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The quick detection of domestic and peridomestic triatomines in their environments becomes difficult without the use of dislodgement substances that flush them out from their shelters. At present, tetramethrin 0.2% is being widely used in control programs. Although it is an efficient dislodging agent, its toxicity might affect the health of captured triatomines, of other insects and, to a lesser extent, of other animals, including humans. Here, we tested if semiochemicals released by disturbed adults of Triatoma infestans and/or Rhodnius prolixus can make larvae of the same species exit from their refuges. In a walking olfactometer we found that: 1) larvae of T. infestans were repelled by the odors released by disturbed adults of their own species and of R. prolixus, 2) larvae of R. prolixus did not change their behavior in the presence of odors released by adults of both species, and 3) activity levels were not modulated by these odors in any of both species. Besides, in pseudo-natural conditions we found an increased flushing-out activity of larvae of T. infestans when their shelters were sprayed with isobutyric acid or 3-pentanol, and of larvae of R. prolixus when sprayed with 3-methyl-1-butanol. We succeeded in this work to dislodge larvae of triatomines from artificial shelters using natural volatile compounds, allowing the capture of live bugs for further investigations (e.g., xenodiagnosis or genetic studies) and favoring ecological aspects (e.g., minimizing environmental insecticide-contamination and non-targeted mortality). © 2013 The Society for Vector Ecology.


Documento: Artículo
Título:Dislodgement effect of natural semiochemicals released by disturbed triatomines: A possible alternative monitoring tool
Autor:Minoli, S.; Palottini, F.; Crespo, J.G.; Manrique, G.
Filiación:Laboratorio de Fisiología de Insectos, Departamento de Biodiversidad y Biologia Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IBBEA, CONICET-UBA, Ciudad Autonoma de Buenos Aires, Argentina
Department of Biology, University of Utah, Salt Lake City, UT, United States
Palabras clave:Behavior; Chagas; Detection; Dislodgement; Exocrine glands; Triatomines; pheromone; animal; article; behavior; Chagas; Chagas disease; Detection; disease carrier; disease transmission; dislodgement; exocrine gland; metabolism; Triatoma; Triatomines; behavior; Chagas; detection; dislodgement; exocrine glands; Triatomines; Animals; Chagas Disease; Insect Vectors; Pheromones; Triatoma
Página de inicio:353
Página de fin:360
Título revista:Journal of Vector Ecology
Título revista abreviado:J. Vector Ecol.


