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Abstract:

Molecular analysis of a genomic region of Bacillus megaterium, a polyhydroxybutyrate (PHB)-producing microorganism, revealed the presence of a gene coding for the enzyme phosphotransbutyrylase (Ptb). Enzyme activity was measured throughout the different growth phases of B. megaterium and was found to correlate with PHB accumulation during the late-exponential growth phase. Ptb expression was repressed by glucose and activated by the branched amino acids isoleucine and valine. Overexpression of ActBm, a σ 54 regulator from B. megaterium whose gene is located upstream from ptb, caused an increase in Ptb activity and PHB accumulation in B. megaterium.

Registro:

Documento: Artículo
Título:Evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in Bacillus megaterium
Autor:Vazquez, G.J.; Pettinari, M.J.; Méndez, B.S.
Filiación:Depto. de Quím. Biol., Universidad de Buenos Aires, Fac. de Ciencias Exactas y Naturales, Buenos Aires, Argentina
Microbiology Laboratory, Brookhaven National Laboratory, Environmental Sciences Department, Upton, NY, United States
Palabras clave:Bacillus megaterium; Polyhydroxybutyrate Phosphotransbutyrylase; enzyme; glucose; isoleucine; phosphotransbutyrylase; poly(3 hydroxybutyric acid); unclassified drug; valine; article; Bacillus megaterium; bacterial growth; controlled study; correlation analysis; enzyme activity; gene location; genetic code; growth curve; nonhuman; protein expression; Bacillus megaterium; Bacteria (microorganisms)
Año:2003
Volumen:6
Número:2
Página de inicio:127
Página de fin:129
DOI: http://dx.doi.org/10.1007/s10123-003-0120-5
Título revista:International Microbiology
Título revista abreviado:Int. Microbiol.
ISSN:11396709
CAS:glucose, 50-99-7, 84778-64-3; isoleucine, 7004-09-3, 73-32-5; poly(3 hydroxybutyric acid), 26063-00-3; valine, 7004-03-7, 72-18-4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_11396709_v6_n2_p127_Vazquez

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Citas:

---------- APA ----------
Vazquez, G.J., Pettinari, M.J. & Méndez, B.S. (2003) . Evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in Bacillus megaterium. International Microbiology, 6(2), 127-129.
http://dx.doi.org/10.1007/s10123-003-0120-5
---------- CHICAGO ----------
Vazquez, G.J., Pettinari, M.J., Méndez, B.S. "Evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in Bacillus megaterium" . International Microbiology 6, no. 2 (2003) : 127-129.
http://dx.doi.org/10.1007/s10123-003-0120-5
---------- MLA ----------
Vazquez, G.J., Pettinari, M.J., Méndez, B.S. "Evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in Bacillus megaterium" . International Microbiology, vol. 6, no. 2, 2003, pp. 127-129.
http://dx.doi.org/10.1007/s10123-003-0120-5
---------- VANCOUVER ----------
Vazquez, G.J., Pettinari, M.J., Méndez, B.S. Evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in Bacillus megaterium. Int. Microbiol. 2003;6(2):127-129.
http://dx.doi.org/10.1007/s10123-003-0120-5