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Abstract:

We present an experimental and theoretical study of a new scheme for Near-Field Fluorescence Correlation Spectroscopy that, using the field enhancement by optical nanoantennas, allows the reduction of the observation volume 4 orders of magnitude below the diffraction limit. This reduction can be used in two different ways: to increase the sample concentration and to improve the spatial resolution of the dynamics under study. Our experimental results using individual gold nanoparticles and a 150μM Rose Bengal solution in glycerol confirm the volume reduction. © 2008 Optical Society of America.

Registro:

Documento: Artículo
Título:10000 times volume reduction for fluorescence correlation spectroscopy using nano-antennas
Autor:Estrada, L.C.; Aramendía, P.F.; Martínez, O.E.
Filiación:Dpto. de Física. Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428, Buenos Aires, Argentina
INQUIMAE, Departamento de Química Inorgánica, Analítica y Química Física, Universidad de Buenos Aires, 1428, Buenos Aires, Argentina
CONICET. Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina
Palabras clave:Antennas; Diffraction; Fluorescence; Fluorescence spectroscopy; Optical correlation; Spectroscopic analysis; Diffraction limits; Field enhancement; Fluorescence Correlation Spectroscopy; Gold Nanoparticles; Near-field fluorescence; Orders of magnitude; Sample concentration; Spatial resolution; Nanoantennas; nanomaterial; article; chemistry; computer aided design; computer simulation; equipment; equipment design; instrumentation; light; methodology; radiation scattering; reproducibility; sensitivity and specificity; spectrofluorometry; theoretical model; ultrastructure; Computer Simulation; Computer-Aided Design; Equipment Design; Equipment Failure Analysis; Light; Models, Theoretical; Nanostructures; Reproducibility of Results; Scattering, Radiation; Sensitivity and Specificity; Spectrometry, Fluorescence
Año:2008
Volumen:16
Número:25
Página de inicio:20597
Página de fin:20602
DOI: http://dx.doi.org/10.1364/OE.16.020597
Título revista:Optics Express
Título revista abreviado:Opt. Express
ISSN:10944087
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10944087_v16_n25_p20597_Estrada

Referencias:

