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Abstract:

We studied the participation of carbonic anhydrase (CA), V-H +-ATPase, and Cl-/HCO3 - exchanger in electrogenic ion absorption through the gills of Chasmagnathus granulatus. CA activity was measured in anterior gills and posterior gills after acclimation to 2‰, 10‰, 30‰ (about seawater), and 45‰ salinity. The highest CA specific activity was detected in the microsomal fraction in anterior gills, and in the cytosolic fraction, in posterior ones. Both fractions were strongly induced by decreasing salinity only in posterior gills. Perfusion of posterior gills from crabs acclimated to either 2‰ or 10‰ with acetazolamide inhibited CA activity almost completely. In posterior gills from crabs acclimated to 2‰ and perfused with 20‰ saline (iso-osmotic for these crabs), acetazolamide reduced transepithelial potential difference (Vte) by 47%, further addition of ouabain enhanced the effect to 88%. Acetazolamide had no effect in the same gills perfused with 30‰ saline (iso-osmotic for seawater acclimated crabs). Bafilomycin A1 and SITS (inhibitors of V-H+-ATPase and Cl-/HCO3 -) reduced Vte by 15-16% in gills perfused with normal 20‰ saline, and by 77% and 45%, respectively when they were applied in Na-free 20‰ saline, suggesting the participation of those transporters and cytosolic CA in electrogenic ion absorption. © 2005 Elsevier Inc. All rights reserved.

Registro:

Documento: Artículo
Título:Possible role of carbonic anhydrase, V-H+-ATPase, and Cl -/HCO3 - exchanger in electrogenic ion transport across the gills of the euryhaline crab Chasmagnathus granulatus
Autor:Genovese, G.; Ortiz, N.; Urcola, M.R.; Luquet, C.M.
Filiación:Departamento de Biodiversidad Y Biología Experimental, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, (C1428EHA) Buenos Aires, Argentina
CONICET (Consejo Nacional de Investigaciones Científicas Y Técnicas), Rivadavia 1917, (C1033AAJ) Buenos Aires, Argentina
Departamento de Biodiversidad Y Biología Experimental, FCEyN, Ciudad Universitaria, (C1428EHA) Buenos Aires, Argentina
Palabras clave:Carbonic anhydrase; Chasmagnathus granulatus; Cl-/HCO3 - exchanger; Crab; Electrogenic ion transport; Gills; H+-ATPase; Transepithelial potential difference; 4 acetamido 4' isothiocyanatostilbene 2,2' disulfonic acid; acetazolamide; adenosine triphosphatase; bafilomycin A1; carbonate dehydratase; ouabain; absorption; acclimatization; animal tissue; article; controlled study; crab; cytosol; enzyme activity; gill; ion exchange; ion transport; microsome; nonhuman; osmosis; perfusion; salinity; Acetazolamide; Adenosine Triphosphatases; Animals; Biological Transport, Active; Brachyura; Carbonic Anhydrase Inhibitors; Carbonic Anhydrases; Chloride-Bicarbonate Antiporters; Electrophysiology; Gills; Ion Transport; Osmotic Pressure; Ouabain; Sodium Chloride; Animalia; Chasmagnathus granulata; Decapoda (Crustacea); Strophanthus gratus
Año:2005
Volumen:142
Número:3
Página de inicio:362
Página de fin:369
DOI: http://dx.doi.org/10.1016/j.cbpa.2005.08.024
Título revista:Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology
Título revista abreviado:Comp. Biochem. Physiol. A Mol. Integr. Physiol.
ISSN:10956433
CODEN:CBPAB
CAS:4 acetamido 4' isothiocyanatostilbene 2,2' disulfonic acid, 51023-76-8; acetazolamide, 1424-27-7, 59-66-5; adenosine triphosphatase, 37289-25-1, 9000-83-3; bafilomycin A1, 88899-55-2; carbonate dehydratase, 9001-03-0; ouabain, 11018-89-6, 630-60-4; Acetazolamide, 59-66-5; Adenosine Triphosphatases, EC 3.6.1.-; Carbonic Anhydrase Inhibitors; Carbonic Anhydrases, EC 4.2.1.1; Chloride-Bicarbonate Antiporters; Ouabain, 630-60-4; Sodium Chloride, 7647-14-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10956433_v142_n3_p362_Genovese

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Citas:

---------- APA ----------
Genovese, G., Ortiz, N., Urcola, M.R. & Luquet, C.M. (2005) . Possible role of carbonic anhydrase, V-H+-ATPase, and Cl -/HCO3 - exchanger in electrogenic ion transport across the gills of the euryhaline crab Chasmagnathus granulatus. Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology, 142(3), 362-369.
http://dx.doi.org/10.1016/j.cbpa.2005.08.024
---------- CHICAGO ----------
Genovese, G., Ortiz, N., Urcola, M.R., Luquet, C.M. "Possible role of carbonic anhydrase, V-H+-ATPase, and Cl -/HCO3 - exchanger in electrogenic ion transport across the gills of the euryhaline crab Chasmagnathus granulatus" . Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology 142, no. 3 (2005) : 362-369.
http://dx.doi.org/10.1016/j.cbpa.2005.08.024
---------- MLA ----------
Genovese, G., Ortiz, N., Urcola, M.R., Luquet, C.M. "Possible role of carbonic anhydrase, V-H+-ATPase, and Cl -/HCO3 - exchanger in electrogenic ion transport across the gills of the euryhaline crab Chasmagnathus granulatus" . Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology, vol. 142, no. 3, 2005, pp. 362-369.
http://dx.doi.org/10.1016/j.cbpa.2005.08.024
---------- VANCOUVER ----------
Genovese, G., Ortiz, N., Urcola, M.R., Luquet, C.M. Possible role of carbonic anhydrase, V-H+-ATPase, and Cl -/HCO3 - exchanger in electrogenic ion transport across the gills of the euryhaline crab Chasmagnathus granulatus. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 2005;142(3):362-369.
http://dx.doi.org/10.1016/j.cbpa.2005.08.024