Artículo
Rossi, J.P.F.C.; Villamil, A.M.; Echarte, M.M.; Alzugaray, M.E.; Borelli, M.I.; García, M.E.; Pande, J.; Grover, A.K.; Gagliardino, J.J. "Plasma membrane calcium pump activity in rat pancreatic islets: An accurate method to measure its calcium-dependent modulation" (2006) Cell Biochemistry and Biophysics. 46(3):193-200
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Abstract:
The aim of this study was to quantify the glucose modulation of the plasma membrane calcium pump (PMCA) function in rat pancreatic islets. Ca2+-ATPase activity and levels of phosphorylated PMCA intermediates both transiently declined to a minimum in response to stimulation by glucose. Strictly dependent on Ca2+ concentration, this inhibitory effect was fully expressed at physiological concentrations of the cation (less than 0.5 μM), then progressively diminished at higher concentrations. These results, together with those previously reported on the effects of insulin secretagogues and blockers on the activity, expression and cellular distribution of the PMCA, support the concept that the PMCA plays a key role in the regulation of Ca2+ signaling and insulin secretion in pancreatic islets. © Copyright 2006 by Humana Press Inc. All rights of any nature whatsoever reserved.
Registro:
| Documento: | Artículo |
| Título: | Plasma membrane calcium pump activity in rat pancreatic islets: An accurate method to measure its calcium-dependent modulation |
| Autor: | Rossi, J.P.F.C.; Villamil, A.M.; Echarte, M.M.; Alzugaray, M.E.; Borelli, M.I.; García, M.E.; Pande, J.; Grover, A.K.; Gagliardino, J.J. |
| Filiación: | Departamento de Química Biológica, Instituto de Química Y Fisicoquímica Biológicas, UBA-CONICET, Buenos Aires, Argentina Centro de Endocrinología Experimental Y Aplicada (UNLP-CONICET, Centro Colaborador OPS/OMS), Facultad de Ciencias Médicas, UNLP, La Plata, Argentina Department of Medicine, HSC 4N41, McMaster University, 1200 Main St. West, Hamilton, Ont. L8N 3Z5, Canada |
| Palabras clave: | Insulin secretion; Islet plasma membrane Ca2+ pump; Normal rat islets; Phosphorylation; Plasma membrane Ca2+-ATPases; PMCA isoforms; calcium; glucose; isoenzyme; plasma membrane calcium transporting adenosine triphosphatase; unclassified drug; animal; article; cell membrane; channel gating; drug effect; enzymology; in vitro study; male; pancreas islet; phosphorylation; physiology; rat; Wistar rat; Animals; Calcium; Cell Membrane; Glucose; Ion Channel Gating; Islets of Langerhans; Isoenzymes; Male; Phosphorylation; Plasma Membrane Calcium-Transporting ATPases; Rats; Rats, Wistar; Rattus |
| Año: | 2006 |
| Volumen: | 46 |
| Número: | 3 |
| Página de inicio: | 193 |
| Página de fin: | 200 |
| DOI: | http://dx.doi.org/10.1385/CBB:46:3:193 |
| Título revista: | Cell Biochemistry and Biophysics |
| Título revista abreviado: | Cell Biochem. Biophys. |
| ISSN: | 10859195 |
| CODEN: | CBBIF |
| CAS: | calcium, 7440-70-2; glucose, 50-99-7, 84778-64-3; Calcium, 7440-70-2; Glucose, 50-99-7; Isoenzymes; Plasma Membrane Calcium-Transporting ATPases, 3.6.3.8 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10859195_v46_n3_p193_Rossi |
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Citas:
---------- APA ----------
Rossi, J.P.F.C., Villamil, A.M., Echarte, M.M., Alzugaray, M.E., Borelli, M.I., García, M.E., Pande, J.,..., Gagliardino, J.J. (2006) . Plasma membrane calcium pump activity in rat pancreatic islets: An accurate method to measure its calcium-dependent modulation. Cell Biochemistry and Biophysics, 46(3), 193-200.http://dx.doi.org/10.1385/CBB:46:3:193
---------- CHICAGO ----------
Rossi, J.P.F.C., Villamil, A.M., Echarte, M.M., Alzugaray, M.E., Borelli, M.I., García, M.E., et al. "Plasma membrane calcium pump activity in rat pancreatic islets: An accurate method to measure its calcium-dependent modulation" . Cell Biochemistry and Biophysics 46, no. 3 (2006) : 193-200.http://dx.doi.org/10.1385/CBB:46:3:193
---------- MLA ----------
Rossi, J.P.F.C., Villamil, A.M., Echarte, M.M., Alzugaray, M.E., Borelli, M.I., García, M.E., et al. "Plasma membrane calcium pump activity in rat pancreatic islets: An accurate method to measure its calcium-dependent modulation" . Cell Biochemistry and Biophysics, vol. 46, no. 3, 2006, pp. 193-200.http://dx.doi.org/10.1385/CBB:46:3:193
---------- VANCOUVER ----------
Rossi, J.P.F.C., Villamil, A.M., Echarte, M.M., Alzugaray, M.E., Borelli, M.I., García, M.E., et al. Plasma membrane calcium pump activity in rat pancreatic islets: An accurate method to measure its calcium-dependent modulation. Cell Biochem. Biophys. 2006;46(3):193-200.http://dx.doi.org/10.1385/CBB:46:3:193
