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Abstract:

Background: Colletotrichum truncatum is the most common pathogenic fungus associated with soybean anthracnose, a prevalent disease in Argentina. Pectinolytic enzymes are involved in the pathogenicity of a wide range of plant pathogenic fungi. Objectives: To explore pectinolytic enzyme production in Argentinian Colletotrichum strains isolated from diseased soybean plants from different geographic locations, as a preliminary step to establish the biological role of the pectinolytic enzymes in the Colletotrichum spp.-soybean system, yet unknown. Methods: Ten strains were screened for in vitro pectinolytic enzyme production on a defined medium based on pectin as carbon source. Results: All isolates were able to grow in this medium and polymethylgalacturonase (PMG), polygalacturonase (PG) and pectin lyase (PL) activities were detected. On the whole, the peak of polygalacturonases activities preceded the day of maximum growth, while PL activity reached its highest level afterwards. Strain BAFC 3097 (from Santa Fe province) yielded high titles of the three enzymes (1.08. U/ml PG, 1.05. U/ml PMG, 156. U/ml PL), after a short incubation period (7-10 days). Low synthesis of polygalacturonases in cultures containing glucose as unique carbon source suggests that these enzymes are constitutive in contrast with PL, which was not detected. Conclusions: The disparity observed in enzyme production among strains cannot be related to fungal growth, since no major differences in mycelial yield were found; it was not connected with their geographic origin, but might be associated with differences in virulence among strains not yet evaluated. © 2010 Revista Iberoamericana de Micología.

Registro:

Documento: Artículo
Título:Pectinolytic enzyme production by Colletotrichum truncatum, causal agent of soybean anthracnose
Autor:Ramos, A.M.; Gally, M.; García, M.C.; Levin, L.
Filiación:Dpto. de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, PROPLAME-PRHIDEB-CONICET, Universidad de Buenos Aires, Argentina
Cátedra de Fitopatología, Facultad de Agronomía, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina
Palabras clave:Anthracnose; Colletotrichum truncatum; Pectinolytic enzymes; Soybean; pectin; pectin lyase; polygalacturonase; anthracnose; article; Colletotrichum; Colletotrichum truncatum; controlled study; culture medium; enzyme activity; enzyme synthesis; fungal strain; fungal virulence; fungus culture; fungus growth; fungus isolation; geographic distribution; in vitro study; nonhuman; pH measurement; plant pathogen interaction; soybean; Colletotrichum; Pectins; Plant Diseases; Soybeans; Colletotrichum; Colletotrichum truncatum; Fungi; Glycine max
Año:2010
Volumen:27
Número:4
Página de inicio:186
Página de fin:190
DOI: http://dx.doi.org/10.1016/j.riam.2010.06.002
Título revista:Revista Iberoamericana de Micologia
Título revista abreviado:Rev. Iberoam. Micol.
ISSN:11301406
CODEN:RIBME
CAS:pectin, 9000-69-5; pectin lyase, 9033-35-6; polygalacturonase, 9023-92-1, 9032-75-1; Pectins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_11301406_v27_n4_p186_Ramos

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Citas:

---------- APA ----------
Ramos, A.M., Gally, M., García, M.C. & Levin, L. (2010) . Pectinolytic enzyme production by Colletotrichum truncatum, causal agent of soybean anthracnose . Revista Iberoamericana de Micologia, 27(4), 186-190.
http://dx.doi.org/10.1016/j.riam.2010.06.002
---------- CHICAGO ----------
Ramos, A.M., Gally, M., García, M.C., Levin, L. "Pectinolytic enzyme production by Colletotrichum truncatum, causal agent of soybean anthracnose " . Revista Iberoamericana de Micologia 27, no. 4 (2010) : 186-190.
http://dx.doi.org/10.1016/j.riam.2010.06.002
---------- MLA ----------
Ramos, A.M., Gally, M., García, M.C., Levin, L. "Pectinolytic enzyme production by Colletotrichum truncatum, causal agent of soybean anthracnose " . Revista Iberoamericana de Micologia, vol. 27, no. 4, 2010, pp. 186-190.
http://dx.doi.org/10.1016/j.riam.2010.06.002
---------- VANCOUVER ----------
Ramos, A.M., Gally, M., García, M.C., Levin, L. Pectinolytic enzyme production by Colletotrichum truncatum, causal agent of soybean anthracnose . Rev. Iberoam. Micol. 2010;27(4):186-190.
http://dx.doi.org/10.1016/j.riam.2010.06.002