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Abstract:

Alternative mRNA splicing of the fibronectin EDI exon is controlled by a purine-rich exonic splicing enhancer (ESE), postulated as a binding site for SR proteins. By using a transient expression alternative splicing assay combined with promoter swapping, we have demonstrated that the promoter can also control EDI splicing, arguing for coupling between the transcription and splicing machineries. We now report that the SR proteins SF2/ASF and 9G8 stimulate EDI splicing in vivo and that their effect requires an intact EDI ESE. Most importantly, we show that sensitivity to these SR proteins critically depends on the promoter structure, suggesting that the transcription machinery modulates their recruitment to the ESE.

Registro:

Documento: Artículo
Título:Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer
Autor:Cramer, P.; Cáceres, J.F.; Cazalla, D.; Kadener, S.; Muro, A.F.; Baralle, F.E.; Kornblihtt, A.R.
Filiación:Lab. de Fisiologia y Biologia Molec., Depto. de Ciencias Biológicas, Universidad de Buenos Aires, Buenos Aires (1428), Argentina
Med. Res. Cncl. Human Genetics Unit, Western General Hospital, Edinburgh EH4 2XU, United Kingdom
Intl. Ctr. for Genet. Eng./Biotech., Trieste I-34012, Italy
Palabras clave:alpha globin; DNA; fibronectin; messenger RNA; Pol protein; protein; RNA; transcription factor; article; binding site; cell line; enhancer region; exon; genetic linkage; human; molecular interaction; RNA splicing; transcription regulation
Año:1999
Volumen:4
Número:2
Página de inicio:251
Página de fin:258
DOI: http://dx.doi.org/10.1016/S1097-2765(00)80372-X
Título revista:Molecular Cell
Título revista abreviado:Mol. Cell
ISSN:10972765
CODEN:MOCEF
CAS:DNA, 9007-49-2; fibronectin, 86088-83-7; protein, 67254-75-5; RNA, 63231-63-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10972765_v4_n2_p251_Cramer

Referencias:

