Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

A novel chromosome complement (2n = 14 = 12 + XY/XX; male/female sex chromosomes), male meiosis behaviour, heterochromatin characterization, and frequency and distribution of chiasmata are described for the first time in specimens from a natural population of the giant water bug, Belostoma candidulum Montandon, 1903 (Heteroptera: Belostomatidae) from Argentina. To date, specimens of B. candidulum have been reported by other authors in a sample from a natural population from Brazil. Our results demonstrate that Argentinean and Brazilian populations have different diploid numbers and chromosomal features. During male meiosis, autosomal bivalents generally show a single chiasma, behave as telokinetic chromosomes (i.e. kinetic activity is restricted to terminal regions), and divide reductionally at anaphase I; in contrast, the sex chromosomes are achiasmatic, behave as univalents and segregate equationally at anaphase I. Among autosomal bivalents of B. candidulum, one is remarkably larger and may present one or two terminal chiasmata, showing rod, V-shaped and ring configurations. Here we propose a new mode of segregation for ring bivalents, since it is not essential that one of the chiasmata is released during anaphase I because alternative sites for microtubule attachment become functional for the normal chromosome segregation to the poles. Heterochromatin content is very scarce in specimens from Argentinean B. candidulum populations, revealing C-positive interstitial and terminal dots in three pairs of autosomes and C-blocks at both ends of X chromosome, whereas the Y chromosome is mainly C-positive. One of the C-positive bands from X and Y chromosomes is DAPIdull/ CMA-bright, which could represent the nucleolus organizing region (NOR) detected by fluorescent in situ hybridization (FISH) The location of the NORs in both sex chromosomes allowed us to use them as a cytological marker to describe their behaviour during meiosis. Despite the fact that specimens from the Argentinean and Brazilian populations have been classified as a single species due to their morphological similarity, our results suggest that both populations are chromosomal races or even morphologically-identical cryptic species. The results obtained support the hypothesis that karyotype of B. candidulum originated through autosomal fusions and the fusion of the X and Y chromosomes with the ancestral NOR-autosomal pair. Lastly, the genus Belostoma represents an excellent model for assessing the main mechanisms involved in the karyotype evolution in organisms with holokinetic chromosomes, from which inferences may be made concerning its broader ecology and evolution.

Registro:

Documento: Artículo
Título:Karyotype evolution in progress: A new diploid number in Belostoma candidulum (Heteroptera: Belostomatidae) from Argentina leading to new insights into its ecology and evolution
Autor:Chirino, M.G.; Bressa, M.J.
Filiación:Instituto de Ecología, Genética y Evolución de Buenos Aires, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón II, C1428EHA Ciudad Autónoma de Buenos Aires, Argentina
Laboratorio de Entomología Aplicada y Forense, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Sáenz Peña 352, B1876BXD Bernal, Buenos Aires, Argentina
Palabras clave:Belostoma candidulum; Belostomatidae; Chromosomal races; Cryptic species; Heteroptera; Holokinetic chromosomes; Karyotype evolution; RDNA-FISH; Ring bivalents; chromosome; DNA; hybridization; karyotype; morphology; Argentina; Brazil
Año:2014
Volumen:111
Número:2
Página de inicio:165
Página de fin:174
DOI: http://dx.doi.org/10.14411/eje.2014.027
Título revista:European Journal of Entomology
Título revista abreviado:Eur. J. Entomol.
ISSN:12105759
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_12105759_v111_n2_p165_Chirino

Referencias:

