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Abstract:

Background Two-phase olive-mill wastes (or “alperujo”) exhibit highly phytotoxic properties, mainly due to phenols. A valuable option for alperujo is its agricultural use, provided that no phytotoxic effects occur. Aims The present investigation was aimed at evaluating the efficacy of two strains of the lignin-degrading fungus Flammulina velutipes to colonize alperujo in order to produce edible mushrooms and to achieve its detoxification. Methods Some important cultural characters related to mushroom production (earliness, biological efficiency and quality of basidiomes) were estimated. The production of lignocellulolytic enzymes, phenol removal and detoxification of the substrate was evaluated. Results High biological efficiencies (70.8%) were obtained at 12 °C with F. velutipes strain BAFC 670/06 in a substrate containing poplar wood shavings and 90% of alperujo. The nature of the substrate did not seem to exert an important influence on pileus and stem morphology; nevertheless shortest stems were observed at higher temperatures. Endo-β-1,4-glucanase, endo-β-1,4-xylanase, laccase and Mn-peroxidase activities were detected in the extracts recovered from the solid-state cultures. Both F. velutipes strains were effective in removing the phenolic compounds. The initial concentration in the substrate with 90% alperujo was reduced in the case of F. velutipes BAFC 1763 by 84.31%, and 40.15% by F. velutipes BAFC 670/06. Germinability experiments on Raphanus sativus, showed that alperujo phytotoxicity was significantly reduced by F. velutipes cultures. Conclusions The experimented changes by the spent mushroom substrate resulting from F. velutipes cultivation with high amount of alperujo would allow its reuse for agricultural purposes. © 2016 Asociación Española de Micología

Registro:

Documento: Artículo
Título:Flammulina velutipes: An option for “alperujo” use
Autor:Rugolo, M.; Levin, L.; Lechner, B.E.
Filiación:Laboratorio de Micología, PROPLAME-PRHIDEB-CONICET, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428, CABA, Buenos Aires, Argentina
Palabras clave:Alperujo; Flammulina velutipes; Lignocellulolytic enzymes; Mushroom production; cellulase; endo beta 1,4 xylanase; fungal enzyme; laccase; lignocellulolytic enzyme; manganese peroxidase; phenol; unclassified drug; phenol derivative; agricultural waste; Article; concentration (parameters); detoxification; edible mushroom; enzyme activity; enzyme substrate; enzyme synthesis; Flammulina velutipes; fungal colonization; fungal strain; fungal structures; fungus culture; germination; nonhuman; phytotoxicity; radish; temperature; waste management; Agaricales; agriculture; Flammulina; olive tree; physiology; procedures; Agaricales; Agriculture; Flammulina; Olea; Phenols
Año:2016
Volumen:33
Número:4
Página de inicio:242
Página de fin:247
DOI: http://dx.doi.org/10.1016/j.riam.2015.12.001
Título revista:Revista Iberoamericana de Micologia
Título revista abreviado:Rev. Iberoam. Micol.
ISSN:11301406
CODEN:RIBME
CAS:cellulase, 9012-54-8; laccase, 80498-15-3; manganese peroxidase, 114995-15-2; phenol, 108-95-2, 3229-70-7; Phenols
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_11301406_v33_n4_p242_Rugolo

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Citas:

---------- APA ----------
Rugolo, M., Levin, L. & Lechner, B.E. (2016) . Flammulina velutipes: An option for “alperujo” use . Revista Iberoamericana de Micologia, 33(4), 242-247.
http://dx.doi.org/10.1016/j.riam.2015.12.001
---------- CHICAGO ----------
Rugolo, M., Levin, L., Lechner, B.E. "Flammulina velutipes: An option for “alperujo” use " . Revista Iberoamericana de Micologia 33, no. 4 (2016) : 242-247.
http://dx.doi.org/10.1016/j.riam.2015.12.001
---------- MLA ----------
Rugolo, M., Levin, L., Lechner, B.E. "Flammulina velutipes: An option for “alperujo” use " . Revista Iberoamericana de Micologia, vol. 33, no. 4, 2016, pp. 242-247.
http://dx.doi.org/10.1016/j.riam.2015.12.001
---------- VANCOUVER ----------
Rugolo, M., Levin, L., Lechner, B.E. Flammulina velutipes: An option for “alperujo” use . Rev. Iberoam. Micol. 2016;33(4):242-247.
http://dx.doi.org/10.1016/j.riam.2015.12.001