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Abstract:

Arbuscular mycorrhizal (AM) fungal community associated with pecan (Carya illinoinensis) roots and rhizospheric soils was assessed by spore isolation and morphological characterisation and by pyrosequencing of AM molecular markers. The AM fungal community associated with pecan growing in the field, was always more diverse than that associated with pecan growing in containers. This was not observed when AM richness was studied, suggesting that soil disturbance by a reduction in host plant richness leads to a less equitable distribution of AM fungal species, in contrast to natural soils. The chosen primers (AMV4.5F/AMDGR) for pyrosequencing showed high AM fungal specificity. Based on 97% sequence similarity, 49 operational taxonomic units (MOTUs) were obtained and, amongst these, 41 MOTUs corresponded to the Glomeromycota phylum. The number of obtained AM sequences ranged from 2164, associated with field samples, to 5572 obtained from pecan trap pot culture samples, defining 30 and 29 MOTUs, respectively. Richness estimated by conventional species identification was 6 and 9 AM fungal species in soil and pot samples, respectively. Claroideoglomus lamellosum, Funneliformis mosseae and Entrophospora infrequens were the only taxa detected using both techniques. Predominant sequences in the pecan rhizosphere samples, such as Rhizoglomus irregulare and other less abundant (Dominikia iranica, Dominikia indica, Sclerocystis sinuosa, Paraglomus laccatum), were detected only by pyrosequencing. Detection of AM fungal species based on spore morphology, in combination with molecular approaches, provides a more comprehensive estimate of fungal community composition. Copyright L.F. Bidondo et al.

Registro:

Documento: Artículo
Título:Detection of arbuscular mycorrhizal fungi associated with pecan (Carya illinoinensis) trees by molecular and morphological approaches
Autor:Fernández Bidondo, L.; Colombo, R.P.; Recchi, M.; Silvani, V.A.; Pérgola, M.; Martínez, A.; Godeas, A.M.
Filiación:Laboratorio de Microbiología del Suelo, Instituto de Biodiversidad y Biología Experimental Aplicada, Facul-tad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 4to piso, Pabellón 2., Buenos Aires, 1428, Argentina
Año:2018
Volumen:42
Página de inicio:73
Página de fin:88
DOI: http://dx.doi.org/10.3897/mycokeys.42.26118
Título revista:MycoKeys
Título revista abreviado:MycoKeys
ISSN:13144057
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13144057_v42_n_p73_FernandezBidondo

Referencias:

