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Abstract:

Changes in promoter structure and occupation have been shown to modify the splicing pattern of several genes, evidencing a coupling between transcription and alternative splicing. It has been proposed that the promoter effect involves modulation of RNA pol II elongation rates. The C4 point mutation of the Drosophila pol II largest subunit confers on the enzyme a lower elongation rate. Here we show that expression of a human equivalent to Drosophila's C4 pol II in human cultured cells affects alternative splicing of the fibronectin EDI exon and adenovirus E1a pre-mRNA. Most importantly, resplicing of the Hox gene Ultrabithorax is stimulated in Drosophila embryos mutant for C4, which demonstrates the transcriptional control of alternative splicing on an endogenous gene. These results provide a direct proof for the elongation control of alternative splicing in vivo.

Registro:

Documento: Artículo
Título:A slow RNA polymerase II affects alternative splicing in vivo
Autor:De La Mata, M.; Alonso, C.R.; Kadener, S.; Fededa, J.P.; Blaustein, M.; Pelisch, F.; Cramer, P.; Bentley, D.; Kornblihtt, A.R.
Filiación:Lab. de Fisiol. y Biol. Molecular, Depto. Fisiol., Biol. Molec. y Cel., Pabellón 2, (C1428EHA) Buenos Aires, Argentina
Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, United Kingdom
Dept. of Biochem./Molecular Genetics, UCSC B121, 4200 East 9th Avenue, Denver, CO 80262, United States
HHMI, Brandeis University, Waltham, MA 02454, United States
Harvard University, Dept. of Molecular and Cell Biology, 7 Divinity Avenue, Cambridge, MA 02138, United States
Palabras clave:elongation factor; fibronectin; protein subunit; RNA; RNA polymerase II; Adenovirus; article; cell culture; Drosophila; embryo cell; exon; gene; genetic transcription; genetic transfection; Hox gene; human; in vivo study; point mutation; protein expression; reverse transcription polymerase chain reaction; RNA splicing; RNA translation; Western blotting; Adenoviridae
Año:2003
Volumen:12
Número:2
Página de inicio:525
Página de fin:532
DOI: http://dx.doi.org/10.1016/j.molcel.2003.08.001
Título revista:Molecular Cell
Título revista abreviado:Mol. Cell
ISSN:10972765
CODEN:MOCEF
CAS:fibronectin, 86088-83-7; RNA, 63231-63-0
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_10972765_v12_n2_p525_DeLaMata.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10972765_v12_n2_p525_DeLaMata

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Citas:

---------- APA ----------
De La Mata, M., Alonso, C.R., Kadener, S., Fededa, J.P., Blaustein, M., Pelisch, F., Cramer, P.,..., Kornblihtt, A.R. (2003) . A slow RNA polymerase II affects alternative splicing in vivo. Molecular Cell, 12(2), 525-532.
http://dx.doi.org/10.1016/j.molcel.2003.08.001
---------- CHICAGO ----------
De La Mata, M., Alonso, C.R., Kadener, S., Fededa, J.P., Blaustein, M., Pelisch, F., et al. "A slow RNA polymerase II affects alternative splicing in vivo" . Molecular Cell 12, no. 2 (2003) : 525-532.
http://dx.doi.org/10.1016/j.molcel.2003.08.001
---------- MLA ----------
De La Mata, M., Alonso, C.R., Kadener, S., Fededa, J.P., Blaustein, M., Pelisch, F., et al. "A slow RNA polymerase II affects alternative splicing in vivo" . Molecular Cell, vol. 12, no. 2, 2003, pp. 525-532.
http://dx.doi.org/10.1016/j.molcel.2003.08.001
---------- VANCOUVER ----------
De La Mata, M., Alonso, C.R., Kadener, S., Fededa, J.P., Blaustein, M., Pelisch, F., et al. A slow RNA polymerase II affects alternative splicing in vivo. Mol. Cell. 2003;12(2):525-532.
http://dx.doi.org/10.1016/j.molcel.2003.08.001