Artículo

Lidke, D.S.; Nagy, P.; Heintzmann, R.; Arndt-Jovin, D.J.; Post, J.N.; Grecco, H.E.; Jares-Erijman, E.A.; Jovin, T.M. "Quantum dot ligands provide new insights into erbB/HER receptor-mediated signal transduction" (2004) Nature Biotechnology. 22(2):198-203
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Abstract:

The erbB/HER family of transmembrane receptor tyrosine kinases (RTKs) mediate cellular responses to epidermal growth factor (EGF) and related ligands. We have imaged the early stages of RTK-dependent signaling in living cells using: (i) stable expression of erbB1/2/3 fused with visible fluorescent proteins (VFPs), (ii) fluorescent quantum dots (QDs) bearing epidermal growth factor (EGF-QD) and (iii) continuous confocal laser scanning microscopy and flow cytometry. Here we demonstrate that EGF-QDs are highly specific and potent in the binding and activation of the EGF receptor (erbB1), being rapidly internalized into endosomes that exhibit active trafficking and extensive fusion. EGF-QDs bound to erbB1 expressed on filopodia revealed a previously unreported mechanism of retrograde transport to the cell body. When erbB2-monomeric yellow fluorescent protein (mYFP) or erbB3-monomeric Citrine (mCitrine) were coexpressed with erbB1, the rates and extent of endocytosis of EGF-QD and the RTK-VFP demonstrated that erbB2 but not erbB3 heterodimerizes with erbB1 after EGF stimulation, thereby modulating EGF-induced signaling. QD-ligands will find widespread use in basic research and biotechnological developments.

Registro:

Documento: Artículo
Título:Quantum dot ligands provide new insights into erbB/HER receptor-mediated signal transduction
Autor:Lidke, D.S.; Nagy, P.; Heintzmann, R.; Arndt-Jovin, D.J.; Post, J.N.; Grecco, H.E.; Jares-Erijman, E.A.; Jovin, T.M.
Filiación:Department of Molecular Biology, Max Planck Inst. for Biophys. Chem., Am Fassberg 11, D-37077 Göttingen, Germany
Departamento de Física, Ciudad Universitaria, Universidad de Buenos Aires, C1428EHA Buenos Aires, Argentina
Departamento de Quimica Organica, Universidad de Buenos Aires, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina
Palabras clave:Cells; Enzymes; Semiconductor quantum dots; Epidermal growth factors (EGF); Biotechnology; citrine; epidermal growth factor; epidermal growth factor receptor; epidermal growth factor receptor 2; epidermal growth factor receptor 3; fluorescent dye; hybrid protein; ligand; monomer; oligomer; protein tyrosine kinase; unclassified drug; yellow fluorescent protein; animal cell; article; biotechnology; cell line; cell strain A431; CHO cell; confocal laser microscopy; controlled study; dimerization; endocytosis; endosome; filopodium; flow cytometry; fluorescence; human; human cell; internalization; ligand binding; nonhuman; priority journal; quantitative analysis; quantum mechanics; signal transduction; Animals; Cell Membrane; Cricetinae; Endosomes; Epidermal Growth Factor; Humans; Motion; Oncogene Proteins v-erbB; Protein Binding; Protein Interaction Mapping; Protein Transport; Quantum Dots; Receptors, Cell Surface; Signal Transduction; Spectrometry, Fluorescence; Animalia
Año:2004
Volumen:22
Número:2
Página de inicio:198
Página de fin:203
DOI: http://dx.doi.org/10.1038/nbt929
Título revista:Nature Biotechnology
Título revista abreviado:Nat. Biotechnol.
ISSN:10870156
CODEN:NABIF
CAS:citrine, 14832-92-9; epidermal growth factor receptor 2, 137632-09-8; epidermal growth factor receptor 3, 497121-54-7; epidermal growth factor, 62229-50-9; protein tyrosine kinase, 80449-02-1; Epidermal Growth Factor, 62229-50-9; Oncogene Proteins v-erbB; Receptors, Cell Surface
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10870156_v22_n2_p198_Lidke

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Citas:

---------- APA ----------
Lidke, D.S., Nagy, P., Heintzmann, R., Arndt-Jovin, D.J., Post, J.N., Grecco, H.E., Jares-Erijman, E.A.,..., Jovin, T.M. (2004) . Quantum dot ligands provide new insights into erbB/HER receptor-mediated signal transduction. Nature Biotechnology, 22(2), 198-203.
http://dx.doi.org/10.1038/nbt929
---------- CHICAGO ----------
Lidke, D.S., Nagy, P., Heintzmann, R., Arndt-Jovin, D.J., Post, J.N., Grecco, H.E., et al. "Quantum dot ligands provide new insights into erbB/HER receptor-mediated signal transduction" . Nature Biotechnology 22, no. 2 (2004) : 198-203.
http://dx.doi.org/10.1038/nbt929
---------- MLA ----------
Lidke, D.S., Nagy, P., Heintzmann, R., Arndt-Jovin, D.J., Post, J.N., Grecco, H.E., et al. "Quantum dot ligands provide new insights into erbB/HER receptor-mediated signal transduction" . Nature Biotechnology, vol. 22, no. 2, 2004, pp. 198-203.
http://dx.doi.org/10.1038/nbt929
---------- VANCOUVER ----------
Lidke, D.S., Nagy, P., Heintzmann, R., Arndt-Jovin, D.J., Post, J.N., Grecco, H.E., et al. Quantum dot ligands provide new insights into erbB/HER receptor-mediated signal transduction. Nat. Biotechnol. 2004;22(2):198-203.
http://dx.doi.org/10.1038/nbt929