Artículo

Franco, D.L.; Nojek, I.M.; Molinero, L.; Coso, O.A.; Costas, M.A. "Osmotic stress sensitizes naturally resistant cells to TNF-α-induced apoptosis" (2002) Cell Death and Differentiation. 9(10):1090-1098
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Abstract:

Most cells are naturally resistant to TNF-α-induced cell death and become sensitized when NF-κB transactivation is blocked or in the presence of protein synthesis inhibitors that prevent the expression of anti-apoptotic genes. In this report we analyzed the role of osmotic stress on TNIF-α-induced cell death. We found that it sensitizes the naturally resistant HeLa cells to TNF-α-induced apoptosis, with the involvement of an increase in the activity of several kinases, the inhibition of Bcl-2 expression, and a late increase on NF-κB activation. Cell death occurs regardless of the enhanced NF-κB activity, whose inhibition produces an increase in apoptosis. The inhibition of p38 kinase, also involved in NF-κB activation, significantly increases the effect of osmotic stress on TNF-α-induced cell death.

Registro:

Documento: Artículo
Título:Osmotic stress sensitizes naturally resistant cells to TNF-α-induced apoptosis
Autor:Franco, D.L.; Nojek, I.M.; Molinero, L.; Coso, O.A.; Costas, M.A.
Filiación:Laboratorio de Fisiología y Biología Molecular, Departamento de Cs. Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón II, C1428EHA Bs. As., Argentina
Laboratorio de Biología Molecular y Apoptosis, Departamento de Sustancias Vasoactivas, Instituto de Investigaciones Médicas A. Lanari, Universidad de Buenos Aires, Combatientes de Malvinas 3150, C1427 Bs. As, Argentina
Laboratorio de Inmunogenética, Hospital Nacional de Clínícas ‘José de San Martin’, Facultad de Medicina, Universidad de Buenos Aires, Argentina
Palabras clave:Apoptosis; MAP kinases; NF-κB; TNF-α; immunoglobulin enhancer binding protein; phosphotransferase; protein bcl 2; protein synthesis inhibitor; synaptophysin; tumor necrosis factor alpha; apoptosis; article; cell death; controlled study; enzyme activity; enzyme inhibition; gene activity; gene expression; HeLa cell; human; human cell; osmotic stress; priority journal; protein synthesis inhibition; sensitization; transactivation; Apoptosis; Dose-Response Relationship, Drug; Enzyme Inhibitors; Eukaryotic Cells; Hela Cells; Humans; MAP Kinase Signaling System; Mitogen-Activated Protein Kinase 12; Mitogen-Activated Protein Kinase 8; Mitogen-Activated Protein Kinases; NF-kappa B; Osmotic Pressure; p38 Mitogen-Activated Protein Kinases; Proto-Oncogene Proteins c-bcl-2; Saline Solution, Hypertonic; Stress; Sulfasalazine; Transcription Factor RelA; Tumor Necrosis Factor-alpha
Año:2002
Volumen:9
Número:10
Página de inicio:1090
Página de fin:1098
DOI: http://dx.doi.org/10.1038/sj.cdd.4401074
Título revista:Cell Death and Differentiation
Título revista abreviado:Cell Death Differ.
ISSN:13509047
CAS:Enzyme Inhibitors; Mitogen-Activated Protein Kinase 12, EC 2.7.1.-; Mitogen-Activated Protein Kinase 8, EC 2.7.1.37; Mitogen-Activated Protein Kinases, EC 2.7.1.37; NF-kappa B; p38 Mitogen-Activated Protein Kinases, EC 2.7.1.37; Proto-Oncogene Proteins c-bcl-2; Saline Solution, Hypertonic; Sulfasalazine, 599-79-1; Transcription Factor RelA; Tumor Necrosis Factor-alpha
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13509047_v9_n10_p1090_Franco

Referencias:

