Artículo

Chaufan, G.; Corvi, M.M.; San Martín De Viale, L.C.; Cárdenas, M.L.; Ríos De Molina, M.D.C. "Abnormal kinetic behavior of uroporphyrinogen decarboxylase obtained from rats with hexachlorobenzene-induced porphyria" (2005) Journal of Biochemical and Molecular Toxicology. 19(1):19-24
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Abstract:

Uroporphyrinogen decarboxylase is an essential enzyme in all organisms and functions in the heme biosynthetic pathway, catalyzing the decarboxylation of the four acetate groups of uroporphyrinogen to form coproporphyrinogen. This work examines whether the four sequential decarboxylations occur at the same active site, and explores whether hexachlorobenzene-induced porphyria affects the behavior of the enzyme. For this purpose, kinetic competition studies were done with mixtures of uroporphyrinogen III and pentacarboxyporphyrinogen III. With the enzyme from normal rats, a constant velocity was obtained with all the mixtures, indicating that uroporphyrinogen and pentacarboxy-porphyrinogen react at the same active site, i.e. the first and fourth decarboxylations occur at the same site. In contrast, in experiments with enzyme from rats with hexachlorobenzene-induced porphyria, the total rate for mixtures was always lower than the reference rate; and a curve with a deep minimum was obtained, indicating that the two reactions occur at functionally different sites, but with cross-inhibition. This suggests that the modifications induced in the enzyme by hexachlorobenzene cause the two active sites to become nonequivalent and functionally different. The question is discussed how the hexachlorobenzene treatment may produce this abnormal kinetic behavior, and alternative hypotheses are considered. © 2005 Wiley Periodicals, Inc.

Registro:

Documento: Artículo
Título:Abnormal kinetic behavior of uroporphyrinogen decarboxylase obtained from rats with hexachlorobenzene-induced porphyria
Autor:Chaufan, G.; Corvi, M.M.; San Martín De Viale, L.C.; Cárdenas, M.L.; Ríos De Molina, M.D.C.
Filiación:Departamento de Quimica Biologica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Lab. Bioenerg./Ingenierie Proteines, Inst. de Biol. Struct. et Microbiol., CNRS, 31 Chemin Joseph-Aiguier, Marseille, France
Palabras clave:Active Site; Competition Plot; Heme Biosynthesis; Hexachlorobenzene; Pentacarboxyporphyrinogen; Porphyria Cutanea Tarda (PCT); Tetrapyr-role; UROD; Uroporphyrinogen Decarboxylase; acetic acid; coproporphyrinogen; hexachlorobenzene; pentacarboxyporphyrinogen; porphyrinogen; unclassified drug; uroporphyrinogen; uroporphyrinogen decarboxylase; animal model; article; chemical reaction; decarboxylation; enzyme active site; enzyme activity; enzyme kinetics; enzyme mechanism; enzyme purification; enzyme structure; nonhuman; porphyria; rat; Animals; Female; Hexachlorobenzene; Kinetics; Liver; Porphyrias; Porphyrinogens; Rats; Rats, Wistar; Uroporphyrinogen Decarboxylase; Uroporphyrinogens
Año:2005
Volumen:19
Número:1
Página de inicio:19
Página de fin:24
DOI: http://dx.doi.org/10.1002/jbt.20055
Título revista:Journal of Biochemical and Molecular Toxicology
Título revista abreviado:J. Biochem. Mol. Toxicol.
ISSN:10956670
CODEN:JBMTF
CAS:acetic acid, 127-08-2, 127-09-3, 64-19-7, 71-50-1; coproporphyrinogen, 37293-37-1; hexachlorobenzene, 118-74-1, 55600-34-5; porphyrinogen, 4396-11-6; uroporphyrinogen decarboxylase, 9024-70-8; uroporphyrinogen, 35465-57-7; Hexachlorobenzene, 118-74-1; pentacarboxylic porphyrinogen; Porphyrinogens; Uroporphyrinogen Decarboxylase, EC 4.1.1.37; Uroporphyrinogens
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10956670_v19_n1_p19_Chaufan

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Citas:

---------- APA ----------
Chaufan, G., Corvi, M.M., San Martín De Viale, L.C., Cárdenas, M.L. & Ríos De Molina, M.D.C. (2005) . Abnormal kinetic behavior of uroporphyrinogen decarboxylase obtained from rats with hexachlorobenzene-induced porphyria. Journal of Biochemical and Molecular Toxicology, 19(1), 19-24.
http://dx.doi.org/10.1002/jbt.20055
---------- CHICAGO ----------
Chaufan, G., Corvi, M.M., San Martín De Viale, L.C., Cárdenas, M.L., Ríos De Molina, M.D.C. "Abnormal kinetic behavior of uroporphyrinogen decarboxylase obtained from rats with hexachlorobenzene-induced porphyria" . Journal of Biochemical and Molecular Toxicology 19, no. 1 (2005) : 19-24.
http://dx.doi.org/10.1002/jbt.20055
---------- MLA ----------
Chaufan, G., Corvi, M.M., San Martín De Viale, L.C., Cárdenas, M.L., Ríos De Molina, M.D.C. "Abnormal kinetic behavior of uroporphyrinogen decarboxylase obtained from rats with hexachlorobenzene-induced porphyria" . Journal of Biochemical and Molecular Toxicology, vol. 19, no. 1, 2005, pp. 19-24.
http://dx.doi.org/10.1002/jbt.20055
---------- VANCOUVER ----------
Chaufan, G., Corvi, M.M., San Martín De Viale, L.C., Cárdenas, M.L., Ríos De Molina, M.D.C. Abnormal kinetic behavior of uroporphyrinogen decarboxylase obtained from rats with hexachlorobenzene-induced porphyria. J. Biochem. Mol. Toxicol. 2005;19(1):19-24.
http://dx.doi.org/10.1002/jbt.20055