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Abstract:

In addition to their role in DNA repair, recombination events are associated with processes aimed at providing the genetic variability needed for adaptation and evolution of a population. In bacteria, recombination is involved in the appearance of new variants by allowing the incorporation of exogenous DNA or the reshuffling of endogenous sequences. Here we show that HpMutS2, a protein belonging to the MutS2 family in Helicobacter pylori, is not involved in mismatch repair but inhibits homologous and homeologous recombination. Disruption of HpmutS2 leads to an increased efficiency of exogenous DNA incorporation. HpMutS2 has a selective affinity for DNA structures mimicking recombination intermediates with no specificity for homoduplex DNA or mismatches. The purified protein has an ATPase activity stimulated by the same DNA structures. Finally, we show that HpMutS2 inhibits DNA strand exchange reactions in vitro. Thus, MutS2 proteins are candidates for controlling recombination and therefore genetic diversity in bacteria.

Registro:

Documento: Artículo
Título:Suppression of homologous and homeologous recombination by the bacterial MutS2 protein
Autor:Pinto, A.V.; Mathieu, A.; Marsin, S.; Veaute, X.; Ielpi, L.; Labigne, A.; Radicella, J.P.
Filiación:Dept. Radiobiologie Radiopathologie, UMR217 CNRS/CEA, Commsrt. l'Ener. Atom., BP 6, F., Fontenay aux Roses, France
Fundación Instituto Leloir, Fac. de Ciencias Exactas Y Naturales, Univ. de Buenos Aires and CONICET, Buenos Aires, Argentina
U. Pathogenie Bacterienne Muqueuses, Institut Pasteur, F-75724, Paris, France
Palabras clave:bacterial protein; DNA; protein MSH2; article; controlled study; DNA repair; DNA structure; enzyme activity; genetic variability; Helicobacter pylori; mismatch repair; nonhuman; protein purification; sequence homology; Adenosine Triphosphatases; Bacterial Proteins; Base Pair Mismatch; Binding Sites; DNA Repair; DNA, Bacterial; DNA-Binding Proteins; Helicobacter pylori; MutS DNA Mismatch-Binding Protein; Recombination, Genetic; Bacteria (microorganisms); Helicobacter; Helicobacter pylori
Año:2005
Volumen:17
Número:1
Página de inicio:113
Página de fin:120
DOI: http://dx.doi.org/10.1016/j.molcel.2004.11.035
Título revista:Molecular Cell
Título revista abreviado:Mol. Cell
ISSN:10972765
CODEN:MOCEF
CAS:DNA, 9007-49-2; protein MSH2, 153700-72-2; Adenosine Triphosphatases, EC 3.6.1.-; Bacterial Proteins; DNA, Bacterial; DNA-Binding Proteins; MutS DNA Mismatch-Binding Protein, EC 3.6.1.3
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_10972765_v17_n1_p113_Pinto.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10972765_v17_n1_p113_Pinto

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Citas:

---------- APA ----------
Pinto, A.V., Mathieu, A., Marsin, S., Veaute, X., Ielpi, L., Labigne, A. & Radicella, J.P. (2005) . Suppression of homologous and homeologous recombination by the bacterial MutS2 protein. Molecular Cell, 17(1), 113-120.
http://dx.doi.org/10.1016/j.molcel.2004.11.035
---------- CHICAGO ----------
Pinto, A.V., Mathieu, A., Marsin, S., Veaute, X., Ielpi, L., Labigne, A., et al. "Suppression of homologous and homeologous recombination by the bacterial MutS2 protein" . Molecular Cell 17, no. 1 (2005) : 113-120.
http://dx.doi.org/10.1016/j.molcel.2004.11.035
---------- MLA ----------
Pinto, A.V., Mathieu, A., Marsin, S., Veaute, X., Ielpi, L., Labigne, A., et al. "Suppression of homologous and homeologous recombination by the bacterial MutS2 protein" . Molecular Cell, vol. 17, no. 1, 2005, pp. 113-120.
http://dx.doi.org/10.1016/j.molcel.2004.11.035
---------- VANCOUVER ----------
Pinto, A.V., Mathieu, A., Marsin, S., Veaute, X., Ielpi, L., Labigne, A., et al. Suppression of homologous and homeologous recombination by the bacterial MutS2 protein. Mol. Cell. 2005;17(1):113-120.
http://dx.doi.org/10.1016/j.molcel.2004.11.035