  • Alonso, C.A., Pesce, C.G., Kornblihtt, A.R., The CCAAT-binding proteins CP1 and NF-I cooperate with ATF-2 in the transcription of the fibronectin gene (1996) J. Biol. Chem., 271, pp. 22271-22279
  • Cáceres, J.F., Stamm, S., Helfman, D.M., Krainer, A.R., Regulation of alternative splicing in vivo by overexpression of antagonistic splicing factors (1994) Science, 265, pp. 1706-1709
  • Cáceres, J.F., Misteli, T., Screaton, G.R., Spector, D.L., Krainer, A.R., Role of the modular domains of SR proteins in subnuclear localization and alternative splicing specificity (1997) J. Cell Biol., 138, pp. 225-238
  • Cáceres, J.F., Screaton, G.R., Krainer, A.R., A specific subset of SR proteins shuttles continuously between the nucleus and the cytoplasm (1998) Genes Dev., 12, pp. 55-66
  • Cao, W., Jamison, S.F., Garcia-Blanco, M.A., Both phosphorylation and dephosphorylation of ASF/SF2 are required for pre-mRNA splicing in vitro (1997) RNA, 3, pp. 1456-1467
  • Caputi, M., Casari, G., Guenzi, S., Tagliabue, R., Sidoli, A., Melo, C.A., Baralle, F.E., A novel bipartite splicing enhancer modulates the differential processing of the human fibronectin EDA exon (1994) Nucleic Acids Res., 22, pp. 1018-1022
  • Cavaloc, Y., Bourgeois, C.F., Kister, L., Stévenin, J., The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers (1999) RNA, 5, pp. 468-483
  • Chomczynski, P., Sacchi, N., Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction (1987) Annal. Biochem., 162, pp. 156-159
  • Corden, J.L., Patturajan, M., A CTD function linking transcription to splicing (1997) Trends Biochem. Sci., 22, pp. 413-416
  • Cramer, P., Pesce, C.G., Baralle, F.E., Kornblihtt, A.R., Functional association between promoter structure and transcript alternative splicing (1997) Proc. Natl. Acad. Sci. USA, 94, pp. 11456-11460
  • Fu, X.-D., Specific commitment of different pre-mRNAs to splicing by single SR proteins (1993) Nature, 365, pp. 82-85
  • Ge, H., Manley, J.L., A protein factor, ASF, controls cell-specific alternative splicing of SV40 early pre-mRNA in vitro (1990) Cell, 62, pp. 25-34
  • Ge, H., Zuo, P., Manley, J.L., Primary structure of the human splicing factor ASF reveals similarities with Drosophila regulators (1991) Cell, 66, pp. 373-382
  • Ge, H., Si, Y., Wolffe, A.P., A novel transcriptional coactivator, p52, funcionally interacts with the essential splicing factor ASF/SF2 (1998) Mol. Cell, 2, pp. 751-759
  • Hertel, K.J., Lynch, K.W., Maniatis, T., Common themes in the function of transcription and splicing enhancers (1997) Curr. Opin. Cell Biol., 9, pp. 3751-3757
  • Hirose, Y., Manley, J.L., RNA polymerase II is an essential mRNA polyadenylation factor (1998) Nature, 395, pp. 93-96
  • Jiménez-García, L.F., Spector, D.L., In vivo evidence that transcription and splicing are coordinated by a recruiting mechanism (1993) Cell, 73, pp. 47-59
  • Kornblihtt, A.R., Vibe-Pedersen, K., Baralle, F.E., Human fibronectin: Molecular cloning evidence for two mRNA species differing by an internal segment coding for a structural domain (1984) EMBO J., 3, pp. 221-226
  • Kornblihtt, A.R., Pesce, C.G., Alonso, C.R., Cramer, P., Srebrow, A., Werbajh, S.E., Muro, A.F., The fibronectin gene as a model for splicing and transcription studies (1996) FASEB J., 10, pp. 248-257
  • Krainer, A.R., Conway, G.C., Kozak, D., Purification and characterization of pre-mRNA splicing factor SF2 from Hela cells (1990) Genes Dev., 4, pp. 1158-1171
  • Krainer, A.R., Mayeda, A., Kozak, D., Binns, G., Functional expression of cloned human splicing factor SF2: Homology to RNA-binding proteins U1 70k, and Drosophila splicing regulators (1991) Cell, 66, pp. 383-394
  • Lavigueur, A., La Branche, H., Kornblihtt, A.R., Chabot, B., A splicing enhancer in the human fibronectin alternate exon EDI interacts with SR proteins and stimulates U2 snRNP binding (1993) Genes Dev., 7, pp. 2405-2417
  • Manley, J.L., Tacke, R., SR proteins and splicing control (1996) Genes Dev., 10, pp. 1569-1579
  • Mardon, H.J., Sebastio, G., Baralle, F.E., A role for exon sequences in alternative splicing of the human fibronectin gene (1987) Nucleic Acids Res., 15, pp. 7725-7733
  • McCracken, S., Fong, N., Yankulov, K., Ballantyne, S., Pan, G., Greenblatt, J., Patterson, S.D., Bentley, D.L., The C-terminal domain of RNA polymerase II couples mRNA processing to transcription (1997) Nature, 385, pp. 357-361
  • Misteli, T., Spector, D.L., The cellular organization of gene expression (1998) Curr. Opin. Cell Biol., 10, pp. 323-331
