Artículo

Bianchi, V.A.; Rocchetta, I.; Luquet, C.M. "Biomarker responses to sewage pollution in freshwater mussels (Diplodon chilensis) transplanted to a Patagonian river" (2014) Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering. 49(11):1276-1285
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Abstract:

Field and laboratory experiments were combined to evaluate biomarker responses of Diplodon chilensis to sewage pollution. Mussels from an unpolluted area in Lacar lake (S0) were caged at a reference site (S1) and at two sites with increasing sewage pollution (S2, S3) in Pocahullo river (all in Argentina). After 1 month, gill (g) glutathione S-transferase (GST) and catalase (CAT) activities, and lipid peroxidation (TBARS) were found to be significantly elevated in S3, gGST being positively correlated with fecal bacteria (FC) concentration. Digestive gland (dg) enzyme activities were depressed and dgTBARS were increased in all transplanted mussels. After 3 mo, most variables returned to control levels in S1 mussels except for dgCAT and dgTBARS. After seven months, GST and CAT activities of S0 and S3 mussels were evaluated in the laboratory, before and after acute exposure (8 h) to high fecal bacteria concentration ([FC] in S3x 2). gGST increased in both groups, while dgGST responded only in S3 mussels. gCAT and dgCAT activities were similarly increased by acute exposure in both groups. Our results suggest that gGST and gCAT are suitable biomarkers for high FC pollution regardless of previous exposure history. In addition, we show that dgCAT is sensitive to the acute increase in FC load, both in naive and long-term exposed individuals, while dgGST becomes responsive after long-term acclimatization. © 2014 Copyright © Taylor & Francis Group, LLC.

Registro:

Documento: Artículo
Título:Biomarker responses to sewage pollution in freshwater mussels (Diplodon chilensis) transplanted to a Patagonian river
Autor:Bianchi, V.A.; Rocchetta, I.; Luquet, C.M.
Filiación:Laboratorio de Ecotoxicología Acuática, INIBIOMA (CONICET-UNCo) - CEAN, Ruta provincial N-61, km 3, CCP 7, Neuquen, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:detoxifying defenses; Fecal coliforms; oxidative stress; short and long-term response.; Biomarkers; Coliform bacteria; Molluscs; Oxidative stress; Pollution; Sewage; detoxifying defenses; Fecal coliforms; Freshwater mussels; Glutathione-S-transferase; Laboratory experiments; Lipid peroxidation; short and long-term response; Transplanted mussels; River pollution; biological marker; catalase; glutathione transferase; thiobarbituric acid reactive substance; biological marker; catalase; glutathione transferase; sewage; water pollutant; adult; animal tissue; article; controlled study; Diplodon chilensis; environmental exposure; enzyme activity; exocrine gland; feces microflora; gill; lake; lipid peroxidation; mussel; nonhuman; oxidative stress; river; sewage; sewage pollution; water pollution; analysis; animal; Argentina; bivalve; chemistry; environmental monitoring; metabolism; sewage; water pollutant; water pollution; Animals; Argentina; Biological Markers; Bivalvia; Catalase; Environmental Monitoring; Glutathione Transferase; Lipid Peroxidation; Rivers; Sewage; Water Pollutants, Chemical; Water Pollution, Chemical
Año:2014
Volumen:49
Número:11
Página de inicio:1276
Página de fin:1285
DOI: http://dx.doi.org/10.1080/10934529.2014.910065
Título revista:Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
Título revista abreviado:J. Environ. Sci. Health Part A Toxic Hazard. Subst. Environ. Eng.
