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Chaufan, G.; Corvi, M.; Armesto, A.; San Martín de Viale, L.C.; Luquet, C.; Ríos de Molina, M.C. "Comparison between crab hepatopancreas and rat liver uroporphyrinogen decarboxylase" (2002) Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology. 133(2):251-256
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Abstract:

We characterized Uroporphyrinogen decarboxylase (UroD) (E.C. 4.1.1.37) in hepatopancreas of the crab Chasmagnathus granulatus as a first step to establish this enzyme as a possible biomarker for environmental contamination. We performed a comparative study of crab UroD with the enzyme UroD present in Wistar rat liver, which is known as a useful indicator of intoxication by polyhalogenated aromatic hydrocarbons (PAHs). The final products were the same in crab and rat UroD: the remaining substrate (8-carboxyl-porphyrinogen), the final product Coproporphyrinogen (4-COOH) and intermediate compounds with 7-, 6- and 5-COOH. The elimination of the second carboxyl group seems to be the rate-limiting step in this multiple decarboxylation, because large amounts of 7-COOH porphyrinogen are accumulated. The Vmax/Km ratio was 100-fold higher for rat liver UroD than for crab hepatopancreas UroD, suggesting a higher efficiency of the rat enzyme. Optimum pH for enzyme activity was 7.2 and 6.8 for crab and rat, respectively. Although both systems showed the same optimum temperature (47°C), the activation energy was clearly different, 51.5 kJ/mol for C. granulatus and 5.4 kJ/mol for Rattus norvegicus (Wistar strain). Superdex 75 gel chromatography yielded a single symmetrical peak with an apparent molecular mass of 48±3 kDa for crab hepatopancreas UroD, suggesting the existence of only one enzymatic species in C. granulatus. © 2002 Elsevier Science Inc. All rights reserved.

Registro:

Documento: Artículo
Título:Comparison between crab hepatopancreas and rat liver uroporphyrinogen decarboxylase
Autor:Chaufan, G.; Corvi, M.; Armesto, A.; San Martín de Viale, L.C.; Luquet, C.; Ríos de Molina, M.C.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Activation energy; Crab hepatopancreas; Enzymatic properties; Haem; Kinetic parameters; Optimum pH; Optimum temperature; Porphyrins; Rat liver; Uroporphyrinogen decarboxylase; 8 carboxylporphyrinogen; aromatic hydrocarbon; biological marker; carboxyl group; coproporphyrinogen oxidase; liver enzyme; porphyrinogen; unclassified drug; uroporphyrinogen decarboxylase; animal experiment; animal model; animal tissue; article; chasmagnathus granulatus; controlled study; crab; decarboxylation; enzyme activity; female; gel chromatography; hepatopancreas; intoxication; liver; male; molecular weight; nonhuman; pH; pollutant; priority journal; rat; temperature; Animals; Decapoda (Crustacea); Decarboxylation; Digestive System; Environmental Pollutants; Hydrocarbons, Halogenated; Hydrogen-Ion Concentration; Kinetics; Liver; Porphyrinogens; Rats; Rats, Wistar; Temperature; Uroporphyrinogen Decarboxylase; Chasmagnathus granulata; Decapoda (Crustacea); Rattus; Rattus norvegicus
Año:2002
Volumen:133
Número:2
Página de inicio:251
Página de fin:256
DOI: http://dx.doi.org/10.1016/S1096-4959(02)00151-3
Título revista:Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Título revista abreviado:Comp. Biochem. Physiol. B Biochem. Mol. Biol.
ISSN:10964959
CODEN:CBPBB
CAS:Environmental Pollutants; Hydrocarbons, Halogenated; Porphyrinogens; Uroporphyrinogen Decarboxylase, EC 4.1.1.37
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10964959_v133_n2_p251_Chaufan

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Citas:

---------- APA ----------
Chaufan, G., Corvi, M., Armesto, A., San Martín de Viale, L.C., Luquet, C. & Ríos de Molina, M.C. (2002) . Comparison between crab hepatopancreas and rat liver uroporphyrinogen decarboxylase. Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 133(2), 251-256.
http://dx.doi.org/10.1016/S1096-4959(02)00151-3
---------- CHICAGO ----------
Chaufan, G., Corvi, M., Armesto, A., San Martín de Viale, L.C., Luquet, C., Ríos de Molina, M.C. "Comparison between crab hepatopancreas and rat liver uroporphyrinogen decarboxylase" . Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology 133, no. 2 (2002) : 251-256.
http://dx.doi.org/10.1016/S1096-4959(02)00151-3
---------- MLA ----------
Chaufan, G., Corvi, M., Armesto, A., San Martín de Viale, L.C., Luquet, C., Ríos de Molina, M.C. "Comparison between crab hepatopancreas and rat liver uroporphyrinogen decarboxylase" . Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, vol. 133, no. 2, 2002, pp. 251-256.
http://dx.doi.org/10.1016/S1096-4959(02)00151-3
---------- VANCOUVER ----------
Chaufan, G., Corvi, M., Armesto, A., San Martín de Viale, L.C., Luquet, C., Ríos de Molina, M.C. Comparison between crab hepatopancreas and rat liver uroporphyrinogen decarboxylase. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 2002;133(2):251-256.
http://dx.doi.org/10.1016/S1096-4959(02)00151-3