  • Baldrian, P., Head, I.M., Prosser, J.I., Schloter, M., Smalla, K., Tebbe, C.C., Ecology and metagenomics of soil microorganisms (2011) FEMS Microbiology Ecology, 78, pp. 1-2. , https://doi.org/10.1111/j.1574-6941.2011.01184.x
  • Benucci, G.M., Bonito, G., Baciarelli-Falini, L., Bencivenga, M., Mycorrhization of Pecan trees (Carya illinoinensis) with commercial truffle species: Tuber aestivum Vittad. And Tuber borchii Vittad (2012) Mycorrhiza, 22 (5), pp. 383-392. , https://doi.org/10.1007/s00572-011-0413-z
  • Blaszkowski, J., (2012) The Glomeromycota, pp. 1-297. , Szafer W Publisher. Institute of Botany Polish Academy of Sciences, Kraków
  • Bonito, G., Brenneman, T., Vilgalys, R., Ectomycorrhizal fungal diversity in orchards of cultivated pecan (Carya illinoinensis; Juglandaceae) (2011) Mycorrhiza, 21 (7), pp. 601-612. , https://doi.org/10.1007/s00572-011-0368-0
  • Brock, P.M., Doring, H., Bidartondo, M.I., How to know unknown fungi: The role of a herbar-ium (2009) New Phytologist, 181, pp. 719-724. , https://doi.org/10.1111/j.1469-8137.2008.02703.x
  • Brundrett, M., Murase, G., Kendrick, B., Comparative anatomy of roots and mycorrhizae of common Ontario trees (1990) Canadian Journal of Botany, 68 (3), pp. 551-578. , https://doi.org/10.1139/b90-076
  • Caporaso, J.G., Kuczynski, J., Stombaugh, J., Bittinger, K., Bushman, F.D., Costello, E.K., Fierer, N., Knight, R., QIIME allows analysis of high-throughput community sequencing data (2010) Nature Methods, 7, pp. 335-336. , https://doi.org/10.1038/nmeth.f.303
  • Colombo, R., Fernandez Bidondo, L., Silvani, V., Carbonetto, M.B., Rascován, N., Bompadre, M.J., Pérgola, M., Godeas, A., Diversity of arbuscular mycorrhizal fungi in soil from the Pampa Ondulada, Argentina, assesed by pyrosequencing and traditional techniques (2014) Can. J. Microbiol, 60, pp. 819-827. , https://doi.org/10.1139/cjm-2014-0364
  • Declerck, S., Bivort, C., de Souza, F.A., Development of extraradical mycelium of Scutellospora reticulata under root-organ culture: Spore production and function of auxiliary cells (2004) Mycological Research, 108, pp. 84-92. , https://doi.org/10.1017/S0953756203008761, D’Or D
  • dos Santos, V.L., Muchovej, R.M., Chaer Borges, A., Neves, J.C., Kasuya, M.C.M., Vesicular-arbuscular-/ecto-mycorrhiza succession in seedlings of Eucalyptus spp (2001) Brazilian Journal of Microbiology, 32, pp. 81-86. , https://doi.org/10.1590/S1517-83822001000200002
  • Dumbrell, A.J., Ashton, P.D., Aziz, A., Feng, G., Nelson, M., Dytham, C., Fitter, A.H., Helgason, T., Distinct seasonal assemblage of arbuscular mycorrhizal fungi revealed by massively parallel pyrosequencing (2011) New Phytologist, 190, pp. 794-804. , https://doi.org/10.1111/j.14698137.2010.03636.x
  • Fitter, A.H., Darkness visible: Reflections on underground ecology (2005) Journal of Ecology, 93, pp. 231-243. , https://doi.org/10.1111/j.0022-0477.2005.00990.x
  • Hewitt, E.J., Sand water culture methods used in the study of plant nutrition (1966) Common Wealth Agriculture Bureau, , Technical Communication N°221952
  • Hibbett, D.S., Ohman, A., Glotzer, D., Nuhn, M., Kirk, P., Nilsson, R.H., Progress in molecular and morphological taxon discovery in fungi and options for formal classification of environmental sequences (2011) Fungal Biology Reviews, 25, pp. 38-47. , https://doi.org/10.1016/j.fbr.2011.01.001
  • Husband, R., Herre, E.A., Young, P.W., Temporal variation in the arbuscular mycorrhizal communities colonizing seedlings in a tropical forest (2002) FEMS Microbiology Ecology, 42, pp. 131-136. , https://doi.org/10.1111/j.1574-6941.2002.tb01002.x
  • (1989) Mapa De Suelos De La Provincia De Buenos Aires, , INTA Secretaria de Agricultura, Ga-nadería y Pesca, Proyecto PNUD ARG 85/019. Instituto Nacional de Tecnología Agropecuaria. CIRN-Instituto de Evaluación de Tierras. Buenos Aires, Argentina
  • Konstantinidis, K.T., Tiedje, M., Prokaryotic taxonomy and phylogeny in the genomic era: Advancements and challenges ahead (2007) Current Opinion in Microbiology, 10, pp. 504-509. , https://doi.org/10.1016/j.mib.2007.08.006
  • Lekberg, Y., Schnoor, T., Kjoller, R., Gibbons, S.M., Hansen, L.H., Al-Soud, W.A., Sorensen, S.J., Rosendahl, S., 454-sequencing reveals stochastic local reassembly and high disturbance tolerance within arbuscular mycorrhizal fungal communities (2012) Journal of Ecology, 100, pp. 151-160. , https://doi.org/10.1111/j.1365-2745.2011.01894.x
  • Lin, X., Feng, Y., Zhang, H., Chen, R., Wang, J., Zhang, J., Chu, H., Long-term balanced fertilization decreases arbuscular mycorrhizal fungal diversity in an arable soil in North China revealed by 454 pyrosequencing (2012) Environmental Science Technology, 46 (11), pp. 5764-5771. , https://doi.org/10.1021/es3001695
  • Lumini, E., Orgiazzi, A., Borriello, R., Bonfante, P., Bianciotto, V., Disclosing arbuscular mycorrhizal fungal biodiversity in soil through a land-use gradient using a pyrosequencing approach (2009) Environmental Microbiology, 12, pp. 2165-2179. , https://doi.org/10.1111/j.14622920.2009.02099.x
  • Madero, E., (2013) La Nuez Pecan, , http://anterior.inta.gov.ar/delta/propecan/red.htm
  • Mosse, B., Stribley, D.P., Le Tacon, F., Ecology of mycorrhizae and mycorrhizal fungi (1981) Advance Microbial Ecology, 5, pp. 137-210. , https://doi.org/10.1007/978-1-4615-8306-6_4
  • Muñoz-Márquez, E., Macías-López, C., Franco-Ramírez, A., Sánchez-Chávez, E., Jiménez-Castro, J., González-García, J., Identification and natural colonization of mycorrhizal arbuscular fungi in pecan orchards (2009) Terra Latinoamericana, 27 (4), pp. 355-360
  • Neville, J., Tessier, J.L., Morrison, I., Scarratt, J., Canning, B., Klironomos, J.N., Soil depth distribution of ecto and arbuscular mycorrhizal fungi associated with Populus tremuloides within a 3-year-old boreal forest clear-cut (2002) Applied Soil Ecology, 19 (3), pp. 209-216. , https://doi.org/10.1016/S0929-1393(01)00193-7
  • Opik, M., Moora, M., Liira, J., Zobel, M., Composition of root-colonizing arbuscular mycorrhizal fungal communities in different ecosystems around the globe (2006) Journal of Ecol-Ogy94, pp. 778-790. , https://doi.org/10.1111/j.1365-2745.2006.01136.x
  • Opik, M., Metsis, M., Daniell, T.J., Zobel, M., Moora, M., Large-scale parallel 454 sequencing reveals host ecological group specificity of arbuscular mycorrhizal fungi in a boreonemoral forest (2009) New Phytologist, 184, pp. 424-437. , https://doi.org/10.1111/j.14698137.2009.02920.x
  • Phillips, J.M., Hayman, D.S., Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection (1970) Transactions of The British Mycological Society, 55, pp. 158-160. , https://doi.org/10.1016/S0007-1536(70)80110-3
  • Taber, R.A., Worthington, J.W., Trappe, J.M., Taber, W.A., Mycorrhizal fungi associated with native and improved varieties of Pecan in Texas (1982) Phytopathology, 72 (7), p. 951
  • Tarango, S.H., Macias, B.C., Alarcón, A., Pérez, J., Colonización micorrízica, crecimiento y concentración foliar de nutrimentos en nogal pecanero y pistachero (2004) Agric. Téc. Méx., 30, pp. 191-203
  • Unterseher, M., Jumpponen, A., Opik, M., Tedersoo, L., Moora, M., Dormanns, C.F., Schnittler, M., Species abundance distributions and richness estimations in fungal metagenomic – Lesson learned from community ecology (2011) Molecular Ecology, 20, pp. 275-285. , https://doi.org/10.1111/j.1365-294X.2010.04948.x
  • Van der Heijden, E.W., Differential benefits of arbuscular mycorrhizal and ectomycorrhizal infection of Salix repens (2001) Mycorrhiza, 10, pp. 185-193. , https://doi.org/10.1007/s005720000077
  • Wubet, T., Weiß, M., Kottke, I., Oberwinkler, F., Morphology and molecular diversity of arbuscular mycorrhizal fungi in wild and cultivated yew (Taxus baccata) (2003) Canadian Journal of Botany81, pp. 255-266. , https://doi.org/10.1139/b03-020
  • Xu, L., Ravnskov, S., Larsen, J., Nilsson, R.H., Nicolaisen, M., Soil fungal community structure along a soil health gradient in pea fields examined using deep amplicon sequencing (2012) Soil Biology and Biochemistry, 46, pp. 26-32. , https://doi.org/10.1016/j.soilbio.2011.11.010

