Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

RhoA regulates the actin cytoskeleton and the expression of genes associated with cell proliferation. This includes c-fos and c-jun, which are members of the AP1 family of transcription factors that play a key role in normal and aberrant cell growth. Whereas RhoA stimulates the c-fos SRE by a recently elucidated mechanism that is dependent on actin treadmilling, how RhoA regulates c-jun is still poorly understood. We found that RhoA stimulates c-jun expression through ROCK, but independently from the ability of ROCK to promote actin polymerization. Instead, we found that ROCK activates JNK, which then phosphorylates c-Jun and ATF2 when bound to the c-jun promoter. Thus, ROCK represents a point of signal divergence downstream from RhoA, as it promotes actin reorganization and the consequent expression from the c-fos SRE, while a parallel pathway connects ROCK to JNK, thereby stimulating c-jun expression. Ultimately, these pathways converge in the nucleus to regulate AP1 activity.

Registro:

Documento: Artículo
Título:The small GTP-binding protein RhoA regulates c-jun by a ROCK-JNK signaling axis
Autor:Marinissen, M.J.; Chiariello, M.; Tanos, T.; Bernard, O.; Narumiya, S.; Gutkind, J.S.
Filiación:Oral and Pharyngeal Cancer Branch, Natl. Inst. Dent. Craniofacial Res., National Institutes of Health, Bethesda, MD 20892, United States
Facultad de Ciencias Exactas, Universidad de Buenos Aires, Buenos Aires, Argentina
Ist. Endocrinol. Ed Oncol. Sperim., CNR, Via Pansini 5, 80131 Napoli, Italy
Walter Eliza Hall Inst. of Med. Res., 1G Royal Parade Parkville, Victoria 3050, Australia
Department of Pharmacology, Kyoto University, Faculty of Medicine, Sakyo-ku, Kyoto 606-8501, Japan
Palabras clave:actin; activating transcription factor 2; protein; protein c fos; protein c jun; protein kinase; protein Rock; RhoA guanine nucleotide binding protein; stress activated protein kinase; transcription factor AP 1; unclassified drug; animal cell; article; cell growth; cell nucleus; cell proliferation; controlled study; cytoskeleton; downstream processing; enzyme activation; enzyme activity; gene expression; human; human cell; molecular mechanics; mouse; nonhuman; polymerization; promoter region; protein binding; protein expression; protein family; protein function; protein phosphorylation; regulatory mechanism; serum responsive element; signal transduction; stimulation; Actin Depolymerizing Factors; Actins; Activating Transcription Factor 2; Animals; Cell Line; Cyclic AMP Response Element-Binding Protein; Cytoskeleton; Enzyme Activation; Enzyme Inhibitors; Gene Expression Regulation; Humans; Intracellular Signaling Peptides and Proteins; JNK Mitogen-Activated Protein Kinases; Lysophospholipids; Mice; Microfilament Proteins; Mitogen-Activated Protein Kinases; NIH 3T3 Cells; Promoter Regions (Genetics); Protein Kinases; Protein-Serine-Threonine Kinases; Proto-Oncogene Proteins c-jun; rhoA GTP-Binding Protein; Signal Transduction; Transcription Factors; Animalia
Año:2004
Volumen:14
Número:1
Página de inicio:29
Página de fin:41
DOI: http://dx.doi.org/10.1016/S1097-2765(04)00153-4
Título revista:Molecular Cell
Título revista abreviado:Mol. Cell
ISSN:10972765
CODEN:MOCEF
CAS:protein kinase, 9026-43-1; protein, 67254-75-5; stress activated protein kinase, 155215-87-5; Actin Depolymerizing Factors; Actins; Activating Transcription Factor 2; ATF2 protein, human; Atf2 protein, mouse; Cyclic AMP Response Element-Binding Protein; Enzyme Inhibitors; Intracellular Signaling Peptides and Proteins; JNK Mitogen-Activated Protein Kinases, EC 2.7.1.37; LIM kinase, EC 2.7.1.-; lysophosphatidic acid, 22002-87-5; Lysophospholipids; Microfilament Proteins; Mitogen-Activated Protein Kinases, EC 2.7.1.37; Protein Kinases, EC 2.7.1.37; Protein-Serine-Threonine Kinases, EC 2.7.1.37; Proto-Oncogene Proteins c-jun; Rho-associated kinase, EC 2.7.1.-; rhoA GTP-Binding Protein, EC 3.6.5.2; Transcription Factors
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10972765_v14_n1_p29_Marinissen

Referencias:

