Abstract:
Membrane preparations of collagenase-dispersed Langerhans islets of female Wistar rats exhibit specific binding sites for 125I labelled ovine prolactin (125I-oPrl). Almost negligible binding was detected in islets of male animals. The binding is a saturable and time-temperature dependent process, equilibrium being reached after 16 h incubation at 0°C. The bound oPrl is not displaceable by hFSH, hLH, bGH or hGH. In contrast with other cell fractions, the 12,000 g pellet accounts for more than 80% of the specific binding of 125I-oPrl. Scatchard plots of data obtained in saturation studies indicate a single class of binding sites with Ka = 0.21 × 1010 M-1. Protein and phospholipid moieties are essential for the receptor activity, since after trypsin or phospholipase C digestions marked loss of binding was verified. In islets of streptozotocin diabetic rats a marked reduction in the number of binding sites was observed. These findings may suggest that some of the actions of prolactin on endocrine pancreas could be explained by its specific interaction with islet cell membranes. © 1980 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
Registro:
Documento: |
Artículo
|
Título: | Proiactin binding in rat langerhans islets |
Autor: | Tesone, M.; Oliveira-Filho, R.M.; Charreau, E.H. |
Filiación: | Departamento de Química Biológica, Facultad de Ciencias Exactas, Naturales Universidad de Buenos Aires, Buenos Aires, Argentina
|
Palabras clave: | prolactin; prolactin i 125; radioisotope; animal experiment; cell membrane; endocrine system; in vitro study; pancreas islet cell; rat; Animal; Cell Membrane; Diabetes Mellitus, Experimental; Female; Islets of Langerhans; Male; Phospholipase C; Prolactin; Protease Inhibitors; Rats; Rats, Inbred Strains; Receptors, Cell Surface; Receptors, Prolactin; Sex Factors; Temperature; Trypsin |
Año: | 1980
|
Volumen: | 1
|
Número: | 3
|
Página de inicio: | 355
|
Página de fin: | 372
|
DOI: |
http://dx.doi.org/10.3109/10799898009038787 |
Título revista: | Journal of Receptors and Signal Transduction
|
Título revista abreviado: | J. Recept. Signal Transduction
|
ISSN: | 10799893
|
CODEN: | JRETE
|
CAS: | prolactin, 12585-34-1, 50647-00-2, 9002-62-4; Phospholipase C, EC 3.1.4.3; Prolactin, 9002-62-4; Protease Inhibitors; Receptors, Cell Surface; Receptors, Prolactin; Trypsin, EC 3.4.21.4
|
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10799893_v1_n3_p355_Tesone |
Referencias:
- Nielsen, J.H., Brunstedt, K., Jørgensen, R., Jensen, D., - Direct long-term effect of growth hormone and prolactin on growth and function of pancreatic islets in tissue culture (1978) Diabetologia, 15, p. 258. , (Abstract)
- Kahn, C.R., Goldfine, I.D., Neville, D.M., Jr., Demeyts, P., - Alterations in insulin binding induced by changes in vivo in the levels of glucocorticoids and growth hormone (1978) Endocrinology, 103, pp. 1054-1066
- Manns, J.D., Boda, J.M., - Effects of ovine growth hormone and prolactin on blood glucose, serum insulin, plasma non-sterified fatty acids and amino nitrogen in sheep (1965) Endocrinology, 76, pp. 1109-1114
- Rathgeb, I.B., Winkler, B., Steele, R., Altszuler, N., - Effect of ovine prolactin administration on glucose metabolism and plasma insul in levels in the dog (1971) Endocrinology, 88, pp. 718-722
- Funk, C., Chamelin, I.M., Wagreich, H., Harrow, B., - A study of hormonal factors which influence the production of insulin (1941) Science, 94, pp. 260-261
- Houssay, B.A., Anderson, E., Bates, R.W., Li, C.H., - Diabetogenic action of prolactin (1955) Endocrinology, 57, pp. 55-63
- Landgraf, R., Landgraf-Leurs, M.M.C., Weissmann, A., Hörl, R., von Werder, K., Scriba, P.C., - Prolactin: a diabetogenic hormone (1977) Diabetologia, 13, pp. 99-104
- Sonenberg, M., Money, W.L., Keston, A.S., Fitzgerald, P.J., Godwin, J.T., - Localization of radioactivity after administration of labeled prolactin preparations to the female rat (1951) Endocrinology, 49, pp. 709-719
- Sonenberg, M., Money, W.L., Dorans, J.F., Lucas, V., Bourgne, L., - The distribution of radioactivity in the tissues of the rat after the administration of radioactive growth hormone preparations (1954) Endocrinology, 55, pp. 709-720
- Sonenberg, M., Money, W.L., - The fate and metabolism of anterior pituitary hormones (1955) Rec. Prog. Horm. Res, 11, pp. 43-82
- Shiu, R.P.C., Friesen, H.G., - Properties of a prolactin receptor from the rabbit mammary gland (1974) Biochem. J, 140, pp. 301-311
- Oliveira-Filho, R.M., Tesone, M., Charreau, E.H., - Receptores de prolactina en islotes de Langerhans (1979) Proc. of the V Argentine Congress on Endocrinology and Metabolism, p. 30. , (Abstract)
