role in protein nitration (2013) Nitric Oxide, 32, pp. 13-20
  • Santa-Cruz, D., Pacienza, N., Polizio, A., Balestrasse, K., Tomaro, M., Yannarelli, G., Nitric oxide synthase-like dependent NO production enhances heme oxygenase up-regulation in ultraviolet-B-irradiated soybean plants (2010) Phytochemistry, 71 (14-15), pp. 1000-1007
  • Huang, J., Han, B., Xu, S., Zhou, M., Shen, W., Heme oxygenase-1 is involved in the cytokinin-induced alleviation of senescence in detached wheat leaves during dark incubation (2011) J Plant Physiol, 168, pp. 768-775
  • Wei, Y., Zheng, Q., Liu, Z., Yang, Z., Regulation of tolerance of Chlamydomonas reinhardtii to heavy metal toxicity by heme oxgenase-1 and carbon monoxide (2011) Plant Cell Physiol, 52, pp. 1665-1675
  • Xu, S., Zhang, B., Cao, Z., Ling, T., Shen, W., Heme oxygenase is involved in cobalt chloride-induced lateral root development in tomato (2011) Biometals, 24 (2), pp. 181-191
  • Otterbein, L., Bach, F., Alam, J., Soares, M., Tao, H., Wysk, M., Carbon monoxide mediates anti-inflammatory effects via the mitogen activated protein kinase pathway (2000) Nat Med, 6, p. 422
  • Xuan, W., Zhu, F., Xu, S., Huang, B., Ling, T., Qi, J., The heme oxygenase/carbon monoxide system is involved in the auxininduced cucumber adventitious rooting process (2008) Plant Physiol, 148 (2), pp. 881-893
  • Shekhawat, G., Verma, K., Haem oxygenase (HO): An overlooked enzyme of plant metabolism and defence (2010) J Exp Bot, 61, pp. 2255-2270
  • Fang, T., Li, J., Cao, Z., Chen, M., Shen, W., Huang, L., Heme oxygenase-1 is involved in sodium hydrosulfide-induced lateral root formation in tomato seedlings (2014) Plant Cell Rep, 33, pp. 969-978
  • He, H., He, L., Heme-oxygenase-1 and abiotic stresses in plants (2014) Acta Physiol Plant, 36 (3), pp. 581-588
  • Romero-Puertas, M., Perazzolli, M., Zago, E., Delledonne, M., Nitric oxide signaling functions in plant-pathogen interactions (2004) Cell Microbiol, 6 (9), pp. 795-808
  • Corpas, F., Palma, J., Del Río, L., Barroso, J., Evidence supporting the existence of L-arginine-dependent nitric oxide synthase activity in plants (2009) New Phytol, 184, pp. 9-14
  • Leitner, M., Vandelle, E., Gaupels, F., Bellin, D., Delledonne, M., NO signals in the haze: Nitric oxide signalling in plant defence (2009) Curr Opin Plant Biol, 12 (4), pp. 451-464
  • Parani, M., Rudrabhatla, S., Myers, R., Weirich, H., Smith, B., Leaman, D., Microarray analysis of nitric oxide responsive transcripts in Arabidopsis (2004) Plant Biotechnol J, 2 (4), pp. 359-368
  • Mackerness, S., John, C., Jordan, B., Thomas, B., Early signaling components in ultraviolet-B responses: Distinct roles for different reactive oxygen species and nitric oxide (2001) FEBS Lett, 489, pp. 237-242
  • Chen, Y., Kao, C., Calcium is involved in nitric oxide-and auxininduced lateral root formation in rice (2012) Protoplasma, 249, pp. 187-195
  • Lamattina, L., García-Mata, C., Graziano, M., Pagnussat, G., Nitric oxide: The versatility of an extensive signal molecule (2003) Annu Rev Plant Biol, 54, pp. 109-136
  • Shantel, A., Fowler, R., Virgen, A., Gossett, D., Banks, S., Rodriguez, S., Opposing roles for superoxide and nitric oxide in the NaCl stress-induced upregulation of antioxidant enzyme activity in cotton callus tissue (2008) Environ Exp Bot, 62, pp. 60-68
  • Heath, R., Packer, L., Photoperoxidation in isolated chloroplasts. Kinetics and stoichiometry of fatty acid peroxidation (1968) Arch Biochem Biophys, 125, pp. 189-198
  • Law, M., Charles, S., Halliwell, B., Glutathione and ascorbic acid in spinach (Spinacia oleracea) chloroplasts. The effect of hydrogen peroxide and of paraquat (1983) Biochem J, 210, pp. 899-903
