Artículo

Tesone, M.; Oliveira-Filho, R.M.; Charreau, E.H. "Proiactin binding in rat langerhans islets" (1980) Journal of Receptors and Signal Transduction. 1(3):355-372
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Abstract:

Membrane preparations of collagenase-dispersed Langerhans islets of female Wistar rats exhibit specific binding sites for 125I labelled ovine prolactin (125I-oPrl). Almost negligible binding was detected in islets of male animals. The binding is a saturable and time-temperature dependent process, equilibrium being reached after 16 h incubation at 0°C. The bound oPrl is not displaceable by hFSH, hLH, bGH or hGH. In contrast with other cell fractions, the 12,000 g pellet accounts for more than 80% of the specific binding of 125I-oPrl. Scatchard plots of data obtained in saturation studies indicate a single class of binding sites with Ka = 0.21 × 1010 M-1. Protein and phospholipid moieties are essential for the receptor activity, since after trypsin or phospholipase C digestions marked loss of binding was verified. In islets of streptozotocin diabetic rats a marked reduction in the number of binding sites was observed. These findings may suggest that some of the actions of prolactin on endocrine pancreas could be explained by its specific interaction with islet cell membranes. © 1980 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

Registro:

Documento: Artículo
Título:Proiactin binding in rat langerhans islets
Autor:Tesone, M.; Oliveira-Filho, R.M.; Charreau, E.H.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas, Naturales Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:prolactin; prolactin i 125; radioisotope; animal experiment; cell membrane; endocrine system; in vitro study; pancreas islet cell; rat; Animal; Cell Membrane; Diabetes Mellitus, Experimental; Female; Islets of Langerhans; Male; Phospholipase C; Prolactin; Protease Inhibitors; Rats; Rats, Inbred Strains; Receptors, Cell Surface; Receptors, Prolactin; Sex Factors; Temperature; Trypsin
Año:1980
Volumen:1
Número:3
Página de inicio:355
Página de fin:372
DOI: http://dx.doi.org/10.3109/10799898009038787
Título revista:Journal of Receptors and Signal Transduction
Título revista abreviado:J. Recept. Signal Transduction
ISSN:10799893
CODEN:JRETE
CAS:prolactin, 12585-34-1, 50647-00-2, 9002-62-4; Phospholipase C, EC 3.1.4.3; Prolactin, 9002-62-4; Protease Inhibitors; Receptors, Cell Surface; Receptors, Prolactin; Trypsin, EC 3.4.21.4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10799893_v1_n3_p355_Tesone

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Citas:

---------- APA ----------
Tesone, M., Oliveira-Filho, R.M. & Charreau, E.H. (1980) . Proiactin binding in rat langerhans islets. Journal of Receptors and Signal Transduction, 1(3), 355-372.
http://dx.doi.org/10.3109/10799898009038787
---------- CHICAGO ----------
Tesone, M., Oliveira-Filho, R.M., Charreau, E.H. "Proiactin binding in rat langerhans islets" . Journal of Receptors and Signal Transduction 1, no. 3 (1980) : 355-372.
http://dx.doi.org/10.3109/10799898009038787
---------- MLA ----------
Tesone, M., Oliveira-Filho, R.M., Charreau, E.H. "Proiactin binding in rat langerhans islets" . Journal of Receptors and Signal Transduction, vol. 1, no. 3, 1980, pp. 355-372.
http://dx.doi.org/10.3109/10799898009038787
---------- VANCOUVER ----------
Tesone, M., Oliveira-Filho, R.M., Charreau, E.H. Proiactin binding in rat langerhans islets. J. Recept. Signal Transduction. 1980;1(3):355-372.
http://dx.doi.org/10.3109/10799898009038787