Abstract:
The effect of hyperprolactinemia induced by median eminence lesions (MEL) and ACTH and glucocorticoid replacement on prolactin (Prl) receptors was studied in the adrenal, isolated Langerhans islets and the liver. Adult rats were ovariectomized 15 days before MEL and they were divided in the following groups: 1) SHAM: injected with saline solution 3 times in alternate days; 2) MEL: saline solution; 3) MEL + ACTH: 50 ug: 10 IU/rat, s.c. (Synacthen) and 4) MEL + DEXA: 10 ug/rat (dexamethasone). For measuring total lactogenic binding sites an in vitro treatment of the membrane fraction with 4M MgCl2 was used. MEL originated a significant increase in Prl serum levels, which was not altered by injections of ACTH or dexamethasone. In contrast, serum corticosterone (B) levels in MEL rats were significantly lowered, and it was restored by ACTH. Unexpectedly, B levels increased when dexamethasone was administrered to MEL rats. Prl receptors were diminished in the adrenal gland and Langerhans islets from MEL animals, as compared with the SHAM group. ACTH and glucocorticoid administration did not affect the pancreatic Prl receptors, while the adrenal gland exhibited a further lowering of Prl binding sites during ACTH treatment. Since no effect was found when dexamethasone was injected, a possible direct action of ACTH is suggested. On the other hand, Prl receptors were induced in the liver by MEL, and this action was abolished by dexamethasone and ACTH. Binding affinity in every tissue studied remained unchanged. Our data suggest that endogenous Prl is able to regulate its own receptors not only in the liver, but also in the adrenal gland and pancreatic islets. © 1985 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
Registro:
Documento: |
Artículo
|
Título: | Effect of median eminence lesions and hormonal replacement on the prolactin receptors in the adrenal Gland and langerhans islets from ovariectomized adult rats |
Autor: | Lüthy, I.A.; Tesone, M.; Oliveira-Filho, R.M.; Somoza, G.M.; Charreau, E.H.; Libertun, C.; Calandra, R.S. |
Filiación: | Instituto de Biología y Medicina Experimental, Obligado 2490, 1428, Buenos Aires, Argentina
|
Palabras clave: | corticotropin; dexamethasone; prolactin i 125; prolactin receptor; radioisotope; adrenal gland; animal cell; central nervous system; dose response; drug receptor binding; drug response; endocrine system; female genital system; hyperprolactinemia; median eminence; nonhuman; ovariectomy; pancreas islet; pharmacokinetics; priority journal; rat; Adrenal Glands; Adrenocorticotropic Hormone; Animals; Castration; Dexamethasone; Female; Islets of Langerhans; Liver; Median Eminence; Prolactin; Rats; Receptors, Cell Surface; Receptors, Prolactin |
Año: | 1985
|
Volumen: | 5
|
Número: | 1
|
Página de inicio: | 105
|
Página de fin: | 119
|
DOI: |
http://dx.doi.org/10.3109/10799898509041873 |
Título revista: | Journal of Receptors and Signal Transduction
|
Título revista abreviado: | J. Recept. Signal Transduction
|
ISSN: | 10799893
|
CODEN: | JRETE
|
CAS: | corticotropin, 11136-52-0, 9002-60-2, 9061-27-2; dexamethasone, 50-02-2; Adrenocorticotropic Hormone, 9002-60-2; Dexamethasone, 50-02-2; Prolactin, 9002-62-4; Receptors, Cell Surface; Receptors, Prolactin
|
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10799893_v5_n1_p105_Luthy |
Referencias:
- Marshall, S., Gelato, M., Meites, J., Serum prolactin and prolactin binding activity in adrenals and kidneys of male rats after dehydration, salt loading and unilateral nephrectomy (1975) Proc. Soc. Exp. Biol. Med., 149, pp. 185-188
- Calvo, J.C., Finocchiaro, L., Lüthy, I., Charreau, E.H., Calandra, R., Engström, B., Hansson, V., Specific prolactin binding in the rat adrenal gland: its characterization and hormonal regulation (1981) J. Endocrinol., 89, pp. 317-325
- Tesone, M., Oliveira-Filho, R.M., Charreau, E.H., Prolactin binding in rat Langerhans islets (1980) J. Recept. Res., 1, pp. 355-372
- Shiu, R.P.C., Friesen, H.G., Properties of a prolactin receptor from the rabbit mammary gland (1974) J., 140, pp. 301-311
