Artículo

Miler, E.A.; Ríos de Molina, M. del C.; Domínguez, G.; Guerra, L.N. "Thyroid hormone effect in human hepatocytes" (2008) Redox Report. 13(4):185-191
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Abstract:

We have already demonstrated that a combined treatment of methimazole and an antioxidant mixture improved the condition of hyperthyroid patients both biochemically and clinically. Elevated thyroid hormone levels might trigger signs and symptoms of hyperthyroidism through the increase of free radicals. To study the direct effect of thyroid hormone on cellular markers of oxidative stress, we carried out in vitro assays in which 0.1-20.0 nM T3 (6.5-1300.0 ng/dl) doses were added to culture media of the human hepatocyte cell line Hep G2 for 1-24 h. T3 increased malondialdehyde (MDA) and intracellular oxidized glutathione (GSSG) levels; SOD activity was also higher with hormone treatment, whereas catalase and glutathione peroxidase activities showed no variation at different T3 doses and during all experimental times. When ascorbic acid was added to the culture, the MDA level decreased and SOD activity was increased. With higher doses of T3 (e.g. 200 nM), cell death occurred (69% of apoptotic cells). The increase in SOD activity was not enough to overcome the effect of T3 since MDA and GSSG remained high during a 24-h experiment. We showed a beneficial effect of ascorbic acid when cells were exposed to a T3 dose of 20 nM, a higher level of hormone than that achieved in hyperthyroidism. © 2008 W. S. Maney and Son Ltd.

Registro:

Documento: Artículo
Título:Thyroid hormone effect in human hepatocytes
Autor:Miler, E.A.; Ríos de Molina, M. del C.; Domínguez, G.; Guerra, L.N.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, 1428 Buenos Aires, Argentina
Palabras clave:Human hepatocytes; Oxidative stress; Thyroxine; ascorbic acid; catalase; free radical; glutathione disulfide; glutathione peroxidase; liothyronine; malonaldehyde; superoxide dismutase; antioxidant activity; apoptosis; article; cell culture; cell level; cell strain HepG2; controlled study; cytotoxicity; enzyme activity; human; human cell; in vitro study; liver cell; oxidative stress; priority journal; Antioxidants; Ascorbic Acid; Catalase; Cell Line, Tumor; Dose-Response Relationship, Drug; Glutathione Disulfide; Glutathione Peroxidase; Hepatocytes; Humans; Malondialdehyde; Superoxide Dismutase; Triiodothyronine
Año:2008
Volumen:13
Número:4
Página de inicio:185
Página de fin:191
DOI: http://dx.doi.org/10.1179/135100008X308920
Título revista:Redox Report
Título revista abreviado:Redox Rep.
ISSN:13510002
CODEN:RDRPE
CAS:ascorbic acid, 134-03-2, 15421-15-5, 50-81-7; catalase, 9001-05-2; glutathione disulfide, 27025-41-8; glutathione peroxidase, 9013-66-5; liothyronine, 6138-47-2, 6893-02-3; malonaldehyde, 542-78-9; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; Antioxidants; Ascorbic Acid, 50-81-7; Catalase, EC 1.11.1.6; Glutathione Disulfide, 27025-41-8; Glutathione Peroxidase, EC 1.11.1.9; Malondialdehyde, 542-78-9; Superoxide Dismutase, EC 1.15.1.1; Triiodothyronine, 6893-02-3
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13510002_v13_n4_p185_Miler

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Citas:

---------- APA ----------
Miler, E.A., Ríos de Molina, M. del C., Domínguez, G. & Guerra, L.N. (2008) . Thyroid hormone effect in human hepatocytes. Redox Report, 13(4), 185-191.
http://dx.doi.org/10.1179/135100008X308920
---------- CHICAGO ----------
Miler, E.A., Ríos de Molina, M. del C., Domínguez, G., Guerra, L.N. "Thyroid hormone effect in human hepatocytes" . Redox Report 13, no. 4 (2008) : 185-191.
http://dx.doi.org/10.1179/135100008X308920
---------- MLA ----------
Miler, E.A., Ríos de Molina, M. del C., Domínguez, G., Guerra, L.N. "Thyroid hormone effect in human hepatocytes" . Redox Report, vol. 13, no. 4, 2008, pp. 185-191.
http://dx.doi.org/10.1179/135100008X308920
---------- VANCOUVER ----------
Miler, E.A., Ríos de Molina, M. del C., Domínguez, G., Guerra, L.N. Thyroid hormone effect in human hepatocytes. Redox Rep. 2008;13(4):185-191.
http://dx.doi.org/10.1179/135100008X308920