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Abstract:

In this work the antiviral activity of 20 dehydroepiandrosterone (DHEA) analogs with different substituents at positions C-3, C-15, C-16 and C-17 were evaluated against vesicular stomatitis virus (VSV) in Vero cell cultures. The selectivity indexes (SI) obtained with DHEA and epiandrosterone (EA) were 50 and 72.6, respectively. The work showed that the compounds 21-norpregna-5,17(20)-dien-3β,16α-diyl-diacetate, 17,17-ethylendioxyandrostan-5,15-dien-3β-ol and 3β-hydroxypregn-17(20)-en-16-one had higher SI values than ribavirin, which was used as a reference drug. The antiviral mode of action of DHEA was also investigated against VSV replication in Vero cells, and time of addition experiments showed that DHEA mainly affected a late event in the virus growth cycle. Analysis of RNA and protein synthesis indicated that DHEA adversely affected positive strand RNA synthesis and viral mature particle formation. © 2008 Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:In vitro antiviral activity of dehydroepiandrosterone and its synthetic derivatives against vesicular stomatitis virus
Autor:Romanutti, C.; Bruttomesso, A.C.; Castilla, V.; Bisceglia, J.A.; Galagovsky, L.R.; Wachsman, M.B.
Filiación:Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellon 2, Piso 4, C1428EGA Buenos Aires, Argentina
Departamento de Química Orgánica, UMYMFOR, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellon 2, Piso 4, C1428EGA Buenos Aires, Argentina
Palabras clave:Antiviral; Dehydroepiandrosterone analogs; DHEA; EA; Vesicular stomatitis virus; 1 beta dextro ribofuranosil 1 2 4 triazole 3 carboxamide; 16alpha hydroxyprasterone; 17 oxo 5alpha androstan 3beta,16alpha yldiacetate; 17 oxo 5alpha androstan 3beta,16beta yldiacetate; 17 oxoandrost 5 en 3beta yl acetate; 17 oxoandrost 5 en 3beta ylacetae; 17 oxoandrost 5 en 3beta,16alpha diyldiacetate; 17 oxoandrost 5 en 3beta,16beta yldiacetate; 17,17 ethylendioxy 3beta androstanyl acetate; 17,17 ethylendioxyandrost 5 en 3beta ol; 17,17 ethylendioxyandrost 5,15 dien 3beta ol; 17,17 ethylendioxyandrostan 16alpha bromo 3beta ol; 17,17 ethylendioxyandrostan 3beta ol; 17,17 ethylendioxyandrostan 5,15 dien 3beta ol; 21 norpregna 5,17(20) dien 3beta,16alpha diyldiacetate; 3beta 6alpha dihydroxy 17 androstanone; 3beta hydroxypregn 17(20) en 16 one; 3beta,15alpha dihydroxy 5alpha androstan 17 one; 3beta,15beta dihydroxy 5alpha androstan 17 one; 3beta,16alpha dihydroxy 5alpha androstan 17 one; androsta 5,16 dien 3beta,17 diyldiacetate; antivirus agent; epiandrosterone; prasterone; prasterone derivative; ribavirin; unclassified drug; animal cell; antiviral activity; article; controlled study; drug mechanism; growth inhibition; in vitro study; nonhuman; protein synthesis; RNA synthesis; Vero cell; Vesicular stomatitis virus; virogenesis; virus particle; virus replication; Animals; Antiviral Agents; Cercopithecus aethiops; Dehydroepiandrosterone; Fluorescent Antibody Technique, Indirect; Reverse Transcriptase Polymerase Chain Reaction; RNA, Viral; Vero Cells; Vesicular Stomatitis; Vesiculovirus; Virus Replication; Vesicular stomatitis virus
Año:2009
Volumen:182
Número:2
Página de inicio:327
Página de fin:335
DOI: http://dx.doi.org/10.1016/j.tvjl.2008.06.015
Título revista:Veterinary Journal
Título revista abreviado:Vet. J.
ISSN:10900233
CODEN:VTJRF
CAS:epiandrosterone, 481-29-8; prasterone, 53-43-0; ribavirin, 36791-04-5; Antiviral Agents; Dehydroepiandrosterone, 53-43-0; RNA, Viral
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10900233_v182_n2_p327_Romanutti

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Citas:

---------- APA ----------
Romanutti, C., Bruttomesso, A.C., Castilla, V., Bisceglia, J.A., Galagovsky, L.R. & Wachsman, M.B. (2009) . In vitro antiviral activity of dehydroepiandrosterone and its synthetic derivatives against vesicular stomatitis virus. Veterinary Journal, 182(2), 327-335.
http://dx.doi.org/10.1016/j.tvjl.2008.06.015
---------- CHICAGO ----------
Romanutti, C., Bruttomesso, A.C., Castilla, V., Bisceglia, J.A., Galagovsky, L.R., Wachsman, M.B. "In vitro antiviral activity of dehydroepiandrosterone and its synthetic derivatives against vesicular stomatitis virus" . Veterinary Journal 182, no. 2 (2009) : 327-335.
http://dx.doi.org/10.1016/j.tvjl.2008.06.015
---------- MLA ----------
Romanutti, C., Bruttomesso, A.C., Castilla, V., Bisceglia, J.A., Galagovsky, L.R., Wachsman, M.B. "In vitro antiviral activity of dehydroepiandrosterone and its synthetic derivatives against vesicular stomatitis virus" . Veterinary Journal, vol. 182, no. 2, 2009, pp. 327-335.
http://dx.doi.org/10.1016/j.tvjl.2008.06.015
---------- VANCOUVER ----------
Romanutti, C., Bruttomesso, A.C., Castilla, V., Bisceglia, J.A., Galagovsky, L.R., Wachsman, M.B. In vitro antiviral activity of dehydroepiandrosterone and its synthetic derivatives against vesicular stomatitis virus. Vet. J. 2009;182(2):327-335.
http://dx.doi.org/10.1016/j.tvjl.2008.06.015