  • Alberts, A.S., Geneste, O., Treisman, R., Activation of SRF-regulated chromosomal templates by Rho-family GTPases requires a signal that also induces H4 hyperacetylation (1998) Cell, 92, pp. 475-487
  • Alberts, A.S., Treisman, R., Activation of RhoA and SAPK/JNK signalling pathways by the RhoA-specific exchange factor mNET1 (1998) EMBO J., 17, pp. 4075-4085
  • Arber, S., Barbayannis, F.A., Hanser, H., Schneider, C., Stanyon, C.A., Bernard, O., Caroni, P., Regulation of actin dynamics through phosphorylation of cofilin by LIM-kinase (1998) Nature, 393, pp. 805-809
  • Bar-Sagi, D., Hall, A., Ras and Rho GTPases: A family reunion (2000) Cell, 103, pp. 227-238
  • Brown, P.H., Alani, R., Preis, L.H., Szabo, E., Birrer, M.J., Suppression of oncogene-induced transformation by a deletion mutant of c-jun (1993) Oncogene, 8, pp. 877-886
  • Chiariello, M., Marinissen, M.J., Gutkind, J.S., Multiple mitogen-activated protein kinase signaling pathways connect the cot oncoprotein to the c-jun promoter and to cellular transformation (2000) Mol. Cell. Biol., 20, pp. 1747-1758
  • Clarke, N., Arenzana, N., Hai, T., Minden, A., Prywes, R., Epidermal growth factor induction of the c-jun promoter by a Rac pathway (1998) Mol. Cell. Biol., 18, pp. 1065-1073
  • Coso, O.A., Chiariello, M., Yu, J.C., Teramoto, H., Crespo, P., Xu, N., Miki, T., Gutkind, J.S., The small GTP-binding proteins Rac1 and Cdc42 regulate the activity of the JNK/SAPK signaling pathway (1995) Cell, 81, pp. 1137-1146
  • Davis, R.J., Signal transduction by the JNK group of MAP kinases (2000) Cell, 103, pp. 239-252
  • Fukuhara, S., Murga, C., Zohar, M., Igishi, T., Gutkind, J.S., A novel PDZ domain containing guanine nucleotide exchange factor links heterotrimeric G proteins to Rho (1999) J. Biol. Chem., 274, pp. 5868-5879
  • Geneste, O., Copeland, J.W., Treisman, R., LIM kinase and Diaphanous cooperate to regulate serum response factor and actin dynamics (2002) J. Cell Biol., 157, pp. 831-838
  • Han, T.H., Lamph, W.W., Prywes, R., Mapping of epidermal growth factor-, serum-, and phorbol ester-responsive sequence elements in the c-jun promoter (1992) Mol. Cell. Biol., 12, pp. 4472-4477
  • Herr, I., Van Dam, H., Angel, P., Binding of promoter-associated AP-1 is not altered during induction and subsequent repression of the c-jun promoter by TPA and UV irradiation (1994) Carcinogenesis, 15, pp. 1105-1113
  • Hill, C.S., Wynne, J., Treisman, R., The Rho family GTPases RhoA, Rac1, and CDC42Hs regulate transcriptional activation by SRF (1995) Cell, 81, pp. 1159-1170
  • Iavarone, C., Catania, A., Marinissen, M.J., Visconti, R., Acunzo, M., Tarantino, C., Carlomagno, M.S., Chiariello, M., The platelet-derived growth factor controls c-myc expression through a JNK- and AP-1-dependent signaling pathway (2003) J. Biol. Chem., 278, pp. 50024-50030
  • Itoh, K., Yoshioka, K., Akedo, H., Uehata, M., Ishizaki, T., Narumiya, S., An essential part for Rho-associated kinase in the transcellular invasion of tumor cells (1999) Nat. Med., 5, pp. 221-225
  • Karin, M., Liu, Z., Zandi, E., AP-1 function and regulation (1997) Curr. Opin. Cell Biol., 9, pp. 240-246
  • Marinissen, M.J., Chiariello, M., Gutkind, J.S., Regulation of gene expression by the small GTPase Rho through the ERK6 (p38 gamma) MAP kinase pathway (2001) Genes Dev., 15, pp. 535-553
  • Marinissen, M.J., Chiariello, M., Pallante, M., Gutkind, J.S., A network of mitogen-activated protein kinases links G protein-coupled receptors to the c-jun promoter: A role for c-Jun NH2-terminal kinase, p38s, and extracellular signal-regulated kinase 5 (1999) Mol. Cell. Biol., 19, pp. 4289-4301
  • Minden, A., Lin, A., Claret, F.X., Abo, A., Karin, M., Selective activation of the JNK signaling cascade and c-Jun transcriptional activity by the small GTPases Rac and Cdc42Hs (1995) Cell, 81, pp. 1147-1157
  • Miralles, F., Posern, G., Zaromytidou, A.I., Treisman, R., Actin dynamics control SRF activity by regulation of its coactivator MAL (2003) Cell, 113, pp. 329-342
  • Nagao, M., Kaziro, Y., Itoh, H., The Src family tyrosine kinase is involved in Rho-dependent activation of c-Jun N-terminal kinase by Galpha12 (1999) Oncogene, 18, pp. 4425-4434
  • Narumiya, S., The small GTPase Rho: Cellular functions and signal transduction (1996) J. Biochem. (Tokyo), 120, pp. 215-228
  • Narumiya, S., Ishizaki, T., Uehata, M., Use and properties of ROCK-specific inhibitor Y-27632 (2000) Methods Enzymol., 325, pp. 273-284
  • Noguchi, K., Kitanaka, C., Yamana, H., Kokubu, A., Mochizuki, T., Kuchino, Y., Regulation of c-Myc through phosphorylation at Ser-62 and Ser-71 by c-Jun N-terminal kinase (1999) J. Biol. Chem., 274, pp. 32580-32587
  • Ridley, A.J., Hall, A., The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors (1992) Cell, 70, pp. 389-399
  • Sahai, E., Alberts, A.S., Treisman, R., RhoA effector mutants reveal distinct effector pathways for cytoskeletal reorganization, SRF activation and transformation (1998) EMBO J., 17, pp. 1350-1361
  • Sahai, E., Ishizaki, T., Narumiya, S., Treisman, R., Transformation mediated by RhoA requires activity of ROCK kinases (1999) Curr. Biol., 9, pp. 136-145
  • Sotiropoulos, A., Gineitis, D., Copeland, J., Treisman, R., Signal-regulated activation of serum response factor is mediated by changes in actin dynamics (1999) Cell, 98, pp. 159-169
  • Strutt, D.I., Weber, U., Mlodzik, M., The role of RhoA in tissue polarity and Frizzled signalling (1997) Nature, 387, pp. 292-295
  • Takahashi, M., Gotoh, Y., Isagawa, T., Nishimura, T., Goyama, E., Kim, H.S., Mukai, H., Ono, Y., Regulation of a Mitogen-Activated Protein Kinase Kinase Kinase, MLTK by PKN (2003) J. Biochem. (Tokyo), 133, pp. 181-187
  • Takai, Y., Sasaki, T., Matozaki, T., Small GTP-binding proteins (2001) Physiol. Rev., 81, pp. 153-208
  • Teramoto, H., Crespo, P., Coso, O.A., Igishi, T., Xu, N., Gutkind, J.S., The small GTP-binding protein rho activates c-Jun N-terminal kinases/stress-activated protein kinases in human kidney 293T cells. Evidence for a Pak-independent signaling pathway (1996) J. Biol. Chem., 271, pp. 25731-25734
  • Treisman, R., Journey to the surface of the cell: Fos regulation and the SRE (1995) EMBO J., 14, pp. 4905-4913
  • Watnick, R.S., Cheng, Y.N., Rangarajan, A., Ince, T.A., Weinberg, R.A., Ras modulates Myc activity to repress thrombospondin-1 expression and increase tumor angiogenesis (2003) Cancer Cell, 3, pp. 219-231
  • Whitmarsh, A.J., Shore, P., Sharrocks, A.D., Davis, R.J., Integration of MAP kinase signal transduction pathways at the serum response element (1995) Science, 269, pp. 403-407
  • Whitmarsh, A.J., Yang, S.H., Su, M.S., Sharrocks, A.D., Davis, R.J., Role of p38 and JNK mitogen-activated protein kinases in the activation of ternary complex factors (1997) Mol. Cell. Biol., 17, pp. 2360-2371
  • Zohn, I.M., Campbell, S.L., Khosravi-Far, R., Rossman, K.L., Der, C.J., Rho family proteins and Ras transformation: The RHOad less traveled gets congested (1998) Oncogene, 17, pp. 1415-1438

