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Abstract:

A new strain of Euglena gracilis Klebs has been isolated from a highly polluted river; it was named MAT. Strain growth in different culture media was evaluated under heterotrophic and autotrophic conditions. Total lipid, sugar, protein and chlorophyll a production were studied. Results obtained for MAT were compared with data obtained for a UTEX Culture Collection strain. Likewise, cells from both strains were bleached using streptomycin, and grown in the same media used for green samples. Both MAT and UTEX showed clear differences in their biochemical composition and growth rate depending on the media used. They also exhibited different growth patterns. E. gracilis medium proved to be the best culture environment for both strains either in autotrophic or heterotrophic conditions. Results show that basal contents of lipids, sugars, proteins and chlorophyll a vary depending on the strain, and thus values obtained for one strain do not apply to another. Moreover, strain origin may have an influence on the mechanisms of adaptation or defense developed by each sample.

Registro:

Documento: Artículo
Título:Isolation, culture and characterization of a new strain of Euglena gracilis
Autor:Ruiz, L.B.; Rocchetta, I.; Dos Santos Ferreira, V.; Conforti, V.
Filiación:Depto. de Biodiversidad Y Biol. Exp., Fac. de Ciencias Exactas Y Naturales, Ciudad Universitaria, Pab. II, 1428, Buenos Aires, Argentina
Palabras clave:Bleached; Culture; Euglena gracilis; Lipid; Protein; Strain; Sugar; flagellate; river pollution; Euglena; Euglena gracilis; Euglena gracilis; Mastigophora (flagellates)
Año:2004
Volumen:52
Número:2
Página de inicio:168
Página de fin:173
DOI: http://dx.doi.org/10.1111/j.1440-1835.2004.tb00325.x
Título revista:Phycological Research
Título revista abreviado:Phycol. Res.
ISSN:13220829
CODEN:PHREF
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13220829_v52_n2_p168_Ruiz

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Citas:

---------- APA ----------
Ruiz, L.B., Rocchetta, I., Dos Santos Ferreira, V. & Conforti, V. (2004) . Isolation, culture and characterization of a new strain of Euglena gracilis. Phycological Research, 52(2), 168-173.
http://dx.doi.org/10.1111/j.1440-1835.2004.tb00325.x
---------- CHICAGO ----------
Ruiz, L.B., Rocchetta, I., Dos Santos Ferreira, V., Conforti, V. "Isolation, culture and characterization of a new strain of Euglena gracilis" . Phycological Research 52, no. 2 (2004) : 168-173.
http://dx.doi.org/10.1111/j.1440-1835.2004.tb00325.x
---------- MLA ----------
Ruiz, L.B., Rocchetta, I., Dos Santos Ferreira, V., Conforti, V. "Isolation, culture and characterization of a new strain of Euglena gracilis" . Phycological Research, vol. 52, no. 2, 2004, pp. 168-173.
http://dx.doi.org/10.1111/j.1440-1835.2004.tb00325.x
---------- VANCOUVER ----------
Ruiz, L.B., Rocchetta, I., Dos Santos Ferreira, V., Conforti, V. Isolation, culture and characterization of a new strain of Euglena gracilis. Phycol. Res. 2004;52(2):168-173.
http://dx.doi.org/10.1111/j.1440-1835.2004.tb00325.x