- Posner, B.I., Kelly, P.A., Shiu, R.P., Friesen, H.G., Studies of insulin, growth hormone and prolactin binding: tissue distributions species variation and characterization (1974) Endocrinology, 95, pp. 521-531
- Witorsch, R.J., Kitay, J.I., Pituitary hormones affecting adrenal 5 -reductase activity: ACTH, growth hormone and prolactin (1972) Endocrinology, 91, pp. 764-769
- Gustafsson, J.A., Stenberg, A., Influence of prolactin on the metabolism of steroid hormones in rat liver and adrenals (1975) Acta Endocrinol. (Copenh), 78, pp. 545-553
- Mann, D.R., Cost, M.G., Jacobson, C.D., Macfarland, L.A., Adrenal gland rhythmicity and pituitary regulation of adrenal steroid secretion (1977) Proc. Soc. Exper. Biol. Med., 156, pp. 441-445
- Advis, J., Ojeda, S.R., Hyperprolactinemia-induced precocious puberty in the female rat: ovarian site of action (1978) Endocrinology, 103, pp. 924-935
- Piva, F., Galgiano, P., Motta, M., Martini, L., Adrenal progesterone: factors controlling its secretion (1973) Endocrinology, 93, pp. 1178-1184
- Rathgeb, I.B., Winkler, B., Steele, R., Altszuler, N., Effect of ovine prolactin administration on glucose metabolism and plasma insulin levels in the dog (1971) Endocrinology, 88, pp. 718-722
- Landgraf, R., Landgraf-Leurs, M.M.C., Wessman, A., Hörl, R., Von Werder, K., Scriba, P.C., Prolactin: a diabetogenic hormone (1977) Diabetologia, 13, pp. 99-104
- Costrini, N.V., Kalkhoff, R.K., Relative effects of pregnancy, estradiol and progesterone on plasma insulin and pancreatic islet insulin secretion (1971) J. Clin. Invest., 50, pp. 992-999
- Malaise, W.J., Hormonal and environmental modification of islet activity (1972) Handbook of Physiology, I, pp. 237-260. , edited by R.O. Greep, E.B. Astwood. American Physiological Society, Washington, Sec 7
- Nielsen, J.H., Effects of growth hormone, prolactin and placental lactogen on insulin content and release, and deoxyribonucleic acid synthesis in culture pancreatic islets (1982) Endocrinology, 110, pp. 600-605
- Tesone, M., Lüthy, I.A., Ladenheim, R.G., Calandra, R.S., Charreau, E.H., Regulation of prolactin binding sites in the endocrine pancreas and adrenal glands of ovariectomized adult rats: effect of prolactin, sulpiride and bromocriptine administration J. Recept. Res, , in press
- Thorell, J.I., Johansson, B.G., Enzymatic iodination of polypeptides with 125I to high specific activity (1971) Biochem. Biophys. Acta, 251, pp. 363-369
- Charreau, E.H., Attramadal, A., Torjesen, P.A., Calandra, R., Purvis, K., Hansson, V., Androgen stimulation of prolactin receptors in rat prostate (1977) Mol. Cell. Endocrinol., 7, pp. 1-7
- Libertun, C., Kaplan, S.E., De Nicola, A.F., Progesterone negative feedback on prolactin secretion: importance of the brain control and of estradiol (1979) Neuroendocrinology, 28, pp. 64-70
- Lacy, P.E., Kostianovsky, M., Method for the isolation of intact islets of Langerhans from the rat pancreas (1967) Diabetes, 16, pp. 35-39
- Tesone, M., Chazenbalk, G., Ballejos, G., Charreau, E.H., Estrogen receptor in rat pancreatic islets (1979) J. Ster. Biochem., 11, pp. 1309-1314
- Lüthy, I.A., Chiauzzi, V.A., Charreau, E.H., Calandra, R.S., Effects of prolactin, bromocriptine and sulpiride on estrogen and lactogenic receptors in the rat adrenal gland Acta Physiol. Latinoamer., , in press
- Murphy, B.P., Engelberg, W., Pattee, C.J., Simple method for the determination of plasma corticoids (1963) J. Clin. Endocrinol. Metab., 23, pp. 293-300
- Murphy, B.E.P., Some studies of the protein-binding of steroids and their application to the routine micro and ultramicro measurement of various steroids in body fluids by competitive protein-binding radioassay (1967) J. Clin. Endocrinol. Metab., 27, pp. 973-980
- Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the Folin phenol reagent (1951) J. Biol. Chem., 193, pp. 265-275
- Keightley, D.D., Cressie, N.A.C., The Woolf plot is more reliable than the Scatchard plot in analyzing data from hormone receptor assays (1980) J. Steroid Biochem., 13, pp. 1317-1323
