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Abstract:

Glyphosate-based herbicides (GF) are extensively used for weed control. Thus, it is important to investigate their putative toxic effects. We have reported that GF at subagriculture concentrations inhibits proliferation and differentiation to adipocytes of 3T3-L1 fibroblasts. In this investigation, we evaluated the effect of GF on genes upregulated during adipogenesis. GF was able to inhibit the induction of PPAR gamma, the master gene in adipogenesis but not C/EBP beta, which precedes PPAR gamma activation. GF also inhibited differentiation and proliferation of another model of preadipocyte: mouse embryonic fibroblasts. In exponentially growing 3T3-L1 cells, GF increased lipid peroxidation and the activity of the antioxidant enzyme, superoxide dismutase. We also found that proliferation was inhibited with lower concentrations of GF when time of exposure was extended. Thus, GF was able to inhibit proliferation and differentiation of preadipocytes and to induce oxidative stress, which is indicative of its ability to alter cellular physiology. © 2016 Wiley Periodicals, Inc.

Registro:

Documento: Artículo
Título:Glyphosate Inhibits PPAR Gamma Induction and Differentiation of Preadipocytes and is able to Induce Oxidative Stress
Autor:Martini, C.N.; Gabrielli, M.; Brandani, J.N.; Vila, M.D.C.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, 1428, Buenos Aires, Argentina
Palabras clave:3T3-L1 Fibroblasts; Differentiation; Glyphosate Based-Herbicide; MEFs; Oxidative Stress; CCAAT enhancer binding protein beta; fat droplet; glyphosate; hydrogen peroxide; messenger RNA; peroxisome proliferator activated receptor gamma; superoxide dismutase; 3T3 cell line; adipogenesis; animal cell; Article; cell differentiation; cell proliferation; controlled study; dilution; embryo; enzyme activity; immunofluorescence; lipid peroxidation; lipid storage; mouse; nonhuman; oxidative stress; proadipocyte; reverse transcription polymerase chain reaction; upregulation; Western blotting
Año:2016
Volumen:30
Número:8
Página de inicio:404
Página de fin:413
DOI: http://dx.doi.org/10.1002/jbt.21804
Título revista:Journal of Biochemical and Molecular Toxicology
Título revista abreviado:J. Biochem. Mol. Toxicol.
ISSN:10956670
CODEN:JBMTF
CAS:glyphosate, 1071-83-6; hydrogen peroxide, 7722-84-1; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10956670_v30_n8_p404_Martini

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Citas:

---------- APA ----------
Martini, C.N., Gabrielli, M., Brandani, J.N. & Vila, M.D.C. (2016) . Glyphosate Inhibits PPAR Gamma Induction and Differentiation of Preadipocytes and is able to Induce Oxidative Stress. Journal of Biochemical and Molecular Toxicology, 30(8), 404-413.
http://dx.doi.org/10.1002/jbt.21804
---------- CHICAGO ----------
Martini, C.N., Gabrielli, M., Brandani, J.N., Vila, M.D.C. "Glyphosate Inhibits PPAR Gamma Induction and Differentiation of Preadipocytes and is able to Induce Oxidative Stress" . Journal of Biochemical and Molecular Toxicology 30, no. 8 (2016) : 404-413.
http://dx.doi.org/10.1002/jbt.21804
---------- MLA ----------
Martini, C.N., Gabrielli, M., Brandani, J.N., Vila, M.D.C. "Glyphosate Inhibits PPAR Gamma Induction and Differentiation of Preadipocytes and is able to Induce Oxidative Stress" . Journal of Biochemical and Molecular Toxicology, vol. 30, no. 8, 2016, pp. 404-413.
http://dx.doi.org/10.1002/jbt.21804
---------- VANCOUVER ----------
Martini, C.N., Gabrielli, M., Brandani, J.N., Vila, M.D.C. Glyphosate Inhibits PPAR Gamma Induction and Differentiation of Preadipocytes and is able to Induce Oxidative Stress. J. Biochem. Mol. Toxicol. 2016;30(8):404-413.
http://dx.doi.org/10.1002/jbt.21804