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Halperin, J.; Genovese, G.; Tresguerres, M.; Luquet, C.M. "Modulation of ion uptake across posterior gills of the crab Chasmagnathus granulatus by dopamine and cAMP" (2004) Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology. 139(1):103-109
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Abstract:

Cyclic AMP (cAMP) and dopamine modulate ion uptake across isolated and perfused posterior gills of Chasmagnathus granulatus acclimated to 10‰ salinity. Addition of cAMP agonists, such as cp-cAMP, forskolin, and IBMX, produced a significant increase in the transepithelial potential difference (Vte), which reflects ion transport activity. Dopamine (DA) also had a stimulatory effect on ion uptake, increasing Vte and Na+ influx, although this effect was transient, since both variables remained elevated for less than 30 min. In addition, the dose-response curve for DA concentration-Vte was biphasic, and the maximum stimulation was obtained with 10 μmol l -1. When the effects of forskolin and DA on the Na+/K +-ATPase activity were tested, they correlated well with the Vte and Na+ influx experiments; the enzyme activity increased significantly after preincubation of gill fragments for 10 min with forskolin or DA (51 and 64%, respectively), but there was no effect after pre-incubation with DA for 20 min. Finally, KT5720, a specific inhibitor of cAMP-dependent protein kinase (PKA), completely abolished the stimulatory effect of DA on Vte, suggesting the involvement of PKA in this mechanism. © 2004 Elsevier Inc. All rights reserved.

Registro:

Documento: Artículo
Título:Modulation of ion uptake across posterior gills of the crab Chasmagnathus granulatus by dopamine and cAMP
Autor:Halperin, J.; Genovese, G.; Tresguerres, M.; Luquet, C.M.
Filiación:Depto. de Biodiversidad Y Biol. Exp., Fac. de Ciencias Exactas Y Naturales, Univ. Buenos Aires, Pabellon II, C., Argentina
CONICET Rivadavia 1917 Buenos Aires, Argentina, Argentina
Dept. of Physiology and Biophysics, University of Illinois at Chicago, 835 S. Wolcott St., M/C 901, C., United States
Palabras clave:cAMP; Chasmagnathus granulatus; Crab; Dopamine; Gills; Ion transport; PKA; Transepithelial potential differences; adenosine triphosphatase; cyclic AMP; cyclic AMP dependent protein kinase; cyclic AMP dependent protein kinase inhibitor; dopamine; forskolin; ion; isobutylmethylxanthine; kt 5720; potassium ion; sodium ion; article; Chasmagnathus granulatus; concentration (parameters); correlation analysis; crab; dose response; enzyme activity; gill; ion transport; male; nonhuman; potential difference; salinity; sodium transport; Chasmagnathus granulata; Decapoda (Crustacea)
Año:2004
Volumen:139
Número:1
Página de inicio:103
Página de fin:109
DOI: http://dx.doi.org/10.1016/j.cbpb.2004.07.001
Título revista:Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology
Título revista abreviado:Comp. Biochem. Physiol. A Mol. Integr. Physiol.
ISSN:10956433
CODEN:CBPAB
CAS:adenosine triphosphatase, 37289-25-1, 9000-83-3; cyclic AMP, 60-92-4; cyclic AMP dependent protein kinase; dopamine, 51-61-6, 62-31-7; forskolin, 66575-29-9; isobutylmethylxanthine, 28822-58-4; kt 5720, 108068-98-0; potassium ion, 24203-36-9; sodium ion, 17341-25-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10956433_v139_n1_p103_Halperin

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Citas:

---------- APA ----------
Halperin, J., Genovese, G., Tresguerres, M. & Luquet, C.M. (2004) . Modulation of ion uptake across posterior gills of the crab Chasmagnathus granulatus by dopamine and cAMP. Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology, 139(1), 103-109.
http://dx.doi.org/10.1016/j.cbpb.2004.07.001
---------- CHICAGO ----------
Halperin, J., Genovese, G., Tresguerres, M., Luquet, C.M. "Modulation of ion uptake across posterior gills of the crab Chasmagnathus granulatus by dopamine and cAMP" . Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology 139, no. 1 (2004) : 103-109.
http://dx.doi.org/10.1016/j.cbpb.2004.07.001
---------- MLA ----------
Halperin, J., Genovese, G., Tresguerres, M., Luquet, C.M. "Modulation of ion uptake across posterior gills of the crab Chasmagnathus granulatus by dopamine and cAMP" . Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology, vol. 139, no. 1, 2004, pp. 103-109.
http://dx.doi.org/10.1016/j.cbpb.2004.07.001
---------- VANCOUVER ----------
Halperin, J., Genovese, G., Tresguerres, M., Luquet, C.M. Modulation of ion uptake across posterior gills of the crab Chasmagnathus granulatus by dopamine and cAMP. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 2004;139(1):103-109.
http://dx.doi.org/10.1016/j.cbpb.2004.07.001