ISSN:10934529
CODEN:JATEF
CAS:catalase, 9001-05-2; glutathione transferase, 50812-37-8; Biological Markers; Catalase; Glutathione Transferase; Sewage; Water Pollutants, Chemical
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10934529_v49_n11_p1276_Bianchi

Referencias:

  • Moles, A., Hale, N., Use of physiological responses in Mytilus trossulus as integrative bioindicators of sewage pollution (2003) Marine Pollution Bulletin, 46 (8), pp. 954-958. , DOI 10.1016/S0025-326X(03)00108-5
  • Gagne, F., Blaise, C., Hellou, J., Endocrine disruption and health effects of caged mussels, Elliptio complanata, placed downstream from a primary-treated municipal effluent plume for 1 year (2004) Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, 138 (1), pp. 33-44. , DOI 10.1016/j.cca.2004.04.006, PII S1532045604000778
  • Calabretta, C.J., Oviatt, C.A., The response of benthic macrofauna to anthropogenic stress in Narragansett Bay, Rhode Island: A review of human stressors and assessment of community conditions (2008) Mar. Pollut. Bull, 56, pp. 1680-1695
  • Nicholson, S., Lam, P.K.S., Pollution monitoring in Southeast Asia using biomarkers in the mytilid mussel Perna viridis (Mytilidae: Bivalvia) (2005) Environment International, 31 (1), pp. 121-132. , DOI 10.1016/j.envint.2004.05.007, PII S0160412004000959
  • Viarengo, A., Lowe, D., Bolognesi, C., Fabbri, E., Koehler, A., The use of biomarkers in biomonitoring: A 2-tier approach assessing the level of pollutant-induced stress syndrome in sentinel organisms (2007) Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, 146 (3), pp. 281-300. , DOI 10.1016/j.cbpc.2007.04.011, PII S1532045607001329
  • Mahmoud, N., Dellali, M., El Bour, M., Aissa, P., Mahmoudi, E., The use of Fulvia fragilis (Mollusca: Cardiidae) in the biomonitoring of Bizerta lagoon: A mutimarkers approach (2010) Ecol. Indic, 10, pp. 696-702
  • Tsangaris, C., Kormas, K., Strogyloudi, E., Hatzianestis, I., Neofitou, C., Andral, B., Galgani, F., Multiple biomarkers of pollution effects in caged mussels on the Greek coastline (2010) Comp. Biochem. Physiol. C, 151, pp. 369-378
  • Ribeiro Guevara, S., Arribere, M., Bubach, D., Vigliano, P., Rizzo, A., Alonso, M., Sanchez, R., Silver contamination on abiotic and biotic compartments of Nahuel Huapi National Park lakes, Patagonia, Argentina (2005) Science of the Total Environment, 336 (1-3), pp. 119-134. , DOI 10.1016/j.scitotenv.2004.05.020, PII S0048969704004310
  • Sabatini, S.E., Rocchetta, I., Nahabedian, D.E., Luquet, C.M., Eppis, M.R., Bianchi, L., Ríos De Molina, M.C., Oxidative stress and histological alterations produced by dietary copper in the fresh water bivalve Diplodon chilensis (2011) Comp. Biochem. Physiol. C, 154, pp. 391-398
  • Lau, P.S., Wong, H.L., Garrigues, Ph., Seasonal variation in antioxidative responses and acetylcholinesterase activity in Perna viridis in eastern oceanic and western estuarine waters of Hong Kong (2004) Continental Shelf Research, 24 (16), pp. 1969-1987. , DOI 10.1016/j.csr.2004.06.019, PII S0278434304001347, Pearl River Estuary Study
  • Bocchetti, R., Regoli, F., Seasonal variability of oxidative biomarkers, lysosomal parameters, metallothioneins and peroxisomal enzymes in the Mediterranean mussel Mytilus galloprovincialis from Adriatic Sea (2006) Chemosphere, 65 (6), pp. 913-921. , DOI 10.1016/j.chemosphere.2006.03.049, PII S0045653506003596
