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Abstract:

Gregarines that parasitise phlebotomine sand flies belong to the genus Psychodiella and, even though they are highly host-specific, only five species have been described to date. Their most outstanding features include the unique localisation of the oocysts in the accessory glands of the female host, which ensures contamination of the egg surface during oviposition, and the fact that they naturally parasitise the vectors of Leishmania, causal agent of leishmaniasis. The type species, Ps. chagasi, was first described in Lutzomyia longipalpis, vector of visceral leishmaniasis (VL), from Brazil. We recently reported Ps. chagasi sequences in Lu. longipalpis from Posadas (Misiones, Argentina), an endemic VL location where this gregarine had not been previously recorded. In order to analyse the incidence of Ps. chagasi infections in Lu. longipalpis from this location, the aim of this study was to develop a diagnostic assay for sand fly gregarine parasites in Lu. longipalpis. For this, we designed primers using the Ps. chagasi sequences we previously identified and performed an in vitro validation by PCR amplification of the original sand fly samples. Their specificity and sensitivity as diagnostic primers were subsequently confirmed by PCR reactions using total DNA extracted from naturally infected Lu. longipalpis from the same location (Posadas, Argentina). © 2014 The Society for Vector Ecology.

Registro:

Documento: Artículo
Título:Polymerase chain reaction-based assay for the detection and identification of sand fly gregarines in Lutzomyia longipalpis, a vector of visceral leishmaniasis
Autor:Caligiuri, L.G.; Acardi, S.A.; Soledad Santini, M.; Salomón, O.D.; Mccarthy, C.B.
Filiación:Centro Regional de Estudios Genómicos, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Florencio Varela, Argentina
Departamento de Informática y Tecnología, Universidad Nacional del Noroeste de la Provincia de Buenos Aires, Pergamino, Buenos Aires, Argentina
Laboratorio de Biología Molecular Aplicada, Facultad de Ciencias Exactas, Químicas y Naturales, Universidad Nacional de Misiones, Posadas, Misiones, Argentina
Centro Nacional de Diagnóstico e Investigación en Endemoepidemias, Administración Nacional de Laboratorios e Institutos de Salud, Ministerio de Salud, Buenos Aires, Argentina
Instituto Nacional de Medicina Tropical, Ministerio de Salud de la Nación, Puerto Iguazú, Misiones, Argentina
Palabras clave:Diagnostic primers; Lutzomyia longipalpis; PCR-based assay; Psychodiella chagasi; Sand fly gregarines; Visceral leishmaniasis (VL); animal; Argentina; disease carrier; female; parasitology; Phlebotomus; polymerase chain reaction; procedures; Psychodidae; transmission; visceral leishmaniasis; Animals; Argentina; Female; Insect Vectors; Leishmaniasis, Visceral; Phlebotomus; Polymerase Chain Reaction; Psychodidae
Año:2014
Volumen:39
Número:1
Página de inicio:83
Página de fin:93
DOI: http://dx.doi.org/10.1111/j.1948-7134.2014.12074.x
Título revista:Journal of Vector Ecology
Título revista abreviado:J. Vector Ecol.
ISSN:10811710
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10811710_v39_n1_p83_Caligiuri

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Citas:

---------- APA ----------
Caligiuri, L.G., Acardi, S.A., Soledad Santini, M., Salomón, O.D. & Mccarthy, C.B. (2014) . Polymerase chain reaction-based assay for the detection and identification of sand fly gregarines in Lutzomyia longipalpis, a vector of visceral leishmaniasis. Journal of Vector Ecology, 39(1), 83-93.
http://dx.doi.org/10.1111/j.1948-7134.2014.12074.x
---------- CHICAGO ----------
Caligiuri, L.G., Acardi, S.A., Soledad Santini, M., Salomón, O.D., Mccarthy, C.B. "Polymerase chain reaction-based assay for the detection and identification of sand fly gregarines in Lutzomyia longipalpis, a vector of visceral leishmaniasis" . Journal of Vector Ecology 39, no. 1 (2014) : 83-93.
http://dx.doi.org/10.1111/j.1948-7134.2014.12074.x
---------- MLA ----------
Caligiuri, L.G., Acardi, S.A., Soledad Santini, M., Salomón, O.D., Mccarthy, C.B. "Polymerase chain reaction-based assay for the detection and identification of sand fly gregarines in Lutzomyia longipalpis, a vector of visceral leishmaniasis" . Journal of Vector Ecology, vol. 39, no. 1, 2014, pp. 83-93.
http://dx.doi.org/10.1111/j.1948-7134.2014.12074.x
---------- VANCOUVER ----------
Caligiuri, L.G., Acardi, S.A., Soledad Santini, M., Salomón, O.D., Mccarthy, C.B. Polymerase chain reaction-based assay for the detection and identification of sand fly gregarines in Lutzomyia longipalpis, a vector of visceral leishmaniasis. J. Vector Ecol. 2014;39(1):83-93.
http://dx.doi.org/10.1111/j.1948-7134.2014.12074.x