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Abstract:

In the present study, the effects on oxidative balance and cellular end points of glyphosate, aminomethylphosphonic acid (AMPA), and a glyphosate formulation (G formulation) were examined in HepG2 cell line, at dilution levels far below agricultural recommendations. Our results show that G formulation had toxic effects while no effects were found with acid glyphosate and AMPA treatments. Glyphosate formulation exposure produced an increase in reactive oxygen species, nitrotyrosine formation, superoxide dismutase activity, and glutathione (GSH) levels, while no effects were observed for catalase and GSH-S-transferase activities. Also, G formulation triggered caspase 3/7 activation and hence induced apoptosis pathway in this cell line. Aminomethylphosphonic acid exposure produced an increase in GSH levels while no differences were observed in other antioxidant parameters. No effects were observed when the cells were exposed to acid glyphosate. These results confirm that G formulations have adjuvants working together with the active ingredient and causing toxic effects that are not seen with acid glyphosate. © The Author(s) 2014.

Registro:

Documento: Artículo
Título:Glyphosate commercial formulation causes cytotoxicity, oxidative effects, and apoptosis on human cells: Differences with its active ingredient
Autor:Chaufan, G.; Coalova, I.; Del Carmen Ríos De Molina, M.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:apoptosis; cytotoxicity; glyphosate; in vtro; oxidative stress; apoptosis; cytotoxicity; glyphosate; in vtro; oxidative stress; Adjuvants, Pharmaceutic; Apoptosis; Chemistry, Pharmaceutical; Drug Synergism; Glutathione; Glycine; Hep G2 Cells; Hepatocytes; Herbicides; Humans; Lethal Dose 50; Organophosphonates; Osmolar Concentration; Oxidation-Reduction; Oxidative Stress; Reactive Oxygen Species; Superoxide Dismutase; Tyrosine; Up-Regulation
Año:2014
Volumen:33
Número:1
Página de inicio:29
Página de fin:38
DOI: http://dx.doi.org/10.1177/1091581813517906
Título revista:International Journal of Toxicology
Título revista abreviado:Int. J. Toxicol.
ISSN:10915818
CODEN:IJTOF
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10915818_v33_n1_p29_Chaufan

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Citas:

---------- APA ----------
Chaufan, G., Coalova, I. & Del Carmen Ríos De Molina, M. (2014) . Glyphosate commercial formulation causes cytotoxicity, oxidative effects, and apoptosis on human cells: Differences with its active ingredient. International Journal of Toxicology, 33(1), 29-38.
http://dx.doi.org/10.1177/1091581813517906
---------- CHICAGO ----------
Chaufan, G., Coalova, I., Del Carmen Ríos De Molina, M. "Glyphosate commercial formulation causes cytotoxicity, oxidative effects, and apoptosis on human cells: Differences with its active ingredient" . International Journal of Toxicology 33, no. 1 (2014) : 29-38.
http://dx.doi.org/10.1177/1091581813517906
---------- MLA ----------
Chaufan, G., Coalova, I., Del Carmen Ríos De Molina, M. "Glyphosate commercial formulation causes cytotoxicity, oxidative effects, and apoptosis on human cells: Differences with its active ingredient" . International Journal of Toxicology, vol. 33, no. 1, 2014, pp. 29-38.
http://dx.doi.org/10.1177/1091581813517906
---------- VANCOUVER ----------
Chaufan, G., Coalova, I., Del Carmen Ríos De Molina, M. Glyphosate commercial formulation causes cytotoxicity, oxidative effects, and apoptosis on human cells: Differences with its active ingredient. Int. J. Toxicol. 2014;33(1):29-38.
http://dx.doi.org/10.1177/1091581813517906