  • Tracey, K.J., Cerami, A., Tumor necrosis factor, other cytokines and disease (1993) Annu. Rev. Cell Biol, 9, pp. 317-343
  • Vandenabeele, P., Declercg, W., Beyaert, R., Fiers, W., Two tumor necrosis factor receptors: Structure and function (1995) Trends Cell Biol, 5, pp. 392-399
  • Tartaglia, L.A., Goeddel, D.V., Two TNF receptors (1992) Immunol. Today, 13, pp. 151-153
  • Rothe, M., Wong, S.C., Henzel, W.J., Goeddel, D.V., A novel family of putative signal transducers associated with the cytoplasmic domain of the 75 kDa tumor necrosis factor receptor (1994) Cell, 78, pp. 681-692
  • Hsu, H., Xiong, J., Goeddel, D.V., The TNF receptor-1-associated protein TRADD signals cell death and NF-κB activation (1995) Cell, 81, pp. 495-504
  • Stanger, B.Z., Leder, P., Lee, T.H., Kim, E., Seed, B., RIP: A novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death (1995) Cell, 81, pp. 513-523
  • Hsu, H., Shu, H.B., Pan, M.G., Goeddel, D.V., TRADD-TRAF2 and TRADD-FADD interactions define two distinct TNF receptor-1 signal transduction pathways (1996) Cell, 84, pp. 299-308
  • Hsu, H., Shu, H.B., Baichwall, V., Goeddel, D.V., TNF-dependent recruitment of the protein kinase RIP to the TNF receptor-1 signaling complex (1996) Immunity, 4, pp. 387-396
  • Liu, Z., Hsu, H., Goeddel, D.V., Karin, M., Dissection of TNF receptor 1 effector functions: JNK activation is not linked to apoptosis while NF-κB activation prevents cell death (1996) Cell, 87, pp. 565-576
  • Wajant, H., Henkler, F., Scheurich, P., The TNF-receptor-associated factor family scaffold molecules for cytokine receptors, kinases and their regulators (2001) Cell Signal, 13, pp. 389-400
  • Gioacchino, N., Costanzo, A., Guido, F., Moretti, A.B., Burgio, V.L., Agresti, C., Levrero, M., Nuclear factor κB-independent cytoprotective pathways originating at tumor necrosis factor receptor-associated factor 2 (1998) J. Biol. Chem, 273, pp. 31262-31272
  • Beg, A., Baltimore, D., An essential role for NF-κB in preventing TNF-α-induced cell death (1996) Science, 274, pp. 782-784
  • Chu, Z.L., McKinsey, T., Liu, L., Gentry, J.J., Malim, M.H., Ballard, D.W., Suppression of tumor necrosis factor-induced cell death by inhibitor of apoptosis c-IAP2 is under NF-κB control (1997) Proc. Natl. Acad. Sci. USA, 94, pp. 10057-10062
  • Wang, C.Y., Mayo, M.W., Baldwin, A.S., TNF-α and cancer therapy-induced apoptosis: Potentiation by inhibition of NF-κB (1996) Science, 274, pp. 784-787
  • Costas, M.A., Muller Igaz, L., Holsboer, F., Arzt, E., Transrepression of NF-κB is not required for glucocorticoid-mediated protection of TNF-α-induced apoptosis on fibroblasts (2000) Biochim. Biophys. Acta, pp. 122-129. , 1499
  • Wang, C., Mayo, M.W., Korneluk, R.G., Goeddel, D.V., Baldwin, A.S., NF-κB antiapoptosis: Induction of TRAF1 and TRAF2 and c-IAP1 and c-IAP2 to suppress caspase-8 activation (1998) Science, 281, pp. 1680-1683
  • Van Antwerp, D.J., Martin, S.J., Kafri, T., Green, D.R., Verma, I.M., Suppression of TNF-α-induced apoptosis by NF-κB (1996) Science, 274, pp. 787-789
  • Larrick, J.W., Wright, S.C., Cytotoxic mechanism of tumor necrosis factor-alpha (1990) FASEB J, 4, pp. 3215-3223
  • Mukhopadhyay, T., Roth, J.A., Maxwell, S.A., Induction of p53 DNA-binding activity by tumor necrosis factor-α (1996) Int. J. Oncol, 9, pp. 715-720
  • Pahl, H.L., Activators and target genes of Rel/NF-κB transcription factors (1999) Oncogene, 18, pp. 6853-6866
  • Lin, B., Williams-Skipp, C., Tao, Y., Schleicher, M.S., Cano, L.L., Duke, R.C., Scheinman, R.I., NF-κB functions as both a proapoptotic and antiapoptotic regulatory factor within a single cell type (1999) Cell Death Differ, 6, pp. 570-582
  • Cobb, M., Robbins, D., Boulton, T., ERKs, extracellular signal-regulated MAP-2-kinases (1991) Curr. Biol, 3, pp. 1025-1032
  • Derijard, B., Hibi, M., Wu, I., Baret, T., Su, B., Deng, T., Karin, M., Davis, R., JNK1: A protein kinase stimulated by UV light and Ha-Ras that binds and phosphorilates the c-Jun activation domain (1994) Cell, 76, pp. 1025-1037
  • Coso, O.A., Chiariello, M., Kalinec, G., Kyriakis, J.M., Woodgett, J., Gutkind, S., Transforming G protein-coupled receptors potently activate JNK (SAPK) (1995) J. Biol. Chem, 270, pp. 5620-5624