  • (1998) Statistix for Windows., , Analytycal Software Tallahassee, FL, USA Version 2.0
  • Bardella, V.B., Dias, A.L., Giuliano-Caetano, L., Ribeiro, J.R.I., Da Rosa, R., Sex chromosome differentiation in Belostoma (Insecta: Heteroptera: Belostomatidae) (2012) Genet. Mol. Res, 11, pp. 2476-2486
  • Bressa, M.J., Papeschi, A.G., Mola, L.M., Larramendy, M.L., Meiotic studies in Largus rufipennis (Castelnau) (Largidae Heteroptera). II. Reciprocal translocation heterozygosity. (1998) Caryologia, 51, pp. 253-264
  • Bressa, M.J., Papeschi, A.G., Mola, L.M., Larramendy, M.L., Meiotic studies in Dysdercus Guérin Meneville 1831 (Heteroptera: Pyrrhocoridae). I. Neo-XY in Dysdercus albofasciatus Berg 1878, a new sex chromosome determining system in Heteroptera (1999) Chromosome Res, 7, pp. 503-508
  • Bressa, M.J., Papeschi, A.G., Mola, L.M., Larramendy, M.L., Autosomal univalents as a common meiotic feature in Jadera haematoloma (Herrich-Schaeffer, 1847) and Jadera sanguinolenta (Fabricius, 1775) (Heteroptera Rhopalidae: Rhopalinae). (2001) Eur. J. Entomol., 98, pp. 151-157
  • Bressa, M.J., Papeschi, A.G., Mola, L.M., Larramendy, M.L., Meiotic studies in Lygaeus alboornatus Blanchard 1852 (Heteroptera: Lygaeidae: Lygaeinae) (2002) Caryologia, 55, pp. 15-19
  • Bressa, M.J., Fumagalli, E., Ituarte, S., Frassa, M.V., Larramendy, M.L., Meiotic studies in Dysdercus Guérin Méneville 1831 (Heteroptera: Pyrrhocoridae). II. Evidence of variations in the diffuse stage between wild and laboratory inbred populations of Dysdercus chaquensis Freiberg (2002) Hereditas, 137, pp. 125-131
  • Bressa, M.J., Larramendy, M.L., Papeschi, A.G., Heterochromatin characterization in five species of Heteroptera (2005) Genetica, 124, pp. 307-317
  • Bressa, M.J., Papeschi, A.G., Vítková, M., Kubíčková, S., Fuková, I., Pigozzi, M.I., Marec, F., Sex chromosome evolution in cotton stainers of the genus Dysdercus (Heteroptera: Pyrrhocoridae) (2009) Cytogenet. Genome Res, 125, pp. 292-305
  • Camacho, J.P.M., Belda, J., Cabrero, J., Meiotic behaviour of the holocentric chromosomes of Nezara viridula (Insecta Heteroptera) analysed by C-banding and silver impregnation. (1985) Can. J. Genet. Cytol., 27, pp. 490-497
  • Cattani, M.V., Papeschi, A.G., Nucleolus organizing regions and semi-persistent nucleolus during meiosis in Spartocera fusca (Thunberg) (Coreidae Heteroptera). (2004) Hereditas, 140, pp. 105-111
  • Cattani, M.V., Greizerstein, E.J., Papeschi, A.G., Male meiotic behaviour and nucleolus organizer regions in Camptischium clavipes (Fabr.) (Coreidae Heteroptera) analyzed by fluorescent banding and in situ hybridization. (2004) Caryologia, 57, pp. 267-273
  • Chirino, M.G., Papeschi, A.G., Bressa, M.J., The significance of cytogenetics for the study of karyotype evolution and taxonomy of water bugs (Heteroptera Belostomatidae) native to Argentina. (2013) Comp. Cytogenet., 7, pp. 9-27
  • Criniti, A., Simonazzi, G., Cassanelli, S., Ferrari, M., Bizzaro, D., Manicardi, G.C., Distribution of heterochromatin and rDNA on the holocentric chromosomes of the aphids Dysaphis plantaginea and Melanaphis pyraria (Hemiptera: Aphididae) (2009) Eur. J. Entomol., 106, pp. 153-157
  • Daniel, W.W., (1990) Applied Nonparametric Statistics, p. 635. , PWSKent, Devon, UK
  • Franco, M., Bressa, M.J., Papeschi, A.G., Karyotype and male meiosis in Spartocera batatas (Fabricius) and meiotic behaviour of multiple sex chromosomes in Coreidae (2006) Heteroptera. Eur. J. Entomol., 103, pp. 9-16
  • Fuková, I., Nguyen, P., Marec, F., Codling moth cytogenetics: karyotype, chromosomal location of rDNA, and molecular differentiation of sex chromosomes (2005) Genome, 48, pp. 1083-1092
  • Glaser, R.W., Ringer solutions and some notes on the physiological basis of their ionic composition (1917) Comp. Biochem. Physiol., 2, pp. 241-289
  • Heckman, C.W., Belostomatidae Encyclopedia of South American Aquatic Insects: Hemiptera - Heteroptera. (2011) Illustrated Keys to Known Families, Genera, and Species in South America, pp. 393-434. , Springer, Dordrecht, Heidelberg, London, New York
  • Jacobs, D.H., Groenveld, H.T., Cytogenetics and karyotype evolution of the genus Silvacoris Jacobs (Heteroptera: Aradidae: Carventinae) with a description of a statistical method to compare karyotypes (2002) Cytologia, 67, pp. 159-168
  • Jacobs, D.H., Liebenberg, H., Cytogenetics of Adamanotus uncotibialis Jacobs (Heteroptera: Aradidae) (2001) Caryologia, 54, pp. 83-86
  • Lockwood, A.P.M., 'Ringer' solutions and some notes on the physiological basis of their ionic composition (1961) Comp. Biochem. Physiol., 2, pp. 241-289
  • Manna, G.K., Chromosomes in evolution in Heteroptera (1984) Chromosomes in Evolution of Eukaryotic Groups, pp. 189-225. , Sharma A.K. & Sharma A. (eds) CRC Press, Boca Raton, FL
  • McClung, C.E., The chromosome complex of orthopteran spermatocytes (1905) Biol. Bull., 9, pp. 304-340
