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Abstract:

Background: Previously we showed that corticosterone and aldosterone increased proton fluxes in proximal tubule, by micropuncture and stationary microperfusion. Since the Na+/H+ exchanger is responsible for the main proximal proton secretion, we have now evaluated the effects aldosterone on Na+/H+ exchange activity in brush border vesicles. In order to evaluate the mechanism of action of glucocorticoids and mineralocorticoids, we studied the comparative effects of corticosterone and aldosterone on the abundance of NHE3 and NHE2 isoforms. Material/Methods: We isolated renal brush border vesicles from rats by differential centrifugation in sham-operated, adrenalectomized, and adrenalectomized-aldosterone treated (ADX + aldosterone) animals. We measured the kinetics of H+ transport in response to increasing concentrations of Sodium Gluconate by fluorimetry using acridine orange. For Na+/H+ exchanger abundance we used Western blot analysis of brush border proteins in the above groups and in adrenalectomized-corticosterone treated rats. Results: The Vmax in adrenalectomized animals was 22,162±1828 fluorescence units/min; in sham animals, 37,020±2722; and in ADX + aldosterone, 42,344±3044 (p<0.01 adrenalectomized vs others). No differences in Km were observed. Adrenalectomy decreased NHE3 abundance over Sham by 32% without modifying NHE2. Corticosterone-replacement enhanced NHE3 abundance by 76% and failed to increase NHE2. Aldosterone enhanced NHE2 abundance by 75% and did not increase NHE3. Conclusion: Mineralocorticoids enhance Na+/H+ exchange activity by increasing NHE2 abundance; glucocorticoids, by increasing NHE3 abundance.

Registro:

Documento: Artículo
Título:The mechanisms of brush border Na+/H+ exchanger activation by corticosteroids
Autor:Zallocchi, M.; Igarreta, P.; Calvo, J.C.; Reboucas, N.A.; Damasco, M.C.
Filiación:Department of Biochemistry, FCEN, U.B.A, Buenos Aires, Argentina
Instituto de Ciencias Biomedicas, USP, Sao Paulo, Brazil
CONICET, Buenos Aires, Argentina
Dep. Quimica Biologica, Pabellón 2, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Palabras clave:Aldosterone; Glucocorticoids; H+ transport; NHE2; NHE3; acridine orange; aldosterone; corticosteroid; corticosterone; gluconate sodium; mineralocorticoid; sodium proton exchange protein; article; brush border; fluorometry; kidney proximal tubule; molecular dynamics; molecular mechanics; proton transport; receptor affinity; transport kinetics; upregulation; Western blotting; Adrenalectomy; Aldosterone; Animals; Anti-Inflammatory Agents; Corticosterone; Gluconates; Kidney Tubules, Proximal; Male; Microvilli; Protein Isoforms; Rats; Rats, Sprague-Dawley; Sodium-Hydrogen Antiporter
Año:2003
Volumen:9
Número:2
Página de inicio:BR85
Página de fin:BR90
Título revista:Medical Science Monitor
Título revista abreviado:Med. Sci. Monit.
ISSN:12341010
CODEN:MSMOF
CAS:acridine orange, 494-38-2, 65-61-2; aldosterone, 52-39-1, 6251-69-0; corticosterone, 50-22-6; gluconate sodium, 527-07-1; sodium proton exchange protein, 204597-32-0; Aldosterone, 52-39-1; Anti-Inflammatory Agents; Corticosterone, 50-22-6; Gluconates; gluconic acid, 526-95-4; Protein Isoforms; Slc9a2 protein, rat; Sodium-Hydrogen Antiporter; sodium-hydrogen exchanger 3
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_12341010_v9_n2_pBR85_Zallocchi

Referencias:

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Citas:

---------- APA ----------
Zallocchi, M., Igarreta, P., Calvo, J.C., Reboucas, N.A. & Damasco, M.C. (2003) . The mechanisms of brush border Na+/H+ exchanger activation by corticosteroids. Medical Science Monitor, 9(2), BR85-BR90.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_12341010_v9_n2_pBR85_Zallocchi [ ]
---------- CHICAGO ----------
Zallocchi, M., Igarreta, P., Calvo, J.C., Reboucas, N.A., Damasco, M.C. "The mechanisms of brush border Na+/H+ exchanger activation by corticosteroids" . Medical Science Monitor 9, no. 2 (2003) : BR85-BR90.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_12341010_v9_n2_pBR85_Zallocchi [ ]
---------- MLA ----------
Zallocchi, M., Igarreta, P., Calvo, J.C., Reboucas, N.A., Damasco, M.C. "The mechanisms of brush border Na+/H+ exchanger activation by corticosteroids" . Medical Science Monitor, vol. 9, no. 2, 2003, pp. BR85-BR90.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_12341010_v9_n2_pBR85_Zallocchi [ ]
---------- VANCOUVER ----------
Zallocchi, M., Igarreta, P., Calvo, J.C., Reboucas, N.A., Damasco, M.C. The mechanisms of brush border Na+/H+ exchanger activation by corticosteroids. Med. Sci. Monit. 2003;9(2):BR85-BR90.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_12341010_v9_n2_pBR85_Zallocchi [ ]