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Abstract:

During the resolution of inflammation macrophages undergo functional changes upon exposure to pro-resolving agents in their microenvironment. Primarily, engulfment of apoptotic polymorphonuclear (PMN) cells promotes conversion of macrophages toward a pro-resolving phenotype characterized by reduced CD11b expression. These macrophages are not phagocytic, do not respond to TLR ligands, and express relatively high levels of the pro-resolving enzyme 12/15-lipoxygenase (LO). Here, we report that the immuno-regulatory lectin galectin-1 is selectively expressed by CD11bhigh, but not CD11b low macrophages. Upon exposure in vivo and ex vivo, galectin-1 directly promoted macrophage conversion from a CD11bhigh to a CD11blow phenotype and up-regulated the expression and activity of 12/15-LO. Moreover, galectin-1 treatment in vivo promoted the loss of phagocytic capacity (efferocytic satiation) in peritoneal macrophages and down-regulated secretion of TNF-α, IL-1β, and IL-10 upon LPS exposure. Our results suggest that galectin-1 could be an essential mediator in the control of macrophage function during the resolution of inflammation. © 2013 Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype
Autor:Rostoker, R.; Yaseen, H.; Schif-Zuck, S.; Lichtenstein, R.G.; Rabinovich, G.A.; Ariel, A.
Filiación:Department of Biology, Faculty of Natural Sciences, University of Haifa, Haifa 31905, Israel
Department of Biotechnology Engineering, Ben-Gurion University, Be'er Sheva, Israel
Laboratorio de Inmunopatología, Instituto de Biología y Medicina Experimental (IBYME), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), C1428ADN Buenos Aires, Argentina
Laboratorio de Glicómica Estructural y Funcional, IQUIBICEN-CONICET, Departamento de Química Biológica, C1428 Ciudad de Buenos Aires, Argentina
Palabras clave:15-Lipoxygenase; Efferocytosis; Galectin-1; Macrophages; Resolution of inflammation; arachidonate 12 lipoxygenase; arachidonate 15 lipoxygenase; CD11b antigen; galectin 1; interleukin 10; interleukin 1beta; lipopolysaccharide; tumor necrosis factor alpha; animal cell; animal experiment; animal model; article; controlled study; cytokine release; down regulation; enzyme activity; ex vivo study; immunoregulation; in vivo study; macrophage; male; mouse; nonhuman; peritoneum macrophage; phagocytosis; phenotype; protein expression; upregulation; 15-Lipoxygenase; Efferocytosis; Galectin-1; Macrophages; Resolution of inflammation; Animals; Antigens, CD11; Apoptosis; Arachidonate 12-Lipoxygenase; Arachidonate 15-Lipoxygenase; Cells, Cultured; Cytokines; Enzyme Induction; Galectin 1; Inflammation; Lipopolysaccharides; Macrophages, Peritoneal; Male; Mice; Mice, Inbred C57BL; Phenotype
Año:2013
Volumen:107
Página de inicio:85
Página de fin:94
DOI: http://dx.doi.org/10.1016/j.prostaglandins.2013.08.001
Título revista:Prostaglandins and Other Lipid Mediators
Título revista abreviado:Prostaglandins Other Lipid Mediators
ISSN:10988823
CODEN:POLMF
CAS:arachidonate 12 lipoxygenase, 82391-43-3; arachidonate 15 lipoxygenase, 82249-77-2; galectin 1, 258495-34-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10988823_v107_n_p85_Rostoker

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Citas:

---------- APA ----------
Rostoker, R., Yaseen, H., Schif-Zuck, S., Lichtenstein, R.G., Rabinovich, G.A. & Ariel, A. (2013) . Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype. Prostaglandins and Other Lipid Mediators, 107, 85-94.
http://dx.doi.org/10.1016/j.prostaglandins.2013.08.001
---------- CHICAGO ----------
Rostoker, R., Yaseen, H., Schif-Zuck, S., Lichtenstein, R.G., Rabinovich, G.A., Ariel, A. "Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype" . Prostaglandins and Other Lipid Mediators 107 (2013) : 85-94.
http://dx.doi.org/10.1016/j.prostaglandins.2013.08.001
---------- MLA ----------
Rostoker, R., Yaseen, H., Schif-Zuck, S., Lichtenstein, R.G., Rabinovich, G.A., Ariel, A. "Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype" . Prostaglandins and Other Lipid Mediators, vol. 107, 2013, pp. 85-94.
http://dx.doi.org/10.1016/j.prostaglandins.2013.08.001
---------- VANCOUVER ----------
Rostoker, R., Yaseen, H., Schif-Zuck, S., Lichtenstein, R.G., Rabinovich, G.A., Ariel, A. Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype. Prostaglandins Other Lipid Mediators. 2013;107:85-94.
http://dx.doi.org/10.1016/j.prostaglandins.2013.08.001