Artículo

Chiappini, F.; Alvarez, L.; Lux-Lantos, V.; Randi, A.S.; de Pisarev, D.L.K. "Hexachlorobenzene triggers apoptosis in rat thyroid follicular cells" (2009) Toxicological Sciences. 108(2):301-310
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Abstract:

Hexachlorobenzene (HCB) is a widespread environmental pollutant. Chronic exposure of humans to HCB produces a number of effects, such as triggering of porphyria, increased synthesis of liver microsomal enzymes, neurological symptoms, immunological disorders and thyroid dysfunctions. In rats, HCB induced hepatic porphyria, neurotoxic effects, and toxic effects on the reproductive system, thyroid function, and immune system. HCB is also known to cause tumors of the liver, thyroid and mammary gland in laboratory animals. The aim of this study was to investigate parameters of thyroid growth regulation, mainly cell proliferation and apoptosis in thyroid tissue from HCB (0.1, 1, 10, 100, and 500 mg/kg body weight)-treated female Wistar rats. The current study demonstrates that only the exposure to the highest HCB dose for 30 days, has adverse effects on thyroid endpoints examined related to thyroid gland morphology, and 3,3′5,5′-tetraiodothyronine (T 4 , thyroxine) serum levels, without changes in thyroid-stimulating hormone concentrations or in thyroid gland weight. Morphological changes, included flattened epithelium and increased colloid size compared with control tissue. Transforming growth factor (TGF-β1) mRNA levels, evaluated by RT-PCR, revealed a significant upregulation after exposure to HCB (1, 10, 100 mg/kg body weight). Cell proliferation evaluated by 5′-Br deoxiuridine incorporation into DNA, was not altered at any dose. HCB (1, 10, 100 mg/kg body weight) induces apoptosis, evaluated by in situ end labeling of fragmented DNA, terminal deoxynucleotidyl transferase-mediated deoxy uridine triphosphate nick-end labeling, in rat thyroid glands. This process is associated with dose-dependent increases in cytochrome c release from the mitochondria and procaspase-9 processing to its active product. Caspase-8 was not activated. These studies indicate that doses of HCB that do not disrupt thyroid economy induce TGF-β1 expression and apoptosis in the thyroid gland, involving the mitochondrial pathway. © The Author 2009. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved.

Registro:

Documento: Artículo
Título:Hexachlorobenzene triggers apoptosis in rat thyroid follicular cells
Autor:Chiappini, F.; Alvarez, L.; Lux-Lantos, V.; Randi, A.S.; de Pisarev, D.L.K.
Filiación:Departamento de Bioquímica Humana, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, Buenos Aires 1121, Argentina
Instituto de Biología y Medicina Experimental, Universidad de Buenos Aires, Buenos Aires 1428, Argentina
Palabras clave:Apoptosis; Hexachlorobenzene; Rat thyroid; caspase 9; cytochrome c; DNA nucleotidylexotransferase; hexachlorobenzene; messenger RNA; procaspase 8; thyroid hormone; thyroxine; transforming growth factor beta1; animal cell; animal experiment; animal tissue; apoptosis; article; cell count; cell proliferation; controlled study; DNA fragmentation; female; gene expression; growth regulation; nick end labeling; nonhuman; protein secretion; rat; regulatory mechanism; reverse transcription polymerase chain reaction; thyroid follicle cell; thyroid hormone blood level; thyroid weight; tissue structure; upregulation; Animals; Apoptosis; Blotting, Western; Caspase 8; Caspase 9; Cell Proliferation; DNA Fragmentation; Endocrine Disruptors; Environmental Pollutants; Enzyme Induction; Female; Hexachlorobenzene; In Situ Nick-End Labeling; Mitochondria; Organ Size; Rats; Rats, Wistar; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Signal Transduction; Subcellular Fractions; Thyroid Gland; Thyroid Hormones; Transforming Growth Factor beta1; Animalia; Rattus; Rattus norvegicus
Año:2009
Volumen:108
Número:2
Página de inicio:301
Página de fin:310
DOI: http://dx.doi.org/10.1093/toxsci/kfp016
Título revista:Toxicological Sciences
Título revista abreviado:Toxicol. Sci.
ISSN:10966080
CODEN:TOSCF
CAS:DNA nucleotidylexotransferase, 9027-67-2; caspase 9, 180189-96-2; cytochrome c, 9007-43-6, 9064-84-0; hexachlorobenzene, 118-74-1, 55600-34-5; thyroxine, 7488-70-2; Caspase 8, 3.4.22.-; Caspase 9, 3.4.22.-; Endocrine Disruptors; Environmental Pollutants; Hexachlorobenzene, 118-74-1; RNA, Messenger; Thyroid Hormones; Transforming Growth Factor beta1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10966080_v108_n2_p301_Chiappini

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Citas:

---------- APA ----------
Chiappini, F., Alvarez, L., Lux-Lantos, V., Randi, A.S. & de Pisarev, D.L.K. (2009) . Hexachlorobenzene triggers apoptosis in rat thyroid follicular cells. Toxicological Sciences, 108(2), 301-310.
http://dx.doi.org/10.1093/toxsci/kfp016
---------- CHICAGO ----------
Chiappini, F., Alvarez, L., Lux-Lantos, V., Randi, A.S., de Pisarev, D.L.K. "Hexachlorobenzene triggers apoptosis in rat thyroid follicular cells" . Toxicological Sciences 108, no. 2 (2009) : 301-310.
http://dx.doi.org/10.1093/toxsci/kfp016
---------- MLA ----------
Chiappini, F., Alvarez, L., Lux-Lantos, V., Randi, A.S., de Pisarev, D.L.K. "Hexachlorobenzene triggers apoptosis in rat thyroid follicular cells" . Toxicological Sciences, vol. 108, no. 2, 2009, pp. 301-310.
http://dx.doi.org/10.1093/toxsci/kfp016
---------- VANCOUVER ----------
Chiappini, F., Alvarez, L., Lux-Lantos, V., Randi, A.S., de Pisarev, D.L.K. Hexachlorobenzene triggers apoptosis in rat thyroid follicular cells. Toxicol. Sci. 2009;108(2):301-310.
http://dx.doi.org/10.1093/toxsci/kfp016