Abstract:
Acquisition of invasive/metastatic potential is a key event in tumor progression. Cell surface glycoproteins and their respective matrix ligands have been implicated in this process. Recent evidence reveals that the secreted glycoprotein SPARC (secreted protein, acidic and rich in cysteine) is highly expressed in different malignant tissues. The present study reports that the suppression of SPARC expression by human melanoma cells using a SPARC antisense expression vector results in a significant decrease in the in vitro adhesive and invasive capacities of tumor cells, completely abolishing their in vivo tumorigenicity. This is the first evidence that SPARC plays a key role in human melanoma invasive-metastatic phenotype development.
Registro:
Documento: |
Artículo
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Título: | Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells |
Autor: | Fernanda Ledda, M.; Adris, S.; Bravo, A.I.; Kairiyama, C.; Bover, L.; Chernajovsky, Y.; Mordoh, J.; Podhajcer, O.L. |
Filiación: | Lnstituto de Invest. Bioquimicas, Fundacion Campomar, IIBBA-CONICET, Patricias Argentinas 435, 1405 Buenos Aires, Argentina Fac. Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina Hospital E. Peron, Balcarce 900, 1650 Provincia de Buenos Aires, Argentina Kennedy Institute of Rheumatology, 6 Bute Gardens, London W6 7DW, United Kingdom
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Palabras clave: | complementary rna; glycoprotein; osteonectin; animal experiment; article; cancer inhibition; cancer invasion; controlled study; human; human cell; melanoma cell; metastasis; mouse; nonhuman; priority journal; Animals; Cell Adhesion; Cell Division; Cell Movement; Down-Regulation; Gene Expression Regulation, Neoplastic; Humans; Melanoma; Melanoma, Experimental; Mice; Oligonucleotides, Antisense; Osteonectin; Transfection; Tumor Cells, Cultured; Animalia |
Año: | 1997
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Volumen: | 3
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Número: | 2
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Página de inicio: | 171
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Página de fin: | 176
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DOI: |
http://dx.doi.org/10.1038/nm0297-171 |
Título revista: | Nature Medicine
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Título revista abreviado: | NAT. MED.
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ISSN: | 10788956
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CODEN: | NAMEF
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CAS: | Oligonucleotides, Antisense; Osteonectin
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10788956_v3_n2_p171_FernandaLedda |
Referencias:
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Citas:
---------- APA ----------
Fernanda Ledda, M., Adris, S., Bravo, A.I., Kairiyama, C., Bover, L., Chernajovsky, Y., Mordoh, J.,..., Podhajcer, O.L.
(1997)
. Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells. Nature Medicine, 3(2), 171-176.
http://dx.doi.org/10.1038/nm0297-171---------- CHICAGO ----------
Fernanda Ledda, M., Adris, S., Bravo, A.I., Kairiyama, C., Bover, L., Chernajovsky, Y., et al.
"Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells"
. Nature Medicine 3, no. 2
(1997) : 171-176.
http://dx.doi.org/10.1038/nm0297-171---------- MLA ----------
Fernanda Ledda, M., Adris, S., Bravo, A.I., Kairiyama, C., Bover, L., Chernajovsky, Y., et al.
"Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells"
. Nature Medicine, vol. 3, no. 2, 1997, pp. 171-176.
http://dx.doi.org/10.1038/nm0297-171---------- VANCOUVER ----------
Fernanda Ledda, M., Adris, S., Bravo, A.I., Kairiyama, C., Bover, L., Chernajovsky, Y., et al. Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells. NAT. MED. 1997;3(2):171-176.
http://dx.doi.org/10.1038/nm0297-171