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Abstract:

A simple reversed phase HPLC method was developed for the determination of polyamines (putrescine, cadaverine, spermidine, spermine) and other polycations (n-acetyl putrescine, histamine, n-acetilspermine, agmatine), as their benzoylated derivatives. The advantages of this method, examined in various matrixes (hypothalamus, hypophyses, ovary, and seminal plasma), are its versatility, simplicity, and short time of determination of polyamines and related substances of importance in prokaryotes and eukaryotes, even in different matrixes, using a standard HPLC equipment (UV detection at 254 nm), with good resolution, sensitivity, and reproducibility.

Registro:

Documento: Artículo
Título:An improved HPLC method for identification and quantitation of polyamines and related compounds as benzoylated derivatives
Autor:Hockl, P.F.; Thyssen, S.M.; Libertun, C.
Filiación:Lab. Neuroendocrinología, Instituto de Biología y Medicina Experimental-CONICET, Dep.Fisiologia, Facultad de Medicina, UBA V. Obligado, Buenos Aires 2490 (1428), Argentina
Lab. Neuroendocrinología, Instituto de Biología y Medicina Experimental-CONICET, Dep.Fisiologia, Facultad de Medicina, UBA V. Obligado, Buenos Aires 2490 (1428), Argentina
Palabras clave:agmatine; cadaverine; histamine; polyamine; polycation; putrescine; putrescine derivative; spermidine; spermine; spermine derivative; animal tissue; article; calibration; chromatography; derivatization; female; high performance liquid chromatography; hypophysis; hypothalamus; nonhuman; ovary; polyamine synthesis; rat; reproducibility; reversed phase high performance liquid chromatography; seminal plasma; standard
Año:2000
Volumen:23
Número:5
Página de inicio:693
Página de fin:703
DOI: http://dx.doi.org/10.1081/JLC-100101482
Título revista:Journal of Liquid Chromatography and Related Technologies
Título revista abreviado:J. Liq. Chromatogr. Relat. Technol.
ISSN:10826076
CAS:agmatine, 306-60-5; cadaverine, 1476-39-7, 462-94-2; histamine, 51-45-6, 56-92-8, 93443-21-1; putrescine, 110-60-1, 333-93-7; spermidine, 124-20-9, 334-50-9; spermine, 306-67-2, 71-44-3
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10826076_v23_n5_p693_Hockl

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Citas:

---------- APA ----------
Hockl, P.F., Thyssen, S.M. & Libertun, C. (2000) . An improved HPLC method for identification and quantitation of polyamines and related compounds as benzoylated derivatives. Journal of Liquid Chromatography and Related Technologies, 23(5), 693-703.
http://dx.doi.org/10.1081/JLC-100101482
---------- CHICAGO ----------
Hockl, P.F., Thyssen, S.M., Libertun, C. "An improved HPLC method for identification and quantitation of polyamines and related compounds as benzoylated derivatives" . Journal of Liquid Chromatography and Related Technologies 23, no. 5 (2000) : 693-703.
http://dx.doi.org/10.1081/JLC-100101482
---------- MLA ----------
Hockl, P.F., Thyssen, S.M., Libertun, C. "An improved HPLC method for identification and quantitation of polyamines and related compounds as benzoylated derivatives" . Journal of Liquid Chromatography and Related Technologies, vol. 23, no. 5, 2000, pp. 693-703.
http://dx.doi.org/10.1081/JLC-100101482
---------- VANCOUVER ----------
Hockl, P.F., Thyssen, S.M., Libertun, C. An improved HPLC method for identification and quantitation of polyamines and related compounds as benzoylated derivatives. J. Liq. Chromatogr. Relat. Technol. 2000;23(5):693-703.
http://dx.doi.org/10.1081/JLC-100101482