  • Magde, D., Elson, E., Webb, W.W., Thermodynamic fluctuations in a reacting system-measurement by fluorescence correlation spectroscopy (1972) Phys. Rev. Lett, 29, pp. 705-708
  • Bismuto, E., Gratton, E., Lamb, D.C., Dynamics of ANS Binding to Tuna Apomyoglobin Measured with Fluorescence Correlation. Spectroscopy (2001) Biophys. J, 81, pp. 3510-3521
  • Kastrup, L., Blom, H., Eggeling, C., Hell, S.W., Fluorescence Correlation Spectroscopy in Subdiffraction Focal Volumes (2005) Phys. Rev. Lett, 94, p. 178104
  • Klar, T.A., Jakobs, S., Dyba, M., Egner, A., Hell, S.W., Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission (2000) Proc. Nat. Acad Sci, 97, pp. 8206-8210
  • Rigneault, H., Lenne, P., Fluorescence correlation spectroscopy on a mirror (2003) J. Opt. Soc. Am. B, 20, pp. 2203-2214
  • Starr, T.E., Thompson, N.L., Total Internal Reflection with Fluorescence Correlation Spectroscopy: Combined Surface Reaction and Solution Diffusion (2001) Biophys. J, 80, pp. 1575-1584
  • García-Parajó, M.F., de Bakker, B.I., Koopman, M., Cambi, A., de Lange, F., Figdor, C.G., van Hulst, N.F., Near-Field Fluorescence Microscopy: An optical Nanotool to Study Protein Organization at the Cell Membrane (2005) NanoBiotechnology, 1, pp. 113-120
  • Kawata, Y., Xu, C., Denk, W., Feasibility of molecular-resolution fluorescence near-field microscopy using multi-photon absorption and field enhancenment near a sharp tip (1999) J. Appl. Phys, 85, p. 1294
  • Calander, N., Muthu, P., Gryczynski, Z., Gryczynski, I., Borejdo, J., Fluorescence correlation spectroscopy in a reverse Kretchmann surface plasmon assisted microscope (2008) Opt. Express, 16, pp. 13381-13390. , http://www.opticsinfobase.org/abstract.cfm?URI=oe-16-17-13381
  • Borejdo, J., Calander, N., Gryczynski, Z., Gryczynski, I., Fluorescence correlation spectroscopy in surface plasmon coupled emission microscope (2006) Opt. Express, 14, pp. 7878-7888. , http://www.opticsinfobase.org/abstract.cfm?URI=oe-14-17-7878
  • Foquet, M., Korlach, J., Zipfel, W.R., Webb, W.W., Craighead, H.G., Focal Volume Confinement by Submicrometer-Sized Fluidic Channels (2004) Anal. Chem, 76, pp. 1618-1626
  • Levene, M.J., Korlach, J., Turner, S.W., Foquet, M., Craighead, H.G., Webb, W.W., Zero-Mode Waveguides for Single-Molecule Analysis at High Concentrations (2003) Science, 299, pp. 682-686
  • Leutenegger, M., Gösch, M., Perentes, A., Hoffmann, P., Martin, O.J.F., Lasser, T., Confining the sampling volume for Fluorescence Correlation Spectroscopy using a sub-wavelength sized aperture (2006) Opt. Express, 14, pp. 956-969. , http://www.opticsinfobase.org/abstract.cfm?URI=oe-14-2-956
  • Wenger, J., Gérard, D., Lenne, P., Rigneault, H., Dintinger, J., Ebbesen, T.W., Boned, A., Marguet, D., Dual-color fluorescence cross-correlation spectroscopy in a single nanoaperture : Towards rapid multicomponent screening at high concentrations (2006) Opt. Express, 14, pp. 12206-12216. , http://www.opticsinfobase.org/abstract.cfm?URI=oe-14-25-12206
  • Rigneault, H., Capoulade, J., Dintinger, J., Wenger, J., Bonod, N., Popov, E., Ebbesen, T.W., Lenne, P.F., Enhancement of Single-Molecule Fluorescence Detection in Subwavelength Apertures (2005) Phys. Rev. Lett, 95, p. 117401
  • (2002) Single-Molecule Detection in Solution-Methods and Applications, , C. Zander, J. Enderlein, R. A. Keller ed, Wiley-VCH, Chap. 3
  • Fradin, C., Abu-Arish, A., Granek, R., Elbaum, M., Fluorescence Correlation Spectroscopy Close to a Fluctuating Membrane (2003) Biophys. J, 84, pp. 2005-2020
  • Novotny, L., Hecht, B., (2007) Principles of Nano-Optics, , Cambridge University Press
  • Tam, F., Goodrich, G.P., Johnson, B.R., Halas, N.J., Plasmonic Enhancement of Molecular Fluorescence (2007) Nano Lett, 7, pp. 496-501
  • Aslan, K., Gryczynski, I., Malicka, J., Matveeva, E., Lakowicz, J.R., Geddes, C.D., Metal-enhanced fluorescence: An emerging tool in biotechnology (2005) Curr. Op. in Biotech, 16, pp. 55-62
  • Muskens, O.L., Giannini, V., Sánchez-Gil, J.A., Gómez Rivas, J., Strong Enhancement of the Radiative Decay Rate of Emitters by Single Plasmonic Nanoantennas (2007) Nano Lett, 7, pp. 2871-2875
  • Anger, P., Bharadwaj, P., Novotny, L., Enhancement and Quenching of Single-Molecule Fluorescence (2006) Phys. Rev. Lett, 96, p. 113002
  • Bharadwaj, P., Novotny, L., Spectral dependence of single molecule fluorescence enhancement (2007) Opt. Express, 15, pp. 14266-14274. , http://www.opticsinfobase.org/abstract.cfm?URI=oe-15-21-14266

Citas:

---------- APA ----------
Estrada, L.C., Aramendía, P.F. & Martínez, O.E. (2008) . 10000 times volume reduction for fluorescence correlation spectroscopy using nano-antennas. Optics Express, 16(25), 20597-20602.
http://dx.doi.org/10.1364/OE.16.020597
---------- CHICAGO ----------
Estrada, L.C., Aramendía, P.F., Martínez, O.E. "10000 times volume reduction for fluorescence correlation spectroscopy using nano-antennas" . Optics Express 16, no. 25 (2008) : 20597-20602.
http://dx.doi.org/10.1364/OE.16.020597
---------- MLA ----------
Estrada, L.C., Aramendía, P.F., Martínez, O.E. "10000 times volume reduction for fluorescence correlation spectroscopy using nano-antennas" . Optics Express, vol. 16, no. 25, 2008, pp. 20597-20602.
http://dx.doi.org/10.1364/OE.16.020597
---------- VANCOUVER ----------
Estrada, L.C., Aramendía, P.F., Martínez, O.E. 10000 times volume reduction for fluorescence correlation spectroscopy using nano-antennas. Opt. Express. 2008;16(25):20597-20602.
http://dx.doi.org/10.1364/OE.16.020597