  • Box, A., Sureda, A., Galgani, F., Pons, A., Deudero, S., Assessment of environmental pollution at Balearic Islands applying oxidative stress biomarkers in the mussel Mytilus galloprovincialis (2007) Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, 146 (4), pp. 531-539. , DOI 10.1016/j.cbpc.2007.06.006, PII S1532045607001676
  • Sabatini, S.E., Brena, B.M., Luquet, C.M., San Julian, M., Pirez, M., Ríos De Molina, M.C., Microcystin accumulation and antioxidant responses in the freshwater clam Diplodon chilensis patagonicus upon subchronic exposure to toxic Microcystis aeruginosa (2011) Ecotox. Environ. Safe, 74 (5), pp. 1188-1194
  • Sabatini, S.E., Rocchetta, I., Luquet, C.M., Guido, M.I., Ríos De Molina, M.C., Effects of sewage pollution and bacterial load on growth and oxidative balance in the freshwater mussel Diplodon chilensis (2011) Limnologica, 41, pp. 356-362
  • Fraga, C.G., Leibovitz, B.E., Tappel, A.L., Lipid peroxidation measured as thiobarbituric acid-reactive substances in tissue slices. Characterization and comparison with homogenates and microsomes (1988) Free Radical Biology and Medicine, 4 (3), pp. 155-161. , DOI 10.1016/0891-5849(88)90023-8
  • Ceulemans, H., Bollen, M., Functional Diversity of Protein Phosphatase-1, a Cellular Economizer and Reset Button (2004) Physiological Reviews, 84 (1), pp. 1-39. , DOI 10.1152/physrev.00013.2003
  • Chakraborty, S., Ray, M., Ray, S., Toxicity of sodium arsenite in the gill of an economically important mollusc of India (2010) Fish. Shellfish. Immun, 29 (1), pp. 136-148
  • Smolders, R., Bervoets, L., De Coen, W., Blust, R., Cellular energy allocation in zebra mussels exposed along a pollution gradient: Linking cellular effects to higher levels of biological organization (2004) Environmental Pollution, 129 (1), pp. 99-112. , DOI 10.1016/j.envpol.2003.09.027
  • Bainy, A.C.D., Almeida, E.A., Müller, I.C., Ventura, E.C., Medeiros, I.D., Biochemical responses in farmed mussel Perna perna transplanted to contaminated sites on Santa Catarina Island, SC, Brazil (2000) Mar. Environ. Res, 50, pp. 411-416
  • Bergayou, H., Mouneyrac, C., Pellerin, J., Moukrim, A., Oxidative stress responses in bivalves (Scrobicularia plana, Cerastoderma edule) from the Oued Souss estuary (Morocco) (2009) Ecotox. Environ. Saf, 72, pp. 765-769
  • Pedrozo, F., Chillrud, S., Temporetti, P., Díaz, M., Chemical composition and nutrient limitation in rivers and lakes of northern Patagonian Andes (39.5″-429s; 7 1%′) (Rep Argentina) (1993) Int. Ver. Theor. Angew. Limnol. Verh, 25, pp. 207-214
  • Miserendino, M.L., Casaux, R., Archangelsky, M., Di Prinzio, C.Y., Brand, C., Kutschker, A.M., Assessing land-use effects on water quality, in-stream habitat, riparian ecosystems and biodiversity in Patagonian northwest streams (2011) Sci. Total. Environ, 409 (3), pp. 612-624
  • Temporetti, P., Baffico, G., Diaz, M., Pedrozo, F., (2009) Estado Trófico Del Lago Lacar y Del Arroyo Pocahullo. Influencia de la Descarga de Líquidos Cloacales en la Cuenca Oriental Del Lago, , Technical report: Un puente entre la Universidad y la Sociedad. Secretaría de Políticas Universitarias, Ministerio de Educación Ciencia y Tecnología, Universidad Nacional del Comahue: San Carlos de Bariloche, Neuquén, Argentina
  • Bonetto, A.A., Nayades de la Patagonia (1973) Rev. Asoc. Cienc. Nat. Litoral, 4, pp. 117-185
  • Valdovinos, C., Pedreros, P., Geographic variations in shell growth rates of the mussel Diplodon chilensis from temperate lakes of Chile: Implications for biodiversity conservation (2007) Limnologica, 37 (1), pp. 63-75. , DOI 10.1016/j.limno.2006.08.007, PII S007595110600082X, Lymnology of Temperate South America