  • Mola, L.M., Papeschi, A.G., Meiotic studies in Largus rufipennis (Castelnau) (Largidae, Heteroptera): frequency and behaviour of ring bivalents, univalents and B chromosomes (1993) Heredity, 71, pp. 33-40
  • Motzko, D., Ruthmann, A., Spindle membranes in mitosis and meiosis of the heteropteran insect Dysdercus intermedius A study of the interrelationship of spindle architecture and the kinetic organization of chromosomes. (1984) Eur. J. Cell Biol., 33, pp. 205-216
  • Papeschi, A.G., C- banding and DNA content in three species of Belostoma (Heteroptera) with large differences in chromosome size and number (1988) Genetica, 76, pp. 43-51
  • Papeschi, A.G., DNA content and heterochromatin variation in species of Belostoma (Heteroptera Belostomatidae). (1991) Hereditas, 115, pp. 109-114
  • Papeschi, A.G., (1992) Estudios Citogenéticos y Evolutivos en Heteroptera, p. 259. , Doctoral Thesis, Universidad de Buenos Aires [in Spanish]
  • Papeschi, A.G., Chromosome rearrangements in Belostoma plebejum (Stål) (Belostomatidae Heteroptera). (1994) Caryologia, 47, pp. 223-230
  • Papeschi, A.G., Correspondence between C-banding and Ag-NOR in the sex chromosomes of Belostoma oxyurum (Belostomatidae Heteroptera). (1995) Cytologia, 60, pp. 291-295
  • Papeschi, A.G., Sex chromosome polymorphism in species of Belostoma (Belostomatidae Heteroptera). (1996) Hereditas, 124, pp. 269-274
  • Papeschi, A.G., Bidau, C.J., Chromosome complement and male meiosis in four species of Belostoma Latreille (Heteroptera: Belostomatidae) (1985) Braz. J. Genet., 8, pp. 249-261
  • Papeschi, A.G., Bressa, M.J., Evolutionary cytogenetics in Heteroptera (2006) J. Biol. Res., 5, pp. 3-21
  • Papeschi, A.G., Mola, L.M., Bressa, M.J., Greizerstein, E.J., Lia, V., Polhemus, L., Behaviour of ring bivalents in holokinetic systems: alternative sites of spindle attachment in Pachylis argentinus and Nezara viridula (Heteroptera) (2003) Chromosome Res, 11, pp. 725-733
  • Pérez, R., Calleros, L., Rose, V., Lorca, M., Panzera, F., Cytogenetic studies on Mepraia gajardoi (Heteroptera: Reduviidae) Chromosome behaviour in a spontaneous translocation mutant. (2004) Eur. J. Entomol., 101, pp. 211-218
  • Poggio, M.G., Bressa, M.J., Papeschi, A.G., Male meiosis, heterochromatin characterization and chromosomal location of rDNA in Microtomus lunifer (Berg 1900) (Hemiptera: Reduviidae: Hammacerinae). (2011) Comp. Cytogenet., 5, pp. 1-22
  • Polhemus, J.T., Polhemus, D.A., Global diversity of true bugs (Heteroptera: Insecta) in freshwater (2008) Hydrobiologia, 595, pp. 379-391
  • Rebagliati, P.J., Mola, L.M., Meiotic behavior and karyotypic variation in Acledra (Pentatomidae Heteroptera). (2010) Genet. Mol. Res., 9, pp. 739-749
  • Rebagliati, P.J., Mola, L.M., Papeschi, A.G., Karyotype and meiotic behaviour of the holokinetic chromosomes of six Argentine species of Pentatomidae (Heteroptera) (2001) Caryologia, 54, pp. 339-347
  • Rebagliati, P.J., Papeschi, A.G., Mola, L.M., Meiosis and fluorescent bandings in Edessa meditabunda and Edessa rufomarginata (Heteroptera: Pentatomidae: Edessinae) (2003) Eur. J. Entomol., 100, pp. 11-18
  • Reeves, A., Tear, J., (2000) MicroMeasure for Windows, version 3.3, , http://www.colostate.edu/Depts/Biology/Micro-Measure), (Free program, distributed by the authors via
  • Ribeiro, J.R., A review of the species of Belostoma Latreille 1807 (Hemiptera: Heteroptera: Belostomatidae) from the four southeastern Brazilian states. (2007) Zootaxa, 1477, pp. 1-70
  • Ribeiro, J.R., Estévez, A.K., The small species of Belostoma Latreille (Heteroptera, Belostomatidae). III. A revision of oxyurum group, with a new species from Brazil and description of the male of B. noualhieri Montandon (2009) Rev. Bras. Entomol., 53, pp. 207-215
  • Sáez, F.A., El empleo de la hematoxilina acética o propiónica para el estudio de los cromosomas con la técnica de aplastamiento (1960) Com. Soc. Biol. Montevideo, p. 1. , (Mimeographed)
  • Sahara, K., Marec, F., Traut, W., TTAGG telomeric repeats in chromosomes of some insects and other arthropods (1999) Chromosome Res, 7, pp. 449-460
  • Schnack, J.A., Los Belostomatidae de la República Argentina (Hemiptera) (1976) Fauna de Agua Dulce de la República Argentina, pp. 1-66. , Ringuelet R.A. (ed.) Fundación para la Educación la Ciencia y la Cultura, Buenos Aires
  • Suja, J.A., Del Cerro, A.L., Page, J., Rufas, J.S., Santos, J.L., Meiotic sister chromatid cohesion in holocentric sex chromosomes of three heteropteran species is maintained in absence of axial elements (2000) Chromosoma, 109, pp. 35-43
  • Thomas, D.B.J., Chromosome evolution in the Heteroptera (Hemiptera): agmatoploidy versus aneuploidy (1987) Ann. Entomol. Soc. Am., 80, pp. 720-730
  • Traut, W., Pachytene mapping in the female silkworm Bombyx mori L (Lepidoptera). (1976) Chromosoma, 58, pp. 275-284
  • Ueshima, N., Hemiptera II: Heteroptera (1979) Animal Cytogenetics Insecta 6, 3, pp. v+117. , John B. (ed.) Borntraeger, Berlin