  • Chance, B., Sies, H., Boveris, A., Hydroperoxide metabolism in mammalian organs (1979) Physiol Rev, 72, pp. 527-605
  • Becana, M., Aparicio-Tejo, P., Irigoyen, J., Sánchez-Díaz, M., Some enzymes of hydrogen peroxide metabolism in leaves and root nodules of Medicago sativa (1986) Plant Physiol, 82, pp. 1169-1171
  • Muramoto, T., Kohchi, T., Yokota, A., Hwang, I., Goodman, H.M., The Arabidopsis photomorphogenic mutant HY1 is deficient in phytochrome chromophore biosynthesis as a result of a mutation in a plastid haeme oxygenase (1999) Plant Cell, 11, pp. 335-348
  • Laemmli, U., Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685
  • Yannarelli, G., Noriega, G., Batlle, A., Tomaro, M., Heme oxygenase up-regulation in ultraviolet-B irradiated soybean plants involves reactive oxygen species (2006) Planta, 224, pp. 1154-1162
  • Bradford, M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal Biochem, 72, pp. 248-254
  • Pagnussat, G., Simontacchi, M., Puntarulo, S., Lamattina, L., Nitric oxide is required for root organogenesis (2002) Plant Physiol, 129, pp. 954-956
  • Pagnussat, G., Lanteri, M., Lamattina, L., Nitric oxide and cyclic GMP are messengers in the IAA-induced adventitious rooting process (2003) Plant Physiol, 132, pp. 1241-1248
  • Finnie, J., Van Staden, J., Effect of seed weeds concentrate and applied hormones on in vitro cultured tomato roots (1985) J Plant Physiol, 120, pp. 215-222
  • Cruz de Carvalho, M., Drought stress and reactive oxygen species (2008) Plant Signal Behav, 3, pp. 156-165
  • Siddiqui, M., Al-Whaibi, M., Basalah, M., Role of nitric oxide in tolerance of plants to abiotic stress (2011) Protoplasma, 248 (3), pp. 447-455
  • Hao, Z., Li, X., Su, Z., Xie, C., Li, M., Liang, X., A proposed selection criterion for drought resistance across multiple environments in maize (2011) Breed Sci, 61, pp. 101-108
  • Zhi-Sheng, S., Li-Qin, H., Guo-Lin, W., Jin-Peng, D., Xiao-Yue, C., Tian, Y., Carbon monoxide: A novel antioxidant against oxidative stress in wheat seedling leaves (2007) J Integr Plant Biol, 49, pp. 638-645
  • Bai, X.G., Chen, J.H., Kong, X.X., Todd, C.D., Yang, Y.P., Hu, X.Y., Carbon monoxide enhances the chilling tolerance of recalcitrant Baccaurea ramiflora seeds via nitric oxide-mediated glutathione homeostasis (2012) Free Rad Biol Med, 53, pp. 710-720

Citas:

---------- APA ----------
Lecube, M.L., Noriega, G.O., Santa Cruz, D.M., Tomaro, M.L., Batlle, A. & Balestrasse, K.B. (2014) . Indole acetic acid is responsible for protection against oxidative stress caused by drought in soybean plants: The role of heme oxygenase induction. Redox Report, 19(6), 242-250.
http://dx.doi.org/10.1179/1351000214Y.0000000095
---------- CHICAGO ----------
Lecube, M.L., Noriega, G.O., Santa Cruz, D.M., Tomaro, M.L., Batlle, A., Balestrasse, K.B. "Indole acetic acid is responsible for protection against oxidative stress caused by drought in soybean plants: The role of heme oxygenase induction" . Redox Report 19, no. 6 (2014) : 242-250.
http://dx.doi.org/10.1179/1351000214Y.0000000095
---------- MLA ----------
Lecube, M.L., Noriega, G.O., Santa Cruz, D.M., Tomaro, M.L., Batlle, A., Balestrasse, K.B. "Indole acetic acid is responsible for protection against oxidative stress caused by drought in soybean plants: The role of heme oxygenase induction" . Redox Report, vol. 19, no. 6, 2014, pp. 242-250.
http://dx.doi.org/10.1179/1351000214Y.0000000095
---------- VANCOUVER ----------
Lecube, M.L., Noriega, G.O., Santa Cruz, D.M., Tomaro, M.L., Batlle, A., Balestrasse, K.B. Indole acetic acid is responsible for protection against oxidative stress caused by drought in soybean plants: The role of heme oxygenase induction. Redox Rep. 2014;19(6):242-250.
http://dx.doi.org/10.1179/1351000214Y.0000000095