  • Coso, O., Chiariello, M., Yu, J., Teramoto, H., Crespo, P., Xu, N., Miki, T., Gutkind, J.S., The small GTP-binding proteins Rac and Cdc42 regulate the activity of the JNK (SAPK) signalling pathway (1995) Cell, 81, pp. 1137-1146
  • Ono, K., Han, J., The p38 signal transduction pathway activation and function (2000) Cellular Signalling, 12, pp. 1-13
  • Nebreda, A.R., Porras, A., P38 MAP kinases: Beyond the stress response (2000) TIBS, 25, pp. 257-260
  • Kültz, D., Burg, M., Evolution of osmotic stress and signalling via MAP kinase cascades (1998) J. Exp, 201, pp. 3015-3021
  • Bode, J.G., Gatsios, P., Ludwig, S., Rapp, U.R., Häussinger, D., Heinrich, P.C., Graeve, L., The mitogen-activated protein (MAP) kinase p38 and its upstream activator MAP kinase 6 are involved in the activation of signal transducer and activator of transcription by hyperosmolarity (1999) J. Biol. Chem, 274, pp. 30222-30227
  • Berghe, W.V., Plaisance, S., Boone, E., De Bosscher, K., Schmitz, M.L., Fiers, W., Haegeman, G., P38 and extracellular signal-regulated kinase mitogen-activated protein kinase pathways are required for nuclear factor-κB p65 transactivation mediated by tumor necrosis factor (1998) J. Biol. Chem, 273, pp. 3285-3290
  • Downward, J., Ras signaling and apoptosis (1998) Curr. Opin. Genet. Dev, 8, pp. 49-54
  • Cuvillier, O., Pirianov, G., Kleuser, B., Vanek, P.G., Coso, O.A., Gutkind, J.S., Spiegel, S., Suppression of ceramide-mediated programmed cell death by sphingosine-1-phosphate (1996) Nature, 381, pp. 800-803
  • Monney, L., Olivier, R., Otter, I., Jansen, B., Poirier, G., Borner, C., Role of an acidic compartment in tumor-necrosis-factor-α-induced production of ceramide, activation of caspase-3 and apoptosis (1998) Eur. J. Biochem, 251, pp. 295-303
  • Green, D., Apoptopic pathways: The roads to ruin (1998) Cell, 94, pp. 695-698
  • Gross, A., McDonnell, J.M., Korsmeyer, S.J., BCL-2 family members and the mitochondria in apoptosis (1999) Genes Dev, 13, pp. 1899-1911
  • Gerritsen, M.E., Williams, A.J., Neish, A.S., Moore, N.S., Shi, Y., Collins, T., CREB-binding protein/p300 are transcriptional co-activators of p65 (1997) Proc. Natl. Acad. Sci. USA, 94, pp. 2927-2932
  • Bortner, C.D., Hughes, F.M., Cidlowski, J.A., A primary role K+ and Na+ efflux in the activation of apoptosis (1997) J. Biol. Chem, 272, pp. 32436-32442
  • Bortner, C.D., Cidlowski, J.A., A necessary role for cell shrinkage in apoptosis (1998) Biochem. Pharmacol, 56, pp. 1549-1559
  • Bortner, C.D., Cidlowski, J.A., Volume regulation and ion transport during apoptosis (2000) Methods Enzymol, 322, pp. 421-433
  • Okada, Y., Maeno, E., Shimizu, T., Dezaki, K., Wang, J., Morishima, S., Receptor-mediated control of regulatory volume decrease (RVD) and apoptopic volume decrease (AVD) (2001) J. Physiol, 532, pp. 3-16
  • Montague, J.W., Bortner, C.D., Hughes, F.M., Cidlowski, J.A., A necessary role for reduced intracellular potassium during the DNA degradation phase of apoptosis (1999) Steroids, 64, pp. 563-569
  • Kültz, D., Madhany, S., Burg, M.B., Hyperosmolality causes growth arrest of murine kidney cells (1998) J. Biol. Chem, 273, pp. 13645-13651
  • Carter, A.B., Knudtson, K.L., Monick, M.M., Hunninghake, G.W., The p38 mitogen-activated protein kinase is required for NF-κB-dependent gene expression (1999) J. Biol. Chem, 274, pp. 30858-30863
  • Polla, B.S., Jacquier-Sarlin, M.R., Kantengwa, S., Mariethoz, E., Hennet, T., Russo-Marie, F., Cossarizza, A., TNF alpha alters mitochondrial membrane potential in L929 but not in TNF alpha-resistant L929. 12 cells: Relationship with the expression of stress proteins, annexin 1 and superoxide dismutase activity (1996) Free Radic. Res, 25, pp. 125-131
  • Albrecht, H., Tschopp, J., Jongeneel, V., Bcl-2 protects from oxidative damage and apoptopic cell death without interferring with activation of NF-κB by TNF (1994) FEBS Letters, 351, pp. 45-48
  • Wahl, C., Liptay, S., Adler, G., Schmid, R., Sulfasalazine: A potent and specific inhibitor of nuclear factor kappa B (1998) J. Clin. Invest, 101, pp. 1163-1174