  • Audino, P.G., Alzogaray, R.A., Vassena, C., Masuh, H., Fontan, A., Gatti, P., Martinez, A., Zerba, E., Volatile compounds secreted by Brindley's glands of adult Triatoma infestans: identification and biological activity of previously unidentified compounds (2007) J. Vect. Ecol., 32, pp. 75-82
  • Bohman, B., Troger, A., Franke, S., Lorenzo, M.G., Francke, W., Unelius, C.R., Structure elucidation and synthesis of dioxolanes emitted by two Triatoma species (Hemiptera: Reduviidae) (2011) J Nat Prod, 74, pp. 690-694
  • Brindley, M.D.H., On the metasternal scent-glands of certain Heteroptera (1930) Trans. R. Entomol. Soc. Lond., 78, pp. 199-208
  • Crespo, J.G., Manrique, G., Mating behavior of the hematophagous bug Triatoma infestans: Role of Brindley's and metasternal glands (2007) J. Insect Physiol., 53, pp. 708-714
  • Cruz-López, L., Morgan, E.D., Ondarza, R.N., Brindley's gland exocrine products of Triatoma infestans (1995) Med. Vet. Entomol., 9, pp. 403-406
  • (2010) Environmental protection agency, ,, EPA. Registration eligibility decision
  • Fontan, A., Audino, P.G., Martinez, A., Alzogaray, R.A., Zerb, E.N., Camps, F., Cork, A., Attractant volatiles released by female and male Triatoma infestans (Hemiptera: Reduviidae), a vector of Chagas disease: chemical analysis and behavioral bioassay (2002) J. Med. Entomol., 39, pp. 191-197
  • Garcia-Zapata, M.T., Schofield, C., Marsden, P.D., A simple method to detect the presence of live triatomine bugs in houses sprayed with residual insecticides (1985) T. Roy. Soc. Trop. Med. H., 79, pp. 558-559
  • Garcia-Zapata, M.T., Virgens, D., Marsden, P.D., Comparison of vigilance methods in three houses with different Triatoma infestans densities (1985) Rev. Soc. Bras. Med. Trop., 18, pp. 183-186
  • Guerenstein, P.G., Guerin, P.M., A comparison of volatiles emitted by adults of three triatomine species (2004) Entomol. Exp. Appl., 111, pp. 151-155
  • Gurtler, R.E., Chuit, R., Cecere, M.C., Castanera, M.B., Detecting domestic vectors of Chagas disease: a comparative trial of six methods in north-west Argentina (1995) Bull. Wld. Hlth. Org., 73, pp. 487-494
  • Gurtler, R.E., Schweigmann, N.J., Cecere, M.C., Chuit, R., Wisnivesky-Colli, C., Comparison of two sampling methods for domestic populations of Triatoma infestans in north-west Argentina (1993) Med. Vet. Entomol., 7, pp. 238-242
  • Hack, W.H., Ricciardi, A.I., Oscherov, B., Olivetti de Bravi, M.G., [Composition of the secretion of Brindley's gland in Triatominae] (1980) Medicina (B Aires) 40 Suppl, 1, pp. 178-180
  • Juarez, P., Brenner, R.R., Biochemistry of the evolutive cycle of Triatoma infestans (vinchuca). V. Volatile fatty acids emission (1981) Acta Physiol. Pharm. L., 31, pp. 113-117
  • Kälin, M., Barrett, F.M., Observations on the anatomy, histology, release site, and function of Brindley's glands in the blood-sucking bug, Rhodnius prolixus (Heteroptera: Reduviidae) (1975) Ann. Entomol. Soc. Am., 68, pp. 126-134
  • Lazzari, C.R., Circadian organization of locomotion activity in the haematophagous bug Triatoma infestans (1992) J. Insect Physiol., 38, pp. 895-903
  • Lent, H., Wygodzinsky, P., A revision of the Triatominae (Hemiptera; Reduviidae) and their significance as vectors of Chagas' Disease (1979) B. Am. Mus. Nat. Hist., 163, pp. 1-520
  • Lorenzo, M.G., Lazzari, C.R., Activity pattern in relation to refuge exploitation and feeding in Triatoma infestans (Hemiptera: Reduviidae) (1998) Acta Trop., 70, pp. 163-170
  • Manrique, G., Lorenzo, M.G., The sexual behavior of Chagas' Disease vectors: chemical signals mediating communication between male and female Triatomine bugs (2012) Psyche, 2012, pp. 1-8. , doi: 10.1155/2012/862891
  • Manrique, G., Vitta, A.C., Ferreira, R.A., Zani, C.L., Unelius, C.R., Lazzari, C.R., Diotaiuti, L., Lorenzo, M.G., Chemical communication in Chagas disease vectors. Source, identity, and potential function of volatiles released by the metasternal and Brindley's glands of Triatoma infestans adults (2006) J. Chem. Ecol., 32, pp. 2035-2052
  • Pinchin, R., Fanara, D.M., Castleton, C.W., Oliveira Filho, A.M., Comparison of techniques for detection of domestic infestations with Triatoma infestans in Brazil (1981) Trans. R. Soc. Trop. Med. Hyg., 75, pp. 691-694
  • Pontes, G., Bohman, B., Unelius, C.R., Lorenzo, M., Metasternal gland volatiles and sexual communication in the triatomine bug, Rhodnius prolixus (2008) J. Chem. Ecol., 34, pp. 450-457
  • Pontes, G., Lorenzo, M.G., Female metasternal gland odors mediate male aggregation in Rhodnius prolixus, a triatomid bug (2012) Med. Vet. Entomol., 26, pp. 33-36
  • Rojas, J.C., Rios-Candelaria, E., Cruz-Lopez, L., Santiesteban, A., Bond-Compean, J.G., Brindis, Y., Malo, E.A., A reinvestigation of Brindley's gland exocrine compounds of Rhodnius prolixus (Hemiptera: Reduviidae) (2002) J. Med. Entomol., 39, pp. 256-265
  • Schofield, C., Upton, C.P., Brindley's scent-glands and the metasternal scent-glands of Panstrongylus megistus (Hemiptera, Reduviidae, Triatominae) (1978) Rev. Bras. Biol., 38, pp. 665-678
  • Schofield, C.J., A comparison of sampling techniques for domestic populations of Triatominae (1978) Trans. R. Soc. Trop. Med. Hyg., 72, pp. 449-455
  • Schofield, C.J., Demonstration of isobutyric acid in some triatomine bugs (1979) Acta Trop., 36, pp. 103-105
  • Schofield, C.J., (1994) Triatominae: Biología y Control, , Publications Eurocommunica, United Kingdom
  • Staddon, B.W., The scent glands of Heteroptera (1983) Adv. Insect Physiol., 14, pp. 131-157
  • Unelius, C.R., Bohman, B., Lorenzo, M.G., Troger, A., Franke, S., Francke, W., (4S,5S)-2,2,4-Triethyl-5-methyl-1,3-dioxolane: a new volatile released by a triatomine bug (2010) Org. Lett., 12, pp. 5601-5603
  • Vitta, A.C., Bohman, B., Unelius, C.R., Lorenzo, M.G., Behavioral and electrophysiological responses of Triatoma brasiliensis males to volatiles produced in the metasternal glands of females (2009) J. Chem. Ecol., 35, pp. 1212-1221
  • Ward, J.P., A comparison of the behavioral responses of the haematophagous bug, Triatoma infestans to synthetic homologues of two naturally occurring chemicals (n- and isobutyric acid) (1981) Physiol. Entomol., 6, pp. 325-329
  • Zacharias, C., Pontes, G., Lorenzo, M., Manrique, G., Flight initiation by male Rhodnius prolixus is promoted by female odors (2010) J. Chem. Ecol., 36, pp. 449-451
  • Zeledon, R., Rabinovich, J.E., Chagas disease: An ecological appraisal with special emphasis on its insect vectors (1981) Annu. Rev. Entomol., 26, pp. 101-133


---------- APA ----------
Minoli, S., Palottini, F., Crespo, J.G. & Manrique, G. (2013) . Dislodgement effect of natural semiochemicals released by disturbed triatomines: A possible alternative monitoring tool. Journal of Vector Ecology, 38(2), 353-360.
---------- CHICAGO ----------
Minoli, S., Palottini, F., Crespo, J.G., Manrique, G. "Dislodgement effect of natural semiochemicals released by disturbed triatomines: A possible alternative monitoring tool" . Journal of Vector Ecology 38, no. 2 (2013) : 353-360.
---------- MLA ----------
Minoli, S., Palottini, F., Crespo, J.G., Manrique, G. "Dislodgement effect of natural semiochemicals released by disturbed triatomines: A possible alternative monitoring tool" . Journal of Vector Ecology, vol. 38, no. 2, 2013, pp. 353-360.
---------- VANCOUVER ----------
Minoli, S., Palottini, F., Crespo, J.G., Manrique, G. Dislodgement effect of natural semiochemicals released by disturbed triatomines: A possible alternative monitoring tool. J. Vector Ecol. 2013;38(2):353-360.