Abstract:
The erbB/HER family of transmembrane receptor tyrosine kinases (RTKs) mediate cellular responses to epidermal growth factor (EGF) and related ligands. We have imaged the early stages of RTK-dependent signaling in living cells using: (i) stable expression of erbB1/2/3 fused with visible fluorescent proteins (VFPs), (ii) fluorescent quantum dots (QDs) bearing epidermal growth factor (EGF-QD) and (iii) continuous confocal laser scanning microscopy and flow cytometry. Here we demonstrate that EGF-QDs are highly specific and potent in the binding and activation of the EGF receptor (erbB1), being rapidly internalized into endosomes that exhibit active trafficking and extensive fusion. EGF-QDs bound to erbB1 expressed on filopodia revealed a previously unreported mechanism of retrograde transport to the cell body. When erbB2-monomeric yellow fluorescent protein (mYFP) or erbB3-monomeric Citrine (mCitrine) were coexpressed with erbB1, the rates and extent of endocytosis of EGF-QD and the RTK-VFP demonstrated that erbB2 but not erbB3 heterodimerizes with erbB1 after EGF stimulation, thereby modulating EGF-induced signaling. QD-ligands will find widespread use in basic research and biotechnological developments.
Registro:
Documento: |
Artículo
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Título: | Quantum dot ligands provide new insights into erbB/HER receptor-mediated signal transduction |
Autor: | Lidke, D.S.; Nagy, P.; Heintzmann, R.; Arndt-Jovin, D.J.; Post, J.N.; Grecco, H.E.; Jares-Erijman, E.A.; Jovin, T.M. |
Filiación: | Department of Molecular Biology, Max Planck Inst. for Biophys. Chem., Am Fassberg 11, D-37077 Göttingen, Germany Departamento de Física, Ciudad Universitaria, Universidad de Buenos Aires, C1428EHA Buenos Aires, Argentina Departamento de Quimica Organica, Universidad de Buenos Aires, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina
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Palabras clave: | Cells; Enzymes; Semiconductor quantum dots; Epidermal growth factors (EGF); Biotechnology; citrine; epidermal growth factor; epidermal growth factor receptor; epidermal growth factor receptor 2; epidermal growth factor receptor 3; fluorescent dye; hybrid protein; ligand; monomer; oligomer; protein tyrosine kinase; unclassified drug; yellow fluorescent protein; animal cell; article; biotechnology; cell line; cell strain A431; CHO cell; confocal laser microscopy; controlled study; dimerization; endocytosis; endosome; filopodium; flow cytometry; fluorescence; human; human cell; internalization; ligand binding; nonhuman; priority journal; quantitative analysis; quantum mechanics; signal transduction; Animals; Cell Membrane; Cricetinae; Endosomes; Epidermal Growth Factor; Humans; Motion; Oncogene Proteins v-erbB; Protein Binding; Protein Interaction Mapping; Protein Transport; Quantum Dots; Receptors, Cell Surface; Signal Transduction; Spectrometry, Fluorescence; Animalia |
Año: | 2004
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Volumen: | 22
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Número: | 2
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Página de inicio: | 198
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Página de fin: | 203
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DOI: |
http://dx.doi.org/10.1038/nbt929 |
Título revista: | Nature Biotechnology
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Título revista abreviado: | Nat. Biotechnol.
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ISSN: | 10870156
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CODEN: | NABIF
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CAS: | citrine, 14832-92-9; epidermal growth factor receptor 2, 137632-09-8; epidermal growth factor receptor 3, 497121-54-7; epidermal growth factor, 62229-50-9; protein tyrosine kinase, 80449-02-1; Epidermal Growth Factor, 62229-50-9; Oncogene Proteins v-erbB; Receptors, Cell Surface
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10870156_v22_n2_p198_Lidke |
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Citas:
---------- APA ----------
Lidke, D.S., Nagy, P., Heintzmann, R., Arndt-Jovin, D.J., Post, J.N., Grecco, H.E., Jares-Erijman, E.A.,..., Jovin, T.M.
(2004)
. Quantum dot ligands provide new insights into erbB/HER receptor-mediated signal transduction. Nature Biotechnology, 22(2), 198-203.
http://dx.doi.org/10.1038/nbt929---------- CHICAGO ----------
Lidke, D.S., Nagy, P., Heintzmann, R., Arndt-Jovin, D.J., Post, J.N., Grecco, H.E., et al.
"Quantum dot ligands provide new insights into erbB/HER receptor-mediated signal transduction"
. Nature Biotechnology 22, no. 2
(2004) : 198-203.
http://dx.doi.org/10.1038/nbt929---------- MLA ----------
Lidke, D.S., Nagy, P., Heintzmann, R., Arndt-Jovin, D.J., Post, J.N., Grecco, H.E., et al.
"Quantum dot ligands provide new insights into erbB/HER receptor-mediated signal transduction"
. Nature Biotechnology, vol. 22, no. 2, 2004, pp. 198-203.
http://dx.doi.org/10.1038/nbt929---------- VANCOUVER ----------
Lidke, D.S., Nagy, P., Heintzmann, R., Arndt-Jovin, D.J., Post, J.N., Grecco, H.E., et al. Quantum dot ligands provide new insights into erbB/HER receptor-mediated signal transduction. Nat. Biotechnol. 2004;22(2):198-203.
http://dx.doi.org/10.1038/nbt929