Citas:

---------- APA ----------
Marinissen, M.J., Chiariello, M., Tanos, T., Bernard, O., Narumiya, S. & Gutkind, J.S. (2004) . The small GTP-binding protein RhoA regulates c-jun by a ROCK-JNK signaling axis. Molecular Cell, 14(1), 29-41.
http://dx.doi.org/10.1016/S1097-2765(04)00153-4
---------- CHICAGO ----------
Marinissen, M.J., Chiariello, M., Tanos, T., Bernard, O., Narumiya, S., Gutkind, J.S. "The small GTP-binding protein RhoA regulates c-jun by a ROCK-JNK signaling axis" . Molecular Cell 14, no. 1 (2004) : 29-41.
http://dx.doi.org/10.1016/S1097-2765(04)00153-4
---------- MLA ----------
Marinissen, M.J., Chiariello, M., Tanos, T., Bernard, O., Narumiya, S., Gutkind, J.S. "The small GTP-binding protein RhoA regulates c-jun by a ROCK-JNK signaling axis" . Molecular Cell, vol. 14, no. 1, 2004, pp. 29-41.
http://dx.doi.org/10.1016/S1097-2765(04)00153-4
---------- VANCOUVER ----------
Marinissen, M.J., Chiariello, M., Tanos, T., Bernard, O., Narumiya, S., Gutkind, J.S. The small GTP-binding protein RhoA regulates c-jun by a ROCK-JNK signaling axis. Mol. Cell. 2004;14(1):29-41.
http://dx.doi.org/10.1016/S1097-2765(04)00153-4