- Li, C.C., (1964) Introduction to experimental statistics, , Mc Graw Hill, New York
- Lison, L., Análisis de la varianza para varios grupos y subgrupos Planes experimentales para la comparación global y para las comparaciones individuales de los grupos experimentales. In: Estadística aplicada a la biología experimental (1976) Planificación de experiencias y análisis de los resultados, pp. 89-132. , EUDEBA, Buenos Aires
- Fiszer de Plazas, S., Becú, D., Mitridate de Novara, A., Libertun, C., Aminobutyric acid receptors in anterior pituitary and brain areas after median eminence lesions (1982) Endocrinology, 111, pp. 1974-1978
- Morris, P., Saxena, B., Dose-response effects of prolactin (PRL) on LH and PRL binding sites in rat Leydig homogenates (1979) The Endocrine Society, 61st Ann. Meeting, , Abst 164
- Davies, R.F., Katikineni, M., Chan, V., Harwood, J.P., Dufau, M.L., Catt, K.J., Lactogenic receptor regulation in hormone-stimulated steroidogenic cells (1980) Nature, 283, pp. 863-865
- Loose, D.S., Do, Y.S., Chen, T.L., Feldman, D., Demonstration of glucocorticoid receptors in the adrenal cortex: evidence for a direct dexamethasone suppressive effect on the rat adrenal gland (1980) Endocrinology, 107, pp. 137-146
- Marshall, S., Huang, H.H., Kledzik, G.S., Campbell, G.A., Meites, J., Glucocorticoid regulation of prolactin receptors in kidneys and adrenals of male rats (1978) Endocrinology, 102, pp. 869-875
- Costlow, M.E., McGuire, W.L., Prolactin stimulation of prolactin receptors in rat liver (1975) Life Sci, 17, pp. 1457-1466
- Manni, A., Chambers, M.J., Pearson, O.H., Prolactin induces its own receptors in rat liver (1978) Endocrinology, 103, pp. 2168-2171
- Posner, B.I., Kelly, P.A., Friesen, H.G., Prolactin receptors in rat liver: possible induction by prolactin (1975) Science, 188, pp. 57-59
- Djiane, J., Durand, P., Prolactin-progesterone antagonism in self regulation of prolactin receptors in the mammary gland (1977) Nature, 266, pp. 641-643
- Djiane, J., Delouis, C., Kelly, P.A., Prolactin receptors in organ culture of rabbit mammary gland: effect of cycloheximide and prolactin (1979) Proc. Soc. Exper. Biol. Med., 162, pp. 342-345
- Ogle, R.F., Kitay, J.I., Interactions of prolactin and adrenocortical secretions in female rats (1979) Endocrinology, 104, pp. 40-44
Citas:
---------- APA ----------
Lüthy, I.A., Tesone, M., Oliveira-Filho, R.M., Somoza, G.M., Charreau, E.H., Libertun, C. & Calandra, R.S.
(1985)
. Effect of median eminence lesions and hormonal replacement on the prolactin receptors in the adrenal Gland and langerhans islets from ovariectomized adult rats. Journal of Receptors and Signal Transduction, 5(1), 105-119.
http://dx.doi.org/10.3109/10799898509041873---------- CHICAGO ----------
Lüthy, I.A., Tesone, M., Oliveira-Filho, R.M., Somoza, G.M., Charreau, E.H., Libertun, C., et al.
"Effect of median eminence lesions and hormonal replacement on the prolactin receptors in the adrenal Gland and langerhans islets from ovariectomized adult rats"
. Journal of Receptors and Signal Transduction 5, no. 1
(1985) : 105-119.
http://dx.doi.org/10.3109/10799898509041873---------- MLA ----------
Lüthy, I.A., Tesone, M., Oliveira-Filho, R.M., Somoza, G.M., Charreau, E.H., Libertun, C., et al.
"Effect of median eminence lesions and hormonal replacement on the prolactin receptors in the adrenal Gland and langerhans islets from ovariectomized adult rats"
. Journal of Receptors and Signal Transduction, vol. 5, no. 1, 1985, pp. 105-119.
http://dx.doi.org/10.3109/10799898509041873---------- VANCOUVER ----------
Lüthy, I.A., Tesone, M., Oliveira-Filho, R.M., Somoza, G.M., Charreau, E.H., Libertun, C., et al. Effect of median eminence lesions and hormonal replacement on the prolactin receptors in the adrenal Gland and langerhans islets from ovariectomized adult rats. J. Recept. Signal Transduction. 1985;5(1):105-119.
http://dx.doi.org/10.3109/10799898509041873