Abstract:
This paper presents the effects of 125I labelling on human chorionic gonadotropin binding ability. The results obtained showed a marked difference in the half-life of the specific radioactivity of the radiolabelled hormone comparing the one obtained by "self-displacement" analysis and that calculated considering the half-life of the radioactive isotope (60 days for 125I). The decrease in the hormone half-life (t1/2= 3.10 days) was demonstrated by binding experiments performed at various days after hormone labelling. The affinity constant (Ka) and the number of binding sites obtained after Scatchard analysis using rat testes as receptor source, remained constant when the "self-displacement" specific radioactivity was used while they varied significantly if the 60 days half-life was considered for the data processing. The importance of the labelled hormone degradation as a possible cause of the decrease in the binding ability is also described. © 1985 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
Registro:
Documento: |
Artículo
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Título: | Effects of radioiodination on hormone binding ability |
Autor: | Radicella, J.P.; Calvo, J.C.; Charreau, E.H. |
Filiación: | Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Republica, Argentina Instituto de Biologia y Medicina Experimental, Obligado 2490, Buenos Aires, 1428, Argentina
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Palabras clave: | radioisotope; chorionic gonadotropin i 125; endocrine system; hormone binding; human; human cell; methodology; priority journal; radioiodination; Animal; Binding Sites; Chorionic Gonadotropin; Chromatography, Gel; Half-Life; In Vitro; Iodine Radioisotopes; Kinetics; Male; Molecular Weight; Rats; Testis |
Año: | 1985
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Volumen: | 5
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Número: | 5-6
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Página de inicio: | 349
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Página de fin: | 365
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DOI: |
http://dx.doi.org/10.3109/10799898509041887 |
Título revista: | Journal of Receptors and Signal Transduction
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Título revista abreviado: | J. Recept. Signal Transduction
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ISSN: | 10799893
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CODEN: | JRETE
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CAS: | Chorionic Gonadotropin; Iodine Radioisotopes
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10799893_v5_n5-6_p349_Radicella |
Referencias:
- Calvo, J.C., Radicella, J.P., Charreau, E.H., Measurement of specific radioactivities in labelled hormones by self-displacement analysis (1983) Biochem. J., 212, pp. 259-264
- Merz, W., Dörner, M., Iodinated choriogonadotropin (hCG): Biological, physical and immunological investigations (1983) Acta Endocrinologica, 102, pp. 51-52
- Benveniste, R., Frohman, L.A., Rabinowitz, D., Heterogenity of purified human pituitary luteinizing hormone: effect on hormone measurement by radioimmunoassay (1975) Endocrinology, 97, pp. 20-29
- Jacobs, H.S., Lawton, N.F., Pituitary and placental glycopeptid hormones (1974) Brit. med. Bull, 30, pp. 55-61
- Rabinowitz, D., Benveniste, R., Bell, J., Heterogenity of human luteinizing hormone. Behaviour in radioimmunoassay and radioligand-receptor assay systems and evidence for the presence of a-subunit in serum (1974) Israel J. med. Sci., 10, pp. 1272-1287
- Ryan, R.J., Jiang, N.S., Hanlon, S., Some physical and hydrodynamic properties of human FSH and LH (1970) Recent Progr. Hormone Res., 26, pp. 105-137
- Van Orden, D.E., The analysis and fractionation of LH125I by gel filtration (1972) Proc. Soc. exp. Biol. (N.Y.), 139, pp. 267-270
- Evans, E.A., (1976) Self-decomposition of radiochemicals, , Amersham International, Amersham England
- Bayly, R.J., Weigel, H., Self-decomposition of compounds labelled with radioactive isotopes (1960) Nature, 188, pp. 384-387
- Redshaw, A., Lynch, B., An improved method for the preparation of iodinated antigens for radioimmunoassay (1974) J. Endocrinol., 60, pp. 527-528
- Scatchard, G., The attractions of proteins for small molecules and ions (1949) Ann. N.Y. Acad. Sci., 51, pp. 660-672
- Schlaff, S., An acrylamide gel recombination assay for glycoprotein hormone subunits (1976) Endocrinology, 98, pp. 527-533
- Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685
- Sammons, D.W., Adams, L.D., Nishizawa, E.E., Ultrasensitive silver-based colour staining of polypeptides in polyacrylamide gels (1981) Electrophoresis, 2, pp. 135-141
- Cresto, J.C., Udrisar, D.P., Camberos, M.C., Basabe, J.C., Gómez Acuń, P., De Majo, S.F., Preparation of biologically active mono125I-insulin of high specific activity (1981) Acta Physiol. Lat. Am., 31, pp. 17-24
- Parker, K.C., Strominger, J.L., Localization of the sites of iodination of human β2-microglobulin: quaternary structure implications for hystocompatibility antigens (1983) Biochem., 22, pp. 1145-1153
- Thorell, J.J., Johansson, B.G., Enzymatic iodination of polypeptides with 125I to high specific activity (1971) Biochim. Biophys. Acta, 251, pp. 363-369
Citas:
---------- APA ----------
Radicella, J.P., Calvo, J.C. & Charreau, E.H.
(1985)
. Effects of radioiodination on hormone binding ability. Journal of Receptors and Signal Transduction, 5(5-6), 349-365.
http://dx.doi.org/10.3109/10799898509041887---------- CHICAGO ----------
Radicella, J.P., Calvo, J.C., Charreau, E.H.
"Effects of radioiodination on hormone binding ability"
. Journal of Receptors and Signal Transduction 5, no. 5-6
(1985) : 349-365.
http://dx.doi.org/10.3109/10799898509041887---------- MLA ----------
Radicella, J.P., Calvo, J.C., Charreau, E.H.
"Effects of radioiodination on hormone binding ability"
. Journal of Receptors and Signal Transduction, vol. 5, no. 5-6, 1985, pp. 349-365.
http://dx.doi.org/10.3109/10799898509041887---------- VANCOUVER ----------
Radicella, J.P., Calvo, J.C., Charreau, E.H. Effects of radioiodination on hormone binding ability. J. Recept. Signal Transduction. 1985;5(5-6):349-365.
http://dx.doi.org/10.3109/10799898509041887