Artículo

Caballero, V.J.; Mendieta, J.R.; Giudici, A.M.; Crupkin, A.C.; Barbeito, C.G.; Ronchi, V.P.; Chisari, A.N.; Conde, R.D. "Alternation between dietary protein depletion and normal feeding cause liver damage in mouse" (2011) Journal of Physiology and Biochemistry. 67(1):43-52
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Abstract:

The effect of frequent protein malnutrition on liver function has not been intensively examined. Thus, the effects of alternating 5 days of a protein and amino acid-free diet followed by 5 days of a complete diet repeated three times (3 PFD-CD) on female mouse liver were examined. The expression of carbonic anhydrase III (CAIII), fatty acid synthase (FAS), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and glutathione S-transferase P1 (GSTP1) in liver were assessed by proteomics, reverse transcriptase-polymerase chain reaction and Northern blotting. The activities of liver GSTs, glutathione reductase (GR) and catalase (CAT), as well as serum glutamic-oxaloacetic transaminase (SGOT) and glutamic-pyruvic transaminase (SGPT) were also tested. Additionally, oxidative damage was examined by measuring of protein carbonylation and lipid peroxidation. Liver histology was examined by light and electron microscopy. Compared with control mice, 3 PFD-CD increased the content of FAS protein (+90%) and FAS mRNA (+30%), while the levels of CAIII and CAIII mRNAs were decreased (-48% and -64%, respectively). In addition, 3 PFD-CD did not significantly change the content of GSTP1 but produced an increase in its mRNA level (+20%), while it decreased the activities of both CAT (-66%) and GSTs (-26%). After 3 PFD-CD, liver protein carbonylation and lipid peroxidation were increased by +55% and +95%, respectively. In serum, 3 PFD-CD increased the activities of both SGOT (+30%) and SGPT (+61%). In addition, 3 PFD-CD showed a histological pattern characteristic of hepatic damage. All together, these data suggest that frequent dietary amino acid deprivation causes hepatic metabolic and ultrastructural changes in a fashion similar to precancerous or cancerous conditions. © University of Navarra 2010.

Registro:

Documento: Artículo
Título:Alternation between dietary protein depletion and normal feeding cause liver damage in mouse
Autor:Caballero, V.J.; Mendieta, J.R.; Giudici, A.M.; Crupkin, A.C.; Barbeito, C.G.; Ronchi, V.P.; Chisari, A.N.; Conde, R.D.
Filiación:Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas Y Naturales, Universidad Nacional de Mar del Plata-CONICET, Funes 3250 4 nivel, CP 7600 Mar del Plata, Argentina
Departamento de Ciencias Marinas, Facultad de Ciencias Exactas Y Naturales, Universidad Nacional de Mar del Plata, Funes 3350, CP 7600 Mar del Plata, Argentina
Laboratorio de Histología Y Embriología, Escuela de Ciencias Veterinarias, Universidad Nacional de la Plata, Calle 60 y 118, CP 1900 La Plata, Argentina
Instituto de Investigaciones Biológicas, Universidad Nacional de Mar del Plata-CONICET, CC 1245, Mar del Plata CP 7600 Buenos Aires, Argentina
Palabras clave:Cycles of protein depletion; Liver damage; Mouse liver; Oxidative stress; alanine aminotransferase; aspartate aminotransferase; carbonate dehydratase III; catalase; fatty acid synthase; glutathione reductase; glutathione transferase P1; glyceraldehyde 3 phosphate dehydrogenase; messenger RNA; alanine aminotransferase blood level; animal experiment; article; aspartate aminotransferase blood level; controlled study; electron microscopy; female; lipid peroxidation; liver function; liver histology; liver injury; mouse; nonhuman; Northern blotting; protein carbonylation; protein expression; protein malnutrition; proteomics; reverse transcription polymerase chain reaction; Mus
Año:2011
Volumen:67
Número:1
Página de inicio:43
Página de fin:52
DOI: http://dx.doi.org/10.1007/s13105-010-0047-1
Título revista:Journal of Physiology and Biochemistry
Título revista abreviado:J. Physiol. Biochem.
ISSN:11387548
CODEN:JPBIF
CAS:alanine aminotransferase, 9000-86-6, 9014-30-6; aspartate aminotransferase, 9000-97-9; catalase, 9001-05-2; fatty acid synthase, 9045-77-6; glutathione reductase, 9001-48-3; glyceraldehyde 3 phosphate dehydrogenase, 9001-50-7
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_11387548_v67_n1_p43_Caballero

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Citas:

---------- APA ----------
Caballero, V.J., Mendieta, J.R., Giudici, A.M., Crupkin, A.C., Barbeito, C.G., Ronchi, V.P., Chisari, A.N.,..., Conde, R.D. (2011) . Alternation between dietary protein depletion and normal feeding cause liver damage in mouse. Journal of Physiology and Biochemistry, 67(1), 43-52.
http://dx.doi.org/10.1007/s13105-010-0047-1
---------- CHICAGO ----------
Caballero, V.J., Mendieta, J.R., Giudici, A.M., Crupkin, A.C., Barbeito, C.G., Ronchi, V.P., et al. "Alternation between dietary protein depletion and normal feeding cause liver damage in mouse" . Journal of Physiology and Biochemistry 67, no. 1 (2011) : 43-52.
http://dx.doi.org/10.1007/s13105-010-0047-1
---------- MLA ----------
Caballero, V.J., Mendieta, J.R., Giudici, A.M., Crupkin, A.C., Barbeito, C.G., Ronchi, V.P., et al. "Alternation between dietary protein depletion and normal feeding cause liver damage in mouse" . Journal of Physiology and Biochemistry, vol. 67, no. 1, 2011, pp. 43-52.
http://dx.doi.org/10.1007/s13105-010-0047-1
---------- VANCOUVER ----------
Caballero, V.J., Mendieta, J.R., Giudici, A.M., Crupkin, A.C., Barbeito, C.G., Ronchi, V.P., et al. Alternation between dietary protein depletion and normal feeding cause liver damage in mouse. J. Physiol. Biochem. 2011;67(1):43-52.
http://dx.doi.org/10.1007/s13105-010-0047-1