Abstract:
We present an experimental and theoretical study of a new scheme for Near-Field Fluorescence Correlation Spectroscopy that, using the field enhancement by optical nanoantennas, allows the reduction of the observation volume 4 orders of magnitude below the diffraction limit. This reduction can be used in two different ways: to increase the sample concentration and to improve the spatial resolution of the dynamics under study. Our experimental results using individual gold nanoparticles and a 150μM Rose Bengal solution in glycerol confirm the volume reduction. © 2008 Optical Society of America.
Registro:
Documento: |
Artículo
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Título: | 10000 times volume reduction for fluorescence correlation spectroscopy using nano-antennas |
Autor: | Estrada, L.C.; Aramendía, P.F.; Martínez, O.E. |
Filiación: | Dpto. de Física. Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428, Buenos Aires, Argentina INQUIMAE, Departamento de Química Inorgánica, Analítica y Química Física, Universidad de Buenos Aires, 1428, Buenos Aires, Argentina CONICET. Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina
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Palabras clave: | Antennas; Diffraction; Fluorescence; Fluorescence spectroscopy; Optical correlation; Spectroscopic analysis; Diffraction limits; Field enhancement; Fluorescence Correlation Spectroscopy; Gold Nanoparticles; Near-field fluorescence; Orders of magnitude; Sample concentration; Spatial resolution; Nanoantennas; nanomaterial; article; chemistry; computer aided design; computer simulation; equipment; equipment design; instrumentation; light; methodology; radiation scattering; reproducibility; sensitivity and specificity; spectrofluorometry; theoretical model; ultrastructure; Computer Simulation; Computer-Aided Design; Equipment Design; Equipment Failure Analysis; Light; Models, Theoretical; Nanostructures; Reproducibility of Results; Scattering, Radiation; Sensitivity and Specificity; Spectrometry, Fluorescence |
Año: | 2008
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Volumen: | 16
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Número: | 25
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Página de inicio: | 20597
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Página de fin: | 20602
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DOI: |
http://dx.doi.org/10.1364/OE.16.020597 |
Título revista: | Optics Express
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Título revista abreviado: | Opt. Express
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ISSN: | 10944087
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10944087_v16_n25_p20597_Estrada |
Referencias:
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Citas:
---------- APA ----------
Estrada, L.C., Aramendía, P.F. & Martínez, O.E.
(2008)
. 10000 times volume reduction for fluorescence correlation spectroscopy using nano-antennas. Optics Express, 16(25), 20597-20602.
http://dx.doi.org/10.1364/OE.16.020597---------- CHICAGO ----------
Estrada, L.C., Aramendía, P.F., Martínez, O.E.
"10000 times volume reduction for fluorescence correlation spectroscopy using nano-antennas"
. Optics Express 16, no. 25
(2008) : 20597-20602.
http://dx.doi.org/10.1364/OE.16.020597---------- MLA ----------
Estrada, L.C., Aramendía, P.F., Martínez, O.E.
"10000 times volume reduction for fluorescence correlation spectroscopy using nano-antennas"
. Optics Express, vol. 16, no. 25, 2008, pp. 20597-20602.
http://dx.doi.org/10.1364/OE.16.020597---------- VANCOUVER ----------
Estrada, L.C., Aramendía, P.F., Martínez, O.E. 10000 times volume reduction for fluorescence correlation spectroscopy using nano-antennas. Opt. Express. 2008;16(25):20597-20602.
http://dx.doi.org/10.1364/OE.16.020597