  • Rocchetta, I., Lomovasky, B., Yusseppone, M.S., Sabatini, S.E., Bieczynski, F., Ríos De Molina, M.C., Luquet, C.M., Growth, abundance, morphometric and metabolic parameters of three populations of freshwater bivalve subject to different levels of natural and anthropogenic organic matter input in a glaciar lake of North Patagonia (2014) Limnologica, 44, pp. 72-80
  • Multiple-tube fermentation technique for members of the coliform group (1998) Standard Methods for Examination of Water and Wastewater, , American Public Health Association (APHA). In, 20th ed. Clesceri L.S. Greenberg, A.E. Eaton, A.D. Eds; APHA, American Water Works Association, and Water Environment Federation: Madrid, Spain, section 9221
  • Lichtenthaler, H.K., Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes (1987) Meth.Enzymol, 148, pp. 350-382
  • Juhel, G., Davenport, J., O'Halloran, J., Culloty, S.C., O'Riordan, R.M., James, K.F., Furey, A., Allis, O., Impacts of microcystins on the feeding behaviour and energy balance of zebra mussels, Dreissena polymorpha: A bioenergetics approach (2006) Aquatic Toxicology, 79 (4), pp. 391-400. , DOI 10.1016/j.aquatox.2006.07.007, PII S0166445X06002955
  • Habig, W.H., Pabst, M.J., Jakoby, W.B., Glutathione S-transferases: The first enzymatic step in mercapturic acid formation (1974) J. Biol. Chem, 249, pp. 7130-7139
  • Aebi, H., Catalase in vitro. Meth (1984) Enzymol, 105, pp. 121-126
  • Carmichael, W.W., An, J., Using an enzyme linked immunosorbent assay (ELISA) and a protein phosphatase inhibition assay (PPIA) for the detection of microcystins and nodularins (1999) Natural Toxins, 7 (6), pp. 377-385. , DOI 10.1002/1522-7189(199911/12)7:6<377::AID-NT80>3.0.CO;2-8
  • Bradford, M., A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal.Biochem, 72, pp. 248-254
  • Cossu, C., Doyotte, A., Babut, M., Exinger, A., Vasseur, P., Antioxidant biomarkers in freshwater bivalves, Unio tumidus, in response to different contamination profiles of aquatic sediments (2000) Ecotoxicology and Environmental Safety, 45 (2), pp. 106-121. , DOI 10.1006/eesa.1999.1842
  • Contardo-Jara, V., Wiegand, C., Molecular biomarkers of Dreissena polymorpha for evaluation of renaturation success of a formerly sewage polluted stream (2008) Environmental Pollution, 155 (1), pp. 182-189. , DOI 10.1016/j.envpol.2007.10.018, PII S0269749107005118
  • Farcy, E., Gagné, F., Martel, L., Fortier, M., Trépanier, S., Brousseau, P., Fournier, M., Short-term physiological effects of a xenobiotic mixture on the freshwater mussel Elliptio complanata exposed to municipal effluents (2011) Environ. Res, 111, pp. 1096-1106
  • Faria, M., López, M.A., Díez, S., Barata, C., Are native naiads more tolerant to pollution than exotic freshwater bivalve species? An hypothesis tested using physiological responses of three species transplanted to mercury contaminated sites in the Ebro River (NE, Spain) (2010) Chemosphere, 81, pp. 1218-1226
  • Albentosa, M., Fernandez-Reiriz, M.J., Labarta, U., Perez-Camacho, A., Response of two species of clams, Ruditapes decussatus and Venerupis pullastra, to starvation: Physiological and biochemical parameters (2007) Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 146 (2), pp. 241-249. , DOI 10.1016/j.cbpb.2006.10.109, PII S1096495906003782
  • Albentosa, M., Sánchez-Hernández, M., Campillo, J.A., Moyano, F.J., Relationship between physiological measurements (SFG-scope for growth-) and the functionality of the digestive gland in Mytilus galloprovincialis (2012) Comp. Biochem. Physiol. A, 163 (34), pp. 286-295
