Artículo
Gensch, T.; Böhmer, M.; Aramendía, P.F. "Single molecule blinking and photobleaching separated by wide-field fluorescence microscopy" (2005) Journal of Physical Chemistry A. 109(30):6652-6658
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Abstract:
Single molecule fluorescence detection of Atto590 in poly(vinyl alcohol) was achieved by using a wide-field epifluorescence microscope with CCD-camera detection. Image sequences are obtained from which the time traces of the detected molecules are built. We find a distinctive difference between the time evolution of the fluorescence originating from the molecules detected in the first image of the sequence compared to the time evolution of the fluorescence of the molecules detected in each image of the sequence. Atto590 shows very long blinking times and photobleaching and photoblinking that are both quadratically dependent on the irradiation power density. Our approach allows kinetic separation of photobleaching from blinking. The possibility of choosing different ensembles of molecules is demonstrated and taken advantage of for this aim. Initially dark molecules or low emitting ones that might be overlooked are important to describe the complete ensemble behavior. © 2005 American Chemical Society.
Registro:
| Documento: | Artículo |
| Título: | Single molecule blinking and photobleaching separated by wide-field fluorescence microscopy |
| Autor: | Gensch, T.; Böhmer, M.; Aramendía, P.F. |
| Filiación: | Institute for Biological Information Processing, IBI-1, Research Centre Jülich GmbH, 52425 Jülich, Germany INQUIMAE, Departamento de Química Inorgánica, Ciudad Universitaria, Pabellón 2, C1428EHA Buenos Aires, Argentina |
| Palabras clave: | Charge coupled devices; Electron transitions; Excitons; Fluorescence; Irradiation; Molecular dynamics; Molecular structure; Ensemble behavior; Epifluorescence detection; Photobleaching; Single molecule fluorescence detection; Organic compounds; Atto590; fluorescent dye; fused heterocyclic rings; polyvinyl alcohol; solvent; article; bleaching; chemical structure; chemistry; fluorescence microscopy; methodology; Fluorescent Dyes; Heterocyclic Compounds with 4 or More Rings; Microscopy, Fluorescence; Molecular Structure; Photobleaching; Polyvinyl Alcohol; Solvents |
| Año: | 2005 |
| Volumen: | 109 |
| Número: | 30 |
| Página de inicio: | 6652 |
| Página de fin: | 6658 |
| DOI: | http://dx.doi.org/10.1021/jp0510847 |
| Título revista: | Journal of Physical Chemistry A |
| Título revista abreviado: | J Phys Chem A |
| ISSN: | 10895639 |
| CODEN: | JPCAF |
| CAS: | polyvinyl alcohol, 37380-95-3, 9002-89-5; Atto590; Fluorescent Dyes; Heterocyclic Compounds with 4 or More Rings; Polyvinyl Alcohol, 9002-89-5; Solvents |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10895639_v109_n30_p6652_Gensch |
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Citas:
---------- APA ----------
Gensch, T., Böhmer, M. & Aramendía, P.F. (2005) . Single molecule blinking and photobleaching separated by wide-field fluorescence microscopy. Journal of Physical Chemistry A, 109(30), 6652-6658.http://dx.doi.org/10.1021/jp0510847
---------- CHICAGO ----------
Gensch, T., Böhmer, M., Aramendía, P.F. "Single molecule blinking and photobleaching separated by wide-field fluorescence microscopy" . Journal of Physical Chemistry A 109, no. 30 (2005) : 6652-6658.http://dx.doi.org/10.1021/jp0510847
---------- MLA ----------
Gensch, T., Böhmer, M., Aramendía, P.F. "Single molecule blinking and photobleaching separated by wide-field fluorescence microscopy" . Journal of Physical Chemistry A, vol. 109, no. 30, 2005, pp. 6652-6658.http://dx.doi.org/10.1021/jp0510847
---------- VANCOUVER ----------
Gensch, T., Böhmer, M., Aramendía, P.F. Single molecule blinking and photobleaching separated by wide-field fluorescence microscopy. J Phys Chem A. 2005;109(30):6652-6658.http://dx.doi.org/10.1021/jp0510847
