Artículo

Calzadilla, P.; Gómez-Serrano, M.; García-Santos, E.; Schiappacasse, A.; Abalde, Y.; Calvo, J.C.; Peral, B.; Guerra, L.N. "N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes" (2013) Redox Report. 18(6):210-218
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Abstract:

Objectives: Oxidative stress plays critical roles in the pathogeneses of diabetes, hypertension, and atherosclerosis, but its effect on fat accumulation is still unclear. In this study, we analyzed the role of the well-known antioxidant and a glutathione (GSH) precursor N-acetylcysteine (NAC) in fat accumulation and the expression of obesity-associated proteins. Methods: We studied the effects of 10 μM NAC on obesity-related protein expression in cultured 3T3-L1 preadipocytes, which are able to differentiate into mature adipocytes and accumulate lipids. Results: NAC treatment inhibited fat accumulation and reduced the expression of obesity-related proteins, including monoamine oxidase A, heat shock protein 70 (HSP70), aminoacylase -1 (ACY-1), and transketolase. Discussion: Our results suggest that the effects of NAC on triglycerides (Tgs) and protein expression are correlated. In support of this, we showed that NAC treatment affected both the Tg synthesis pathway and the expression levels of proteins implicated in human obesity. © W. S. Maney & Son Ltd 2013.

Registro:

Documento: Artículo
Título:N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes
Autor:Calzadilla, P.; Gómez-Serrano, M.; García-Santos, E.; Schiappacasse, A.; Abalde, Y.; Calvo, J.C.; Peral, B.; Guerra, L.N.
Filiación:Departamento de Química Biológica, Universidad de Buenos Aires - IQUIBICEN, CONICET, Intendente Güiraldes 2160, Pabellón 2, (1428), Buenos Aires, Argentina
Instituto de Investigaciones Biomédicas, Alberto Sols, Consejo Superior de Investigaciones Científicas y Universidad Autónoma de Madrid, (CSIC UAM), E-28029 Madrid, Spain
IBYME, CONICET, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Adipose differentiation; N-acetylcysteine; Obesity proteins; acetylcysteine; amine oxidase (flavin containing) isoenzyme A; aminoacylase; fatty acid binding protein 4; heat shock protein 70; transketolase; triacylglycerol; adipogenesis; amino acid metabolism; animal cell; article; carbohydrate metabolism; cell differentiation; cell maturation; cell proliferation; cell strain 3T3; cell viability; concentration response; controlled study; drug effect; drug mechanism; lipid storage; lipogenesis; mouse; nonhuman; obesity; oxidative stress; priority journal; proadipocyte; protein expression; 3T3-L1 Cells; Acetylcysteine; Adipocytes; Adipogenesis; Amidohydrolases; Animals; Cell Differentiation; Fatty Acid-Binding Proteins; HSP72 Heat-Shock Proteins; Mice; Monoamine Oxidase; Oxidative Stress; Transketolase; Triglycerides
Año:2013
Volumen:18
Número:6
Página de inicio:210
Página de fin:218
DOI: http://dx.doi.org/10.1179/1351000213Y.0000000066
Título revista:Redox Report
Título revista abreviado:Redox Rep.
ISSN:13510002
CODEN:RDRPE
CAS:acetylcysteine, 616-91-1; aminoacylase, 9012-37-7; transketolase, 9014-48-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13510002_v18_n6_p210_Calzadilla

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Citas:

---------- APA ----------
Calzadilla, P., Gómez-Serrano, M., García-Santos, E., Schiappacasse, A., Abalde, Y., Calvo, J.C., Peral, B.,..., Guerra, L.N. (2013) . N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes. Redox Report, 18(6), 210-218.
http://dx.doi.org/10.1179/1351000213Y.0000000066
---------- CHICAGO ----------
Calzadilla, P., Gómez-Serrano, M., García-Santos, E., Schiappacasse, A., Abalde, Y., Calvo, J.C., et al. "N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes" . Redox Report 18, no. 6 (2013) : 210-218.
http://dx.doi.org/10.1179/1351000213Y.0000000066
---------- MLA ----------
Calzadilla, P., Gómez-Serrano, M., García-Santos, E., Schiappacasse, A., Abalde, Y., Calvo, J.C., et al. "N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes" . Redox Report, vol. 18, no. 6, 2013, pp. 210-218.
http://dx.doi.org/10.1179/1351000213Y.0000000066
---------- VANCOUVER ----------
Calzadilla, P., Gómez-Serrano, M., García-Santos, E., Schiappacasse, A., Abalde, Y., Calvo, J.C., et al. N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes. Redox Rep. 2013;18(6):210-218.
http://dx.doi.org/10.1179/1351000213Y.0000000066