  • Camus, L., Pampanin, D.M., Volpato, E., Delaney, E., Sanni, S., Nasci, C., Total oxyradical scavenging capacity responses in Mytilus galloprovincialis transplanted into the Venice lagoon (Italy) to measure the biological impact of anthropogenic activities (2004) Marine Pollution Bulletin, 49 (9-10), pp. 801-808. , DOI 10.1016/j.marpolbul.2004.06.009, PII S0025326X04002024
  • Ellis, J., Cummings, V., Hewitt, J., Thrush, S., Norkko, A., Determining effects of suspended sediment on condition of a suspension feeding bivalve (Atrina zelandica): Results of a survey, a laboratory experiment and a field transplant experiment (2002) Journal of Experimental Marine Biology and Ecology, 267 (2), pp. 147-174. , DOI 10.1016/S0022-0981(01)00355-0, PII S0022098101003550
  • Regoli, F., Principato, G., Glutathione, glutathione-dependent and antioxidant enzymes in mussel, Mytilus galloprovincialis, exposed to metals under field and laboratory conditions: Implications for the use of biochemical biomarkers (1995) Aquat. Toxicol, 31 (2), pp. 143-164
  • Franzellitti, S., Buratti, S., Donnini, F., Fabbri, E., Exposure of mussels to a polluted environment: Insights into the stress syndrome development (2010) Comp. Biochem. Physiol. C, 152, pp. 24-33
  • Gagné, F., André, C., Fortier, M., Fournier, M., Immunotoxic potential of aeration lagoon effluents for the treatment of domestic and hospital wastewaters in the freshwater mussel Elliptio complanata (2012) J. Environ. Sci, 24, pp. 781-789
  • Lushchak, V.I., Environmentally induced oxidative stress in aquatic animals (2011) Aquat.Toxicol, 101, pp. 13-30
  • Tsung-Meng, W., Yuan-Ting, H., Ming-Shiuan, S., Yi-Ting, H., Tse-Min, L., Expression of genes involved in redox homeostasis and antioxidant defense in a marine macroalga Ulva fasciata by excess copper (2009) Aquat. Toxicol, 94, pp. 275-285
  • Gust, M., Fortier, M., Garric, J., Fournier, M., Gagné, F., Immunotoxicity of surface waters contaminated by municipal effluents to the snail Lymnaea stagnalis (2013) Aquat. Toxicol, 126, pp. 393-403

Citas:

---------- APA ----------
Bianchi, V.A., Rocchetta, I. & Luquet, C.M. (2014) . Biomarker responses to sewage pollution in freshwater mussels (Diplodon chilensis) transplanted to a Patagonian river. Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering, 49(11), 1276-1285.
http://dx.doi.org/10.1080/10934529.2014.910065
---------- CHICAGO ----------
Bianchi, V.A., Rocchetta, I., Luquet, C.M. "Biomarker responses to sewage pollution in freshwater mussels (Diplodon chilensis) transplanted to a Patagonian river" . Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering 49, no. 11 (2014) : 1276-1285.
http://dx.doi.org/10.1080/10934529.2014.910065
---------- MLA ----------
Bianchi, V.A., Rocchetta, I., Luquet, C.M. "Biomarker responses to sewage pollution in freshwater mussels (Diplodon chilensis) transplanted to a Patagonian river" . Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering, vol. 49, no. 11, 2014, pp. 1276-1285.
http://dx.doi.org/10.1080/10934529.2014.910065
---------- VANCOUVER ----------
Bianchi, V.A., Rocchetta, I., Luquet, C.M. Biomarker responses to sewage pollution in freshwater mussels (Diplodon chilensis) transplanted to a Patagonian river. J. Environ. Sci. Health Part A Toxic Hazard. Subst. Environ. Eng. 2014;49(11):1276-1285.
http://dx.doi.org/10.1080/10934529.2014.910065