- Tesone, M., Oliveira-Filho, R.M., Chazenbalk, G.D., Ballejos, G., Charreau, E.H., - Receptors for prolactin and estrogens in rat Langerhans islets (1979) Proc. of the 31st Annual Meeting of the Brazilian Soc. for the Advancement of Science (SBPC), , (Abstract #32-G.1.5)
- Dellacha, J.M., Sonenberg, M., - Purification of bovine growth hormone (1964) J. Biol. Chem, 239, pp. 1515-1520
- Turyn, D., Santomé, J.A., Dellacha, M., - Specific binding of human and bovine growth hormones to hypophysectomized rat hepatocytes (1976) Acta Physiol. Latinoamer, 26, pp. 395-402
- Tesone, M., Valle, L.B.S., Foglia, V.G., Charreau, E.H., - Endocrine function of the testis in streptozotocin diabetic rats (1976) Acta Physiol. Latinoamer, 26, pp. 387-394
- Charreau, E.H., Calvo, J.C., Tesone, M., Valle, L.B.S., Barañao, J.L., - Insulin regulation of Leydig cell luteinizing hormone receptors (1978) J. Biol. Chem, 253, pp. 2504-2506
- Calvo, J.C., Valle, L.B.S., Barañao, J.L., Tesone, M., Charreau, E.H., - NADPH-Generating enzymes in Leydig cells from diabetic rats (1979) Horm. Metab. Res, 11, pp. 161-164
- Lacy, P.E., Kostianovsky, M., - Method for the isolation of intact islets of Langerhans from the rat pancreas (1967) Diabetes, 16, pp. 35-39
- Gagliardino, J.J., Pessacq, M.T., Hernández, R.E., - Insulin and cyclic AMP release by isolated-incubated islets of Langerhans (1977) J. Endocr, 73, pp. 179-180
- Tesone, M., Chazenbalk, G., Ballejos, G., Charreau, E.H., - Estrogen receptor in rat pancreatic islets (1979) J. Ster. Biochem, 11, pp. 1309-1314
- Sernia, C., Tyndale-Biscoe, C.H., - Prolactin receptors in the mammary gland, corpus luteum and other tissues of the tammar wallaby, Macropus eugenii. J (1979) Endocr, 83, pp. 79-89
- Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., - Protein measurement with the Folin phenol reagent (1951) J. Biol. Chem, 193, pp. 265-275
- Thorell, J.I., Johansson, B.G., - Enzymatic iodination of polypeptides with 125I to high specific activity (1971) Biochim. Biophys. Acta, 251, pp. 363-369
- Charreau, E.H., Attramadal, A., Torjesen, P.A., Calandra, R., Purvis, K., Hansson, V., - Androgen stimulation of prolactin receptors in rat prostate (1977) Mol. Cell. Endocrinol, 7, pp. 1-7
- Scatchard, G., - The attraction of proteins for small molecules and ions (1949) Ann. N.Y. Acad. Sci, 51, pp. 660-672
- Kelly, P.A., Posner, B.I., Tsushima, T., Friesen, H.G., - Studies of insulin, growth hormone and prolactin binding: ontogenesis, effects of sex and pregnancy (1974) Endocrinology, 95, pp. 532-539
- Aragona, C., Friesen, H.G., - Specific prolactin binding sites in the prostate and testis of rats (1975) Endocrinology, 97, pp. 677-684
- Kledzik, G.S., Marshall, S., Campbell, G.A., Gelato, M., Meites, J., - Effects of castration, testosterone, estradiol, and prolactin on specific prolactin-binding activity in ventral prostate of male rats (1976) Endocrinology, 98, pp. 373-379
- Barkey, R.J., Shani, J., Amit, T., Barzilai, D., - Specific binding of prolactin to seminal vesicle, prostate and testicular homogenates of immature, mature and aged rats (1977) J. Endocr, 74, pp. 163-173
- Posner, B.I., Kelly, P.A., Friesen, H.G., - Induction of a lactogenic receptor in rat liver: influence of estrogen and the pituitary (1974) Proc. Nat. Acad. Sci. USA, 71, pp. 2407-2410
- Charreau, E.H., Attramadal, A., Torjesen, P.A., Purvis, K., Calandra, R., Hansson, V., - Prolactin binding in rat testis: specific receptors in interstitial cells (1977) Mol. Cell. Endocrinol, 6, pp. 303-307
- Gelato, M., Marshall, S., Boudreau, M., Bruni, J., Campbell, G.A., Meites, J., - Effects of thyroid and ovaries on prolactin binding activity in rat liver (1975) Endocrinology, 96, pp. 1292-1296
Citas:
---------- APA ----------
Tesone, M., Oliveira-Filho, R.M. & Charreau, E.H.
(1980)
. Proiactin binding in rat langerhans islets. Journal of Receptors and Signal Transduction, 1(3), 355-372.
http://dx.doi.org/10.3109/10799898009038787---------- CHICAGO ----------
Tesone, M., Oliveira-Filho, R.M., Charreau, E.H.
"Proiactin binding in rat langerhans islets"
. Journal of Receptors and Signal Transduction 1, no. 3
(1980) : 355-372.
http://dx.doi.org/10.3109/10799898009038787---------- MLA ----------
Tesone, M., Oliveira-Filho, R.M., Charreau, E.H.
"Proiactin binding in rat langerhans islets"
. Journal of Receptors and Signal Transduction, vol. 1, no. 3, 1980, pp. 355-372.
http://dx.doi.org/10.3109/10799898009038787---------- VANCOUVER ----------
Tesone, M., Oliveira-Filho, R.M., Charreau, E.H. Proiactin binding in rat langerhans islets. J. Recept. Signal Transduction. 1980;1(3):355-372.
http://dx.doi.org/10.3109/10799898009038787