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Abstract:

The role of prolactin (Prl) in the regulation of Prl binding to its specific binding sites was studied in the Langerhans islets, adrenal gland and liver of adult ovariec tomized female rats. Animals were sc injected twice daily during 10 days with ovine Prl (1 mg/kg BW), sulpiride (30 mg/kg BW) and bromocriptine (3 mg/kg BW). At the end of the treatment period, the animals were killed and serum was collected for Prl assay. Total Prl binding sites were measured in the membrane fraction of tissue by desaturating the occupied membrane receptors in vitro with 4M MgCl2. Serum levels of Prl were significantly higher in sulpiride-treated animals, whereas bromocriptine administration rendered undetectable values. Prolactin and sulpiride treatment significantly reduced Prl binding to the adrenal gland and Langerhans islets, whereas it greatly increased Prl binding to the liver. On the other hand, bromocriptine increased Prl binding sites in the adrenal gland and Langerhans islets, but in the liver caused no apparent effect. The binding affinity (Ka) in each tissue remained unchanged under the different experimental conditions. In addition, the binding of Prl to pancreas islets membranes was lower in late pregnancy when compared with control rats. All of these data provide strong evidence in favor of a role for Prl in regulating the number of its own tissue binding sites. © 1983 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

Registro:

Documento: Artículo
Título:Prolactin regulation of prolactin binding sites in pancreatic islets and adrenal glands of ovariectomized rats
Autor:Tesone, M.; Luthy, I.A.; Ladenheim, R.G.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Instituto de Biología, Medicina Experimental, Obligado 2490, 1428, Buenos Aires, Argentina
Palabras clave:bromocriptine; hormone receptor; magnesium chloride; prolactin; prolactin i 125; prolactin receptor; radioisotope; sulpiride; adrenal gland; animal cell; drug receptor binding; endocrine system; female genital system; nonhuman; ovariectomy; pancreas islet; pharmacokinetics; rat; Adrenal Glands; Animal; Bromocriptine; Comparative Study; Female; Islets of Langerhans; Liver; Ovary; Prolactin; Rats; Rats, Inbred Strains; Receptors, Cell Surface; Receptors, Prolactin; Sulpiride; Support, Non-U.S. Gov't
Año:1983
Volumen:3
Número:6
Página de inicio:711
Página de fin:726
DOI: http://dx.doi.org/10.3109/10799898309041956
Título revista:Journal of Receptors and Signal Transduction
Título revista abreviado:J. Recept. Signal Transduction
ISSN:10799893
CODEN:JRETE
CAS:bromocriptine, 25614-03-3; magnesium chloride, 7786-30-3, 7791-18-6; prolactin, 12585-34-1, 50647-00-2, 9002-62-4; sulpiride, 15676-16-1; Bromocriptine, 25614-03-3; Prolactin, 9002-62-4; Receptors, Cell Surface; Receptors, Prolactin; Sulpiride, 15676-16-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10799893_v3_n6_p711_Tesone

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Citas:

---------- APA ----------
Tesone, M., Luthy, I.A. & Ladenheim, R.G. (1983) . Prolactin regulation of prolactin binding sites in pancreatic islets and adrenal glands of ovariectomized rats. Journal of Receptors and Signal Transduction, 3(6), 711-726.
http://dx.doi.org/10.3109/10799898309041956
---------- CHICAGO ----------
Tesone, M., Luthy, I.A., Ladenheim, R.G. "Prolactin regulation of prolactin binding sites in pancreatic islets and adrenal glands of ovariectomized rats" . Journal of Receptors and Signal Transduction 3, no. 6 (1983) : 711-726.
http://dx.doi.org/10.3109/10799898309041956
---------- MLA ----------
Tesone, M., Luthy, I.A., Ladenheim, R.G. "Prolactin regulation of prolactin binding sites in pancreatic islets and adrenal glands of ovariectomized rats" . Journal of Receptors and Signal Transduction, vol. 3, no. 6, 1983, pp. 711-726.
http://dx.doi.org/10.3109/10799898309041956
---------- VANCOUVER ----------
Tesone, M., Luthy, I.A., Ladenheim, R.G. Prolactin regulation of prolactin binding sites in pancreatic islets and adrenal glands of ovariectomized rats. J. Recept. Signal Transduction. 1983;3(6):711-726.
http://dx.doi.org/10.3109/10799898309041956