Citas:

---------- APA ----------
Fernández Bidondo, L., Colombo, R.P., Recchi, M., Silvani, V.A., Pérgola, M., Martínez, A. & Godeas, A.M. (2018) . Detection of arbuscular mycorrhizal fungi associated with pecan (Carya illinoinensis) trees by molecular and morphological approaches. MycoKeys, 42, 73-88.
http://dx.doi.org/10.3897/mycokeys.42.26118
---------- CHICAGO ----------
Fernández Bidondo, L., Colombo, R.P., Recchi, M., Silvani, V.A., Pérgola, M., Martínez, A., et al. "Detection of arbuscular mycorrhizal fungi associated with pecan (Carya illinoinensis) trees by molecular and morphological approaches" . MycoKeys 42 (2018) : 73-88.
http://dx.doi.org/10.3897/mycokeys.42.26118
---------- MLA ----------
Fernández Bidondo, L., Colombo, R.P., Recchi, M., Silvani, V.A., Pérgola, M., Martínez, A., et al. "Detection of arbuscular mycorrhizal fungi associated with pecan (Carya illinoinensis) trees by molecular and morphological approaches" . MycoKeys, vol. 42, 2018, pp. 73-88.
http://dx.doi.org/10.3897/mycokeys.42.26118
---------- VANCOUVER ----------
Fernández Bidondo, L., Colombo, R.P., Recchi, M., Silvani, V.A., Pérgola, M., Martínez, A., et al. Detection of arbuscular mycorrhizal fungi associated with pecan (Carya illinoinensis) trees by molecular and morphological approaches. MycoKeys. 2018;42:73-88.
http://dx.doi.org/10.3897/mycokeys.42.26118