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Abstract:

The ability to degrade organic solid wastes by the fungus Saccobolus saccoboloides was studied. The organism, unusual in such studies, was cultivated in synthetic liquid media with agitation, and on day 8 of growth the mycelium was passed to flasks with trimming. On day 16 of growth, the trimming degradation was assesed by carboximethylcelulase, xylanase, and amylase activities evaluation, and NaOH 1% hydrolysis. Later on, the type of waste was modified (trimming, filter paper, newspaper, cardboard, sawdust and wood shaving were used) as well as the mass (300-1800 mg/flask). In these cases the enzymatic activities increased between 300 and 600 mg/flask. The total separation of the cellular components in all types of paper and cardboard was observed, together with a high loss of weight. S. saccoboloides was not able to degrade the wood wastes.

Registro:

Documento: Artículo
Título:Degradation of lignocellulosic wastes by Saccobolus saccoboloides (fungi, ascomycotina)
Autor:Diorio, L.A.; Forchiassin, F.; Papinutti, V.L.; Sueldo, D.V.
Filiación:Depto. de Biodiversidad y Biol. Exp., Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Depto. de Biodiversidad y Biol. Exp., Fac. de Ciencias Exactas y Naturales, Pabellón II, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Palabras clave:Amylases; Carboximethylcelulase; Saccobolus saccoboloides; Solid wastes degradation; Xylanases; amylase; carboxymethylcellulose; lignocellulose; xylan endo 1,3 beta xylosidase; agitation; article; cell type; enzyme activity; evaluation; filter; fungus; fungus culture; hydrolysis; mycelium; nonhuman; protein degradation; publication; saccobolus saccoboloides; sawdust; synthesis; waste; wood; Amylases; Ascomycota; Biodegradation, Environmental; Cellulase; Cellulose; Culture Media; Fungal Proteins; Glycoside Hydrolases; Industrial Microbiology; Industrial Waste; Lignin; Paper; Refuse Disposal; Wood; Xylan Endo-1,3-beta-Xylosidase; Xylosidases; Ascomycota; Fungi; Saccobolus; Saccobolus saccoboloides
Año:2003
Volumen:20
Número:1
Página de inicio:11
Página de fin:15
Título revista:Revista Iberoamericana de Micologia
Título revista abreviado:Rev. Iberoam. Micol.
ISSN:11301406
CODEN:RIBME
CAS:amylase, 9000-90-2, 9000-92-4, 9001-19-8; carboxymethylcellulose, 8050-38-2, 9000-11-7, 9004-32-4, 9050-04-8; lignocellulose, 11132-73-3; xylan endo 1,3 beta xylosidase, 37278-89-0, 9025-55-2; Amylases, EC 3.2.1.-; carboxymethylcellulase, EC 3.2.1.4; Cellulase, EC 3.2.1.4; Cellulose, 9004-34-6; Culture Media; Fungal Proteins; Glycoside Hydrolases, EC 3.2.1.-; Industrial Waste; Lignin, 9005-53-2; Xylan Endo-1,3-beta-Xylosidase, EC 3.2.1.32; Xylosidases, EC 3.2.1.-
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_11301406_v20_n1_p11_Diorio

Referencias:

  • Medeiros, R.G., Soffner, M.L.A.P., Thome, J.A., The production of hemicellulases by aerobic fungi on medium containing residues of banana plant as substrate (2000) Biotechnol Prog, 16, pp. 522-324
  • Bakir, U., Yavascaoglu, S., Guvenc, F., Ersayin, A., An endo-β-1,4-xylanase from Rhizopus oryzae: Production, partial purification and biochemical characterization (2001) Enzyme Microbiol Technol, 29, pp. 328-334
  • Awafo, V.S., Chahal, D.S., Simpson, B.K., Le, G.B.B., Production of cellulase systems by selected mutants of Trichoderma reesei in solid-state fermentation and their hydrolytic potentials (1996) Appl Biochem Biotechnol, 57, pp. 461-470
  • Atkinson, C.F., Jones, D.D., Gauthier, J.J., Microbial activities during composting of pulp and paper-mill primary solids (1997) W J Microbiol Biotechnol, 13, pp. 519-525
  • Wu, J., Ju, L.K., Enhancing enzymatic saccharification of waste newsprint by surfactant addition (1998) Biotechnol Prog, 14, pp. 649-652
  • Magnelli, P.E., Ramos, A.M., Forchiassin, F., Factors influencing cellulase production by Saccobolus saccoboloides (1996) Mycologia, 88, pp. 249-255
  • Magnelli, P.E., Forchiassin, F., Regulation of the cellulase complex by Saccobolus saccoboloides: Induction and repression by carbohydrates (1999) Mycologia, 91, pp. 359-364
  • Sivori, A.S., Mercuri, O.A., Forchiassin, F., Producción de celulasas por tres especies de Ascobolus (Ascomycotina, Pezizales) (1997) Bol Soc Argent Bot, 33, pp. 1-6
  • Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding (1976) Anal Biochem, 72, pp. 248-254
  • Magnelli, P.E., Martinez, A., Mercuri, O.A., Método simple para determinar actividad celulolítica en hongos (1997) Rev Arg Microbiol, 29, pp. 210-214
  • Procter, A.R., Chow, W.M., A chip quality index for rot (1973) Pulp Paper Mag Can, 74, p. 97
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Citas:

---------- APA ----------
Diorio, L.A., Forchiassin, F., Papinutti, V.L. & Sueldo, D.V. (2003) . Degradation of lignocellulosic wastes by Saccobolus saccoboloides (fungi, ascomycotina) . Revista Iberoamericana de Micologia, 20(1), 11-15.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_11301406_v20_n1_p11_Diorio [ ]
---------- CHICAGO ----------
Diorio, L.A., Forchiassin, F., Papinutti, V.L., Sueldo, D.V. "Degradation of lignocellulosic wastes by Saccobolus saccoboloides (fungi, ascomycotina) " . Revista Iberoamericana de Micologia 20, no. 1 (2003) : 11-15.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_11301406_v20_n1_p11_Diorio [ ]
---------- MLA ----------
Diorio, L.A., Forchiassin, F., Papinutti, V.L., Sueldo, D.V. "Degradation of lignocellulosic wastes by Saccobolus saccoboloides (fungi, ascomycotina) " . Revista Iberoamericana de Micologia, vol. 20, no. 1, 2003, pp. 11-15.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_11301406_v20_n1_p11_Diorio [ ]
---------- VANCOUVER ----------
Diorio, L.A., Forchiassin, F., Papinutti, V.L., Sueldo, D.V. Degradation of lignocellulosic wastes by Saccobolus saccoboloides (fungi, ascomycotina) . Rev. Iberoam. Micol. 2003;20(1):11-15.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_11301406_v20_n1_p11_Diorio [ ]