Citas:

---------- APA ----------
Franco, D.L., Nojek, I.M., Molinero, L., Coso, O.A. & Costas, M.A. (2002) . Osmotic stress sensitizes naturally resistant cells to TNF-α-induced apoptosis. Cell Death and Differentiation, 9(10), 1090-1098.
http://dx.doi.org/10.1038/sj.cdd.4401074
---------- CHICAGO ----------
Franco, D.L., Nojek, I.M., Molinero, L., Coso, O.A., Costas, M.A. "Osmotic stress sensitizes naturally resistant cells to TNF-α-induced apoptosis" . Cell Death and Differentiation 9, no. 10 (2002) : 1090-1098.
http://dx.doi.org/10.1038/sj.cdd.4401074
---------- MLA ----------
Franco, D.L., Nojek, I.M., Molinero, L., Coso, O.A., Costas, M.A. "Osmotic stress sensitizes naturally resistant cells to TNF-α-induced apoptosis" . Cell Death and Differentiation, vol. 9, no. 10, 2002, pp. 1090-1098.
http://dx.doi.org/10.1038/sj.cdd.4401074
---------- VANCOUVER ----------
Franco, D.L., Nojek, I.M., Molinero, L., Coso, O.A., Costas, M.A. Osmotic stress sensitizes naturally resistant cells to TNF-α-induced apoptosis. Cell Death Differ. 2002;9(10):1090-1098.
http://dx.doi.org/10.1038/sj.cdd.4401074