Citas:

---------- APA ----------
Chirino, M.G. & Bressa, M.J. (2014) . Karyotype evolution in progress: A new diploid number in Belostoma candidulum (Heteroptera: Belostomatidae) from Argentina leading to new insights into its ecology and evolution. European Journal of Entomology, 111(2), 165-174.
http://dx.doi.org/10.14411/eje.2014.027
---------- CHICAGO ----------
Chirino, M.G., Bressa, M.J. "Karyotype evolution in progress: A new diploid number in Belostoma candidulum (Heteroptera: Belostomatidae) from Argentina leading to new insights into its ecology and evolution" . European Journal of Entomology 111, no. 2 (2014) : 165-174.
http://dx.doi.org/10.14411/eje.2014.027
---------- MLA ----------
Chirino, M.G., Bressa, M.J. "Karyotype evolution in progress: A new diploid number in Belostoma candidulum (Heteroptera: Belostomatidae) from Argentina leading to new insights into its ecology and evolution" . European Journal of Entomology, vol. 111, no. 2, 2014, pp. 165-174.
http://dx.doi.org/10.14411/eje.2014.027
---------- VANCOUVER ----------
Chirino, M.G., Bressa, M.J. Karyotype evolution in progress: A new diploid number in Belostoma candidulum (Heteroptera: Belostomatidae) from Argentina leading to new insights into its ecology and evolution. Eur. J. Entomol. 2014;111(2):165-174.
http://dx.doi.org/10.14411/eje.2014.027