  • Misteli, T., Cáceres, J.F., Spector, D.L., The dynamics of a pre-mRNA splicing factor in living cells (1997) Nature, 387, pp. 523-527
  • Muro, A.F., Caputi, M., Pariyarath, R., Pagani, F., Buratti, E., Baralle, F.E., Regulation of fibronectin EDA exon alternative splicing: Possible role of RNA secondary structure for enhancer display (1999) Mol. Cell. Biol., 19, pp. 2657-2671
  • Moore, M.J., Query, C.C., Sharp, P.A., Splicing of precursors to messenger RNAs by the spliceosome (1993) The RNA World, pp. 303-358. , R.F. Gesteland and J.F. Atkins, eds. (Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press)
  • Mortillaro, M.J., Blencowe, B.J., Wei, X., Nakayasu, H., Du, L., Warren, S.L., Sharp, P.A., Berezney, R., A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix (1996) Proc. Natl. Acad. Sci. USA, 93, pp. 8253-8257
  • Nayler, O., Cap, C., Stamm, S., Human transformer-2-beta gene (SFRS10): Complete nucleotide sequence, chromosomal localization, and generation of a tissue-specific isoform (1998) Genomics, 53, pp. 191-202
  • Nayler, O., Strätling, W., Bourquin, J.-P., Stagljar, I., Lindemann, L., Jasper, H., Hartmann, A.M., Stamm, S., SAF-B protein couples transcription and pre-mRNA splicing to SAR/MAR elements (1998) Nucleic Acids Res., 26, pp. 3542-3549
  • O'Brien, T., Hardin, S., Greenleaf, A., Lis, J.T., Phosphorylation of RNA polymerase II C-terminal domain and transcriptional elongation (1994) Nature, 370, pp. 75-77
  • Sun, Q., Mayeda, A., Hampson, R.K., Krainer, A.R., Rottman, F.M., General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer (1993) Genes Dev., 7, pp. 2598-2608
  • Tacke, R., Manley, J.L., The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities (1995) EMBO J., 14, pp. 3540-3551
  • Tacke, R., Tohyama, M., Ogawa, S., Manley, J.L., Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing (1998) Cell, 93, pp. 139-148
  • Vibe-Pedersen, K., Kornblihtt, A.R., Baralle, F.E., Expression of a human α-globin/fibronectin gene hybrid generates two mRNAs by alternative splicing (1984) EMBO J., 3, pp. 2511-2516
  • Wang, J., Takagaki, Y., Manley, J.L., Targeted disruption of an essential vertebrate gene: ASF/SF2 is required for cell viability (1996) Genes Dev., 10, pp. 2588-2599
  • Wang, J., Xiao, S.-H., Manley, J.L., Genetic analysis of the SR protein ASF/SF2: Interchangeability of RS domains and negative control of splicing (1998) Genes Dev., 12, pp. 2222-2233
  • Xiao, S.-H., Manley, J.L., Phosphorylation of the ASF/SF2 RS domain affects both protein-protein and protein-RNA interactions and is necessary for splicing (1997) Genes Dev., 11, pp. 334-344
  • Xing, Y., Johnson, C.V., Dobner, P.R., Bentley Lawrence, J., Higher level organization of individual gene transcription and RNA splicing (1993) Science, 259, pp. 1326-1329
  • Yankulov, K., Blau, J., Purton, T., Roberts, S., Bentley, D.L., Transcriptional elongation by RNA polymerase II is stimulated by transactivators (1994) Cell, 77, pp. 749-759
  • Yuryev, A., Patturajan, M., Litingtung, Y., Joshi, R.V., Gentile, C., Gebara, M., Corden, J.L., The C-terminal domain of the largest subunit of RNA polymerase II interacts with a novel set of serine/arginine-rich proteins (1996) Proc. Natl. Acad. Sci. USA, 93, pp. 6975-6980
  • Zahler, A.M., Neugebauer, K.M., Lane, W.S., Roth, M.B., SR proteins: A conserved family of pre-mRNA splicing factors (1992) Genes Dev., 6, pp. 837-847

Citas:

---------- APA ----------
Cramer, P., Cáceres, J.F., Cazalla, D., Kadener, S., Muro, A.F., Baralle, F.E. & Kornblihtt, A.R. (1999) . Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer. Molecular Cell, 4(2), 251-258.
http://dx.doi.org/10.1016/S1097-2765(00)80372-X
---------- CHICAGO ----------
Cramer, P., Cáceres, J.F., Cazalla, D., Kadener, S., Muro, A.F., Baralle, F.E., et al. "Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer" . Molecular Cell 4, no. 2 (1999) : 251-258.
http://dx.doi.org/10.1016/S1097-2765(00)80372-X
---------- MLA ----------
Cramer, P., Cáceres, J.F., Cazalla, D., Kadener, S., Muro, A.F., Baralle, F.E., et al. "Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer" . Molecular Cell, vol. 4, no. 2, 1999, pp. 251-258.
http://dx.doi.org/10.1016/S1097-2765(00)80372-X
---------- VANCOUVER ----------
Cramer, P., Cáceres, J.F., Cazalla, D., Kadener, S., Muro, A.F., Baralle, F.E., et al. Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer. Mol. Cell. 1999;4(2):251-258.
http://dx.doi.org/